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A major challenge in next-generation genome sequencing (NGS) is to assemble massive overlapping short reads that are randomly sampled from DNA fragments. To complete assembling, one needs to finish a fundamental task in many leading…

Genomics · Quantitative Biology 2015-05-26 Yang Li , XifengYan

The third-generation long reads sequencing technologies, such as PacBio and Nanopore, have great advantages over second-generation Illumina sequencing in de novo assembly studies. However, due to the inherent low base accuracy,…

Genomics · Quantitative Biology 2020-03-27 Hengchao Wang , Bo Liu , Yan Zhang , Fan Jiang , Yuwei Ren , Lijuan Yin , Hangwei Liu , Sen Wang , Wei Fan

Motivation: Building the histogram of occurrences of every $k$-symbol long substring of nucleotide data is a standard step in many bioinformatics applications, known under the name of $k$-mer counting. Its applications include developing de…

Data Structures and Algorithms · Computer Science 2017-03-03 Sebastian Deorowicz , Marek Kokot , Szymon Grabowski , Agnieszka Debudaj-Grabysz

The emergence of Next Generation Sequencing (NGS) platforms has increased the throughput of genomic sequencing and in turn the amount of data that needs to be processed, requiring highly efficient computation for its analysis. In this…

Distributed, Parallel, and Cluster Computing · Computer Science 2017-12-12 Nicola Cadenelli , Jorda Polo , David Carrera

In generating large quantities of DNA data, high-throughput sequencing technologies require advanced bioinformatics infrastructures for efficient data analysis. k-mer counting, the process of quantifying the frequency of fixed-length k DNA…

Distributed, Parallel, and Cluster Computing · Computer Science 2024-07-11 Yifan Li , Giulia Guidi

k-mers (nucleotide strings of length k) form the basis of several algorithms in computational genomics. In particular, k-mer abundance information in sequence data is useful in read error correction, parameter estimation for genome…

Data Structures and Algorithms · Computer Science 2016-09-20 Naveen Sivadasan , Rajgopal Srinivasan , Kshama Goyal

Background: Short sequence substrings of a fixed length k, called k-mers, are a ubiquitous computational primitive in bioinformatics, used across sequence indexing, read mapping, genome assembly, metagenomic classification, and comparative…

Genomics · Quantitative Biology 2026-05-15 Lucas Czech

String Kernel (SK) techniques, especially those using gapped $k$-mers as features (gk), have obtained great success in classifying sequences like DNA, protein, and text. However, the state-of-the-art gk-SK runs extremely slow when we…

Machine Learning · Computer Science 2017-09-19 Ritambhara Singh , Arshdeep Sekhon , Kamran Kowsari , Jack Lanchantin , Beilun Wang , Yanjun Qi

The wide array of currently available genomes display a wonderful diversity in size, composition and structure with many more to come thanks to several global biodiversity genomics initiatives starting in recent years. However, sequencing…

K-mer counting is a requisite process for DNA assembly because it speeds up its overall process. The frequency of K-mers is used for estimating the parameters of DNA assembly, error correction, etc. The process also provides a list of…

Databases · Computer Science 2023-05-15 Sabuzima Nayak , Ripon Patgiri

This paper describes a new asynchronous algorithm and implementation for the problem of k-mer counting (KC), which concerns quantifying the frequency of length k substrings in a DNA sequence. This operation is common to many computational…

Distributed, Parallel, and Cluster Computing · Computer Science 2025-05-08 Souvadra Hati , Akihiro Hayashi , Richard Vuduc

The extraction of $k$-mers is a fundamental component in many complex analyses of large next-generation sequencing datasets, including reads classification in genomics and the characterization of RNA-seq datasets. The extraction of all…

Quantitative Methods · Quantitative Biology 2021-01-19 Diego Santoro , Leonardo Pellegrina , Fabio Vandin

Counting the frequencies of k-mers in read libraries is often a first step in the analysis of high-throughput sequencing experiments. Infrequent k-mers are assumed to be a result of sequencing errors. The frequent k-mers constitute a…

Genomics · Quantitative Biology 2013-05-09 Rajat Shuvro Roy , Debashish Bhattacharya , Alexander Schliep

Motivation: Next-generation sequencing tools have enabled producing of huge amount of genomic information at low cost. Unfortunately, presence of sequencing errors in such data affects quality of downstream analyzes. Accuracy of them can be…

Genomics · Quantitative Biology 2017-03-03 Maciej Dlugosz , Sebastian Deorowicz

K-mer abundance analysis is widely used for many purposes in nucleotide sequence analysis, including data preprocessing for de novo assembly, repeat detection, and sequencing coverage estimation. We present the khmer software package for…

Genomics · Quantitative Biology 2014-08-07 Qingpeng Zhang , Jason Pell , Rosangela Canino-Koning , Adina Chuang Howe , C. Titus Brown

This paper provides a comprehensive survey of data structures for representing k-mer sets, which are fundamental in high-throughput sequencing analysis. It categorizes the methods into two main strategies: those using fingerprinting and…

Genomics · Quantitative Biology 2024-09-13 Camille Marchet

Background: With the fast development of next generation sequencing technologies, increasing numbers of genomes are being de novo sequenced and assembled. However, most are in fragmental and incomplete draft status, and thus it is often…

Genomics · Quantitative Biology 2020-02-28 Binghang Liu , Yujian Shi , Jianying Yuan , Xuesong Hu , Hao Zhang , Nan Li , Zhenyu Li , Yanxiang Chen , Desheng Mu , Wei Fan

The task of understanding and interpreting the complex information encoded within genomic sequences remains a grand challenge in biological research and clinical applications. In this context, recent advancements in large language model…

Genomics · Quantitative Biology 2024-09-25 Qihang Zhao , Chi Zhang , Weixiong Zhang

Motivation: With the rapid expansion of large-scale biological datasets, DNA and protein sequence alignments have become essential for comparative genomics and proteomics. These alignments facilitate the exploration of sequence similarity…

Genomics · Quantitative Biology 2024-12-02 Michail Patsakis , Kimonas Provatas , Ioannis Mouratidis , Ilias Georgakopoulos-Soares

Obtaining effective representations of DNA sequences is crucial for genome analysis. Metagenomic binning, for instance, relies on genome representations to cluster complex mixtures of DNA fragments from biological samples with the aim of…

Machine Learning · Computer Science 2024-11-05 Abdulkadir Celikkanat , Andres R. Masegosa , Thomas D. Nielsen
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