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Rapid identification of bacterial species is crucial in medicine and food hygiene. In order to achieve rapid and label-free identification of bacterial species at the single bacterium level, we propose and experimentally demonstrate an…

Antimicrobial susceptibility testing (AST) is a standard clinical procedure used to quantify antimicrobial resistance (AMR). Currently, the gold standard method requires incubation for 18-24 h and subsequent inspection for growth by a…

Chip-based Evanescent Light Scattering (cELS) utilizes the multiple modes of a high-index contrast optical waveguide for near-field illumination of unlabeled samples, thereby repositioning the highest spatial frequencies of the sample into…

Reflection phase imaging provides label-free, high-resolution characterization of biological samples, typically using interferometric-based techniques. Here, we investigate reflection phase microscopy from intensity-only measurements under…

Quantitative Methods · Quantitative Biology 2019-12-18 Alex Matlock , Anne Sentenac , Patrick C. Chaumet , Ji Yi , Lei Tian

A Rapid and cost-effective method for detecting bacterial cells on surfaces is critical to protect public health from various aspects, including food safety, clinical hygiene, and pharmacy quality. Herein, we first established an optical…

Quantitative Methods · Quantitative Biology 2024-03-13 Yuzhen Zhang , Zili Gao , Lili He

There is an urgent need for the development of novel and truly rapid (equal or less than 1 hour) antibiotic susceptibility testing (AST) platforms in order to provide best antimicrobial prescribing practices and to help reduce the…

Subcellular Processes · Quantitative Biology 2025-01-22 Allen Fraiman , Lawrence Ziegler

Rapid identification of bacteria is essential to prevent the spread of infectious disease, help combat antimicrobial resistance, and improve patient outcomes. Raman optical spectroscopy promises to combine bacterial detection,…

A method of the mid-IR-laser microscopy has been proposed for the investigation of the large-scale electrically and recombination active defects in semiconductors and non-destructive inspection of semiconductor materials and structures in…

Materials Science · Physics 2011-05-17 V. P. Kalinushkin , V. A. Yuryev , O. V. Astafiev

The worldwide increase of antimicrobial resistance (AMR) is a serious threat to human health. To avert the spread of AMR, fast reliable diagnostics tools that facilitate optimal antibiotic stewardship are an unmet need. In this regard,…

We present a computational live bacteria detection system that periodically captures coherent microscopy images of bacterial growth inside a 60 mm diameter agar-plate and analyzes these time-lapsed holograms using deep neural networks for…

The elastic scattering process $\Lambda p\to\Lambda p$ offers insights on multiple problems in nuclear physics. $\mathrm{SU}(3)_F$ symmetry implies a close agreement between the $\Lambda p$ and $pp$ scattering cross sections. The $\Lambda…

Nuclear Experiment · Physics 2019-09-04 John W. Price

Dynamic light scattering techniques are routinely used for numerous industrial and research applications, because they can give access to the motion spectrum of micro- and nano-objects, and therefore to particle sizes or visco-elastic…

Optics · Physics 2022-09-22 Guillaume Graciani , John T. King , Francois Amblard

Gram staining has been one of the most frequently used staining protocols in microbiology for over a century, utilized across various fields, including diagnostics, food safety, and environmental monitoring. Its manual procedures make it…

We describe a self calibrating optical technique that allows to perform absolute measurements of scattering cross sections for the light scattered at extremely small angles. Very good performances are obtained by using a very simple optical…

Optics · Physics 2009-11-10 Doriano Brogioli , Alberto Vailati , Marzio Giglio

We demonstrate the abilities of elastic x-ray scattering to yield information on dense matter with multiple ion species and on the microscopic mixing in dense materials. Based on partial structure factors from ab initio simulations, a novel…

Plasma Physics · Physics 2015-05-19 K. Wünsch , J. Vorberger , G. Gregori , D. O. Gericke

Elastic light scattering has been extensively used to study samples showing a non uniform refraction index on lengthscales from a fraction of a micrometer to a fraction of a millimeter. Typically, a wide laser beam is sent through the…

Optics · Physics 2009-07-21 Doriano Brogioli

Raman spectroscopy is a promising tool for microbial identification, yet its implementation in microbiology and clinical workflow is still restricted due to the accompanying additional preparation required to focus on microbial signals.…

Quantitative Methods · Quantitative Biology 2026-03-19 Jeong Hee Kim , Jia Dong , Marissa Morales , Loza Tadesse

While scattered light conveys most of the information we perceive, scattering may also distort that information before it reaches our detectors. The problem is acute in many applications, such as in high-resolution microscopy of biological…

The simulation of transmission electron microscopy (TEM) images or diffraction patterns is often required to interpret their contrast and extract specimen features. This is especially true for high-resolution phase-contrast imaging of…

Materials Science · Physics 2021-03-30 Jacob Madsen , Timothy J. Pennycook , Toma Susi

We introduce a new image cytometer design for detection of very small particulate and demonstrate its capability in water analysis. The device is a compact microscope composed of off the shelf components, such as a light emitting diode…

Optics · Physics 2016-02-17 Juan Miguel Pérez , Marc Jofre , Pedro Martínez , Valerio Pruneri
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