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Nonequilibrium experiments of single biomolecules such as force-induced unfolding reveal details about a few degrees of freedom of a complex system. Molecular dynamics simulations can provide complementary information, but exploration of…

Statistical Mechanics · Physics 2011-04-28 Alex Dickson , Mark Maienschein-Cline , Allison Tovo-Dwyer , Jeff R. Hammond , Aaron R. Dinner

Using coarse-grained model we have explored forced-unfolding of RNA hairpin as a function of $f_S$ and the loading rate ($r_f$). The simulations and theoretical analysis have been done without and with the handles that are explicitly…

Biomolecules · Quantitative Biology 2009-11-13 Changbong Hyeon , D. Thirumalai

In contrast to proteins much less attention has been focused on development of computational models for describing RNA molecules, which are being recognized as playing key roles in many cellular functions. Current atomically detailed force…

Biomolecules · Quantitative Biology 2014-02-28 Changbong Hyeon , Natalia A. Denesyuk , D. Thirumalai

We consider a simple model for the unfolding of RNA tertiary structure under dynamic loading. The opening of such a structure is regarded as a two step process, each corresponding to the overcoming of a single energy barrier. The resulting…

Biomolecules · Quantitative Biology 2009-11-10 Alberto Imparato , Luca Peliti

We present a panoramic view of the utility of coarse-grained (CG) models to study folding and functions of proteins and RNA. Drawing largely on the methods developed in our group over the last twenty years, we describe a number of key…

Biomolecules · Quantitative Biology 2008-08-25 D. L. Pincus , S. S. Cho , C. Hyeon , D. Thirumalai

We propose a new approach for modelling the process of RNA folding as a graph transformation guided by the global value of free energy. Since the folding process evolves towards a configuration in which the free energy is minimal, the…

Formal Languages and Automata Theory · Computer Science 2016-12-07 Adane Letta Mamuye , Emanuela Merelli , Luca Tesei

RNA binding proteins play a crucial role in post-transcriptional gene regulation by controlling the transport, processing, and translation of their target RNAs. Post-transcriptional gene regulation leads to the differential expression of…

Biomolecules · Quantitative Biology 2026-03-24 Danielle Wampler , Ralf Bundschuh

Gene expression consists in the synthesis of proteins from the information encoded on DNA. One of the two main steps of gene expression is the translation of messenger RNA (mRNA) into polypeptide sequences of amino acids. Here, by taking…

Biomolecular graph analysis has recently gained much attention in the emerging field of geometric deep learning. Here we focus on organizing biomolecular graphs in ways that expose meaningful relations and variations between them. We…

Machine Learning · Computer Science 2022-03-29 Egbert Castro , Andrew Benz , Alexander Tong , Guy Wolf , Smita Krishnaswamy

This contribution focuses on the fascinating RNA molecule, its sequence-dependent folding driven by base-pairing interactions, the interplay between these interactions and natural evolution, and its multiple regulatory roles. The four of us…

Statistical Mechanics · Physics 2022-07-28 Simona Cocco , Andrea De Martino , Andrea Pagnani , Martin Weigt

Single-molecule experiments provide new insights into biological processes hitherto not accessible by measurements performed on bulk systems. We report on a study of the kinetics of a triple-branch DNA molecule with four conformational…

Biological Physics · Physics 2011-06-13 Sandra Engel , Anna Alemany , Nuria Forns , Philipp Maass , Felix Ritort

Delays in protein synthesis cause a confounding effect when constructing Gene Regulatory Networks (GRNs) from RNA-sequencing time-series data. Accurate GRNs can be very insightful when modelling development, disease pathways, and drug…

Molecular Networks · Quantitative Biology 2020-10-07 Jacob Moss , Pietro Lió

RNA's diverse biological functions stem from its structural versatility, yet accurately predicting and designing RNA sequences given a 3D conformation (inverse folding) remains a challenge. Here, I introduce a deep learning framework that…

Quantitative Methods · Quantitative Biology 2026-01-06 Annabelle Yao

We summarize the recent simulation progress of micromanipulation experiments on RNAs. Our work mainly consults with two important small RNAs unfolding experiments carried out by Bustamante group. Our results show that, in contrast to…

Soft Condensed Matter · Physics 2007-05-23 Fei Liu , Zhong-can Ou-Yang

Motivation: Networks underlie the generation and interpretation of many biological datasets: gene networks shed light on the regulatory structure of the genome, and cell networks can capture structure of the tumor micro-environment.…

Machine Learning · Statistics 2026-03-18 Bailey Andrew , Erica L. Harris , James A. Poulter , David R. Westhead , Luisa Cutillo

RNA function is intimately related to its structural dynamics. Molecular dynamics simulations are useful for exploring biomolecular flexibility but are severely limited by the accessible timescale. Enhanced sampling methods allow this…

Biomolecules · Quantitative Biology 2018-02-06 Vojtěch Mlýnský , Giovanni Bussi

Extracting the intrinsic kinetic information of biological molecule from its single-molecule kinetic data is of considerable biophysical interest. In this work, we theoretically investigate the feasibility of inferring single RNA's…

Soft Condensed Matter · Physics 2007-10-05 Fei Liu , Zhong-can Ou-Yang

Elastic network models (ENMs) are valuable and efficient tools for characterizing the collective internal dynamics of proteins based on the knowledge of their native structures. The increasing evidence that the biological functionality of…

Biomolecules · Quantitative Biology 2015-09-01 Giovanni Pinamonti , Sandro Bottaro , Cristian Micheletti , Giovanni Bussi

Gaussian network model(GNM) and anisotropic network model(ANM) are some of the most popular methods for the study of protein flexibility and related functions. In this work, we propose generalized GNM(gGNM) and ANM methods and show that the…

Biomolecules · Quantitative Biology 2016-01-20 Kelin Xia , Kristopher Opron , Guo-Wei Wei

We present a simple kinetic model for the assembly of small single-stranded RNA viruses that can be used to carry out analytical packaging contests between different types of RNA molecules. The RNA selection mechanism is purely kinetic and…

Biological Physics · Physics 2021-02-09 Inbal Mizrahi , Robijn Bruinsma , Joseph Rudnick