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The identification of metabolites from complex biological samples often involves matching experimental mass spectrometry data to signatures of compounds derived from massive chemical databases. However, misidentifications may result due to…

Quantitative Methods · Quantitative Biology 2023-07-11 Christine H. Chang , Bryan J. Killinger , Ryan S. Renslow , Sean M. Colby

Chemical formula annotation for tandem mass spectrometry (MS/MS) data is the first step toward structurally elucidating unknown metabolites. While great strides have been made toward solving this problem, the current state-of-the-art method…

Quantitative Methods · Quantitative Biology 2023-10-24 Samuel Goldman , Jiayi Xin , Joules Provenzano , Connor W. Coley

Background: The study of genome-scale metabolic models and their underlying networks is one of the most important fields in systems biology. The complexity of these models and their description makes the use of computational tools an…

Molecular Networks · Quantitative Biology 2012-12-03 D. Gamermann , A. Montagud , R. A. Jaime Infante , J. Triana , P. F. de Córdoba , J. F. Urchueguía

Metabolites, small molecules that are involved in cellular reactions, provide a direct functional signature of cellular state. Untargeted metabolomics experiments usually relies on tandem mass spectrometry to identify the thousands of…

Quantitative Methods · Quantitative Biology 2015-01-29 Kai Dührkop , Sebastian Böcker

Metabolomics complements investigation of the genome, transcriptome, and proteome of an organism. Today, the vast majority of metabolites remain unknown, in particular for non-model organisms. Mass spectrometry is one of the predominant…

Quantitative Methods · Quantitative Biology 2013-07-31 Kai Dührkop , Marcus Ludwig , Marvin Meusel , Sebastian Böcker

High throughput metabolomics data are fraught with both non-ignorable missing observations and unobserved factors that influence a metabolite's measured concentration, and it is well known that ignoring either of these complications can…

Methodology · Statistics 2019-09-09 Chris McKennan , Carole Ober , Dan Nicolae

Compound identification and structure annotation from mass spectra is a well-established task widely applied in drug detection, criminal forensics, small molecule biomarker discovery and chemical engineering. We propose SpecTUS: Spectral…

Machine Learning · Computer Science 2026-02-23 Adam Hájek , Michal Starý , Elliott Price , Filip Jozefov , Helge Hecht , Aleš Křenek

Untargeted metabolomics based on liquid chromatography-mass spectrometry technology is quickly gaining widespread application given its ability to depict the global metabolic pattern in biological samples. However, the data is noisy and…

Methodology · Statistics 2026-03-24 Guoxuan Ma , Jian Kang , Tianwei Yu

Electrospray tandem mass spectrometry (ESI-MS/MS) is commonly used in high throughput metabolomics. One of the key obstacles to the effective use of this technology is the difficulty in interpreting measured spectra to accurately and…

Computational Engineering, Finance, and Science · Computer Science 2014-06-10 F. Allen , R. Greiner , D. Wishart

Many diseases cause significant changes to the concentrations of small molecules (aka metabolites) that appear in a person's biofluids, which means such diseases can often be readily detected from a person's "metabolic profile". This…

A key challenge in metabolomics is annotating measured spectra from a biological sample with chemical identities. Currently, only a small fraction of measurements can be assigned identities. Two complementary computational approaches have…

Machine Learning · Computer Science 2022-03-28 Xinmeng Li , Hao Zhu , Li-ping Liu , Soha Hassoun

The diverse metabolic pathways are fundamental to all living organisms, as they harvest energy, synthesize biomass components, produce molecules to interact with the microenvironment, and neutralize toxins. While discovery of new…

Biomolecules · Quantitative Biology 2022-11-30 Thiru Siddharth , Nathan Lewis

Motivation: A major challenge in metabolomics is annotation: assigning molecular structures to mass spectral fragmentation patterns. Despite recent advances in molecule-to-spectra and in spectra-to-molecular fingerprint prediction (FP),…

Quantitative Methods · Quantitative Biology 2025-06-10 Apurva Kalia , Yan Zhou Chen , Dilip Krishnan , Soha Hassoun

Identifying the interaction targets of bioactive compounds is a foundational element for deciphering their pharmacological effects. Target prediction algorithms equip researchers with an effective tool to rapidly scope and explore potential…

Machine Learning · Computer Science 2025-01-03 Haojie Wang , Zhe Zhang , Haotian Gao , Xiangying Zhang , Jingyuan Li , Zhihang Chen , Xinchong Chen , Yifei Qi , Yan Li , Renxiao Wang

Motivation: NMR spectra are widely used in metabolomics to obtain metabolite profiles in complex biological mixtures. Common methods used to assign and estimate concentrations of metabolites involve either an expert manual peak fitting or…

Applications · Statistics 2012-05-30 Jie Hao , William Astle , Maria De Iorio , Timothy Ebbels

Background. Emerging technologies now allow for mass spectrometry based profiling of up to thousands of small molecule metabolites (metabolomics) in an increasing number of biosamples. While offering great promise for revealing insight into…

Liquid chromatography mass spectrometry (LC-MS)-based metabolomics and exposomics aim to measure detectable small molecules in biological samples. The results facilitate hypothesis-generating discovery of metabolic changes and disease…

Quantitative Methods · Quantitative Biology 2026-01-06 Xujun Che , Xiuxia Du , Depeng Xu

Cancer is a heterogeneous disease with different combinations of genetic and epigenetic alterations driving the development of cancer in different individuals. While these alterations are believed to converge on genes in key cellular…

Quantitative Methods · Quantitative Biology 2015-03-31 Mark D. M. Leiserson , Hsin-Ta Wu , Fabio Vandin , Benjamin J. Raphael

Untargeted metabolomics generates large volumes of tandem mass spectrometry (MS/MS) data and computational annotations that can reveal molecular mechanisms across organisms and environments. Public reuse has improved through harmonized…

Databases · Computer Science 2026-05-26 Matthieu Féraud , Dina Boukhajou , Fabien Gandon , Louis-Félix Nothias

The discovery and identification of molecules in biological and environmental samples is crucial for advancing biomedical and chemical sciences. Tandem mass spectrometry (MS/MS) is the leading technique for high-throughput elucidation of…

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