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Multisite phosphorylation plays an important role in intracellular signaling. There has been much recent work aimed at understanding the dynamics of such systems when the phosphorylation/dephosphorylation mechanism is distributive, that is,…

Molecular Networks · Quantitative Biology 2014-10-23 Carsten Conradi , Anne Shiu

Multisite protein phosphorylation plays a pivotal role in regulating cellular signaling and decision-making processes. In this study, we focus on the mathematical underpinnings and informational aspects of sequential, distributive…

Molecular Networks · Quantitative Biology 2025-07-22 Iman Tavassoly , Adel Mehrpooya , Parsa Mirlohi , Zahra Abbaspourasadollah

Multistationarity, underlies biochemical switching and cellular decision-making. We study how multistationarity in the sequential n-site phosphorylation-dephosphorylation cycle is affected when only some species are open, meaning allowed to…

Dynamical Systems · Mathematics 2025-11-14 Praneet Nandan , Beatriz Pascual-Escudero , Diego Rojas La Luz

Post-transductional modifications tune the functions of proteins and regulate the collective dynamics of biochemical networks that determine how cells respond to environmental signals. For example, protein phosphorylation and nitrosylation…

Cell Behavior · Quantitative Biology 2009-11-13 Roberto Chignola , Chiara Dalla Pellegrina , Alessio Del Fabbro , Edoardo Milotti

Multisite phosphorylation networks are encountered in many intracellular processes like signal transduction, cell-cycle control or nuclear signal integration. In this contribution networks describing the phosphorylation and…

Molecular Networks · Quantitative Biology 2013-07-03 Katharina Holstein , Dietrich Flockerzi , Carsten Conradi

Oscillations play a major role in a number of biological systems, from predator-prey models of ecology to circadian clocks. In this paper we focus on the question of whether oscillations exist within dual-site phosphorylation systems.…

Molecular Networks · Quantitative Biology 2020-08-03 H. Tung

The utilization of multiple phosphorylation sites in regulating a biological response is ubiquitous in cell signaling. If each site contributes an additional, equivalent binding site, then one consequence of an increase in the number of…

Subcellular Processes · Quantitative Biology 2009-11-13 Jason W. Locasale

We introduce a general framework for biological systems, called MESSI systems, that describe Modifications of type Enzyme-Substrate or Swap with Intermediates, and we prove general results based on the network structure. Many…

Molecular Networks · Quantitative Biology 2018-05-08 Mercedes Pérez Millán , Alicia Dickenstein

Phosphorylation networks, representing the mechanisms by which proteins are phosphorylated at one or multiple sites, are ubiquitous in cell signalling and display rich dynamics such as unlimited multistability. Dual-site phosphorylation…

Dynamical Systems · Mathematics 2024-05-28 Elisenda Feliu , Nidhi Kaihnsa

Ordered distributive double phosphorylation is a recurrent motif in intracellular signaling and control. It is either sequential (where the site phosphorylated last is dephosphorylated first) or cyclic (where the site phosphorylated first…

Molecular Networks · Quantitative Biology 2024-02-09 Carsten Conradi , Maya Mincheva

Protein functions in cells may be activated or modified by the attachment of several kinds of chemical groups. While protein phosphorylation, i.e. the attachment of a phosphoryl (PO$_3^-$) group, is the most studied form of protein…

Biological Physics · Physics 2015-06-26 Edoardo Milotti , Alessio Del Fabbro , Chiara Dalla Pellegrina , Roberto Chignola

The dual phosphorylation network provides an essential component of intracellular signaling, affecting the expression of phenotypes and cell metabolism. For particular choices of kinetic parameters, this system exhibits multistationarity, a…

Optimization and Control · Mathematics 2024-09-25 May Cai , Matthias Himmelmann , Birte Ostermann

The parameter region of multistationarity of a reaction network contains all the parameters for which the associated dynamical system exhibits multiple steady states. Describing this region is challenging and remains an active area of…

Molecular Networks · Quantitative Biology 2024-10-11 Nidhi Kaihnsa , Máté L. Telek

Multisite protein phosphorylation plays a prominent role in intracellular processes like signal transduction, cell-cycle control and nuclear signal integration. Many proteins are phosphorylated in a sequential and distributive way at more…

Molecular Networks · Quantitative Biology 2014-04-24 Dietrich Flockerzi , Katharina Holstein , Carsten Conradi

The MAP kinase cascade is a network of enzymatic reactions arranged in layers. In each layer occurs a multiple futile cycle of phosphorylations. The fully phosphorylated substrate then serves as an enzyme for the layer below. This papers…

Molecular Networks · Quantitative Biology 2015-09-10 Juliette Hell , Alan D. Rendall

Protein nanoclustering is a characteristic feature of their activated state and is essential for forming numerous subcellular structures. The formation of these nanoclusters is highly dependent on a series of post-translational…

Biological Physics · Physics 2025-11-13 Olivier Destaing , Bertrand Fourcade

We apply tools from real algebraic geometry to the problem of multistationarity of chemical reaction networks. A particular focus is on the case of reaction networks whose steady states admit a monomial parametrization. For such systems we…

Molecular Networks · Quantitative Biology 2019-08-14 Carsten Conradi , Alexandru Iosif , Thomas Kahle

We study the ultrasensitivity of multisite binding processes where ligand molecules can bind to several binding sites, considering more particularly recent models involving complex chemical reactions in phosphorylation systems such as…

Subcellular Processes · Quantitative Biology 2016-05-02 Michaël Dougoud , Christian Mazza , Laura Vinckenbosch

This work investigates the emergence of oscillations in one of the simplest cellular signaling networks exhibiting oscillations, namely, the dual-site phosphorylation and dephosphorylation network (futile cycle), in which the mechanism for…

Molecular Networks · Quantitative Biology 2019-02-05 Carsten Conradi , Maya Mincheva , Anne Shiu

Protein phosphorylation cycles are important mechanisms of the post translational modification of a protein and as such an integral part of intracellular signaling and control. We consider the sequential phosphorylation and…

Molecular Networks · Quantitative Biology 2019-11-06 Carsten Conradi , Elisenda Feliu , Maya Mincheva
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