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I present a review of the theoretical and computational methodologies that have been used to model the assembly of viral capsids. I discuss the capabilities and limitations of approaches ranging from equilibrium continuum theories to…

Biomolecules · Quantitative Biology 2013-01-09 Michael F. Hagan

A series of simulations aimed at elucidating the self-assembly dynamics of spherical virus capsids is described. This little-understood phenomenon is a fascinating example of the complex processes that occur in the simplest of organisms.…

Soft Condensed Matter · Physics 2015-05-19 D. C. Rapaport

The packaging of genetic material within a protein shell, called the capsid, marks a pivotal step in the life cycle of numerous single-stranded RNA viruses. Understanding how hundreds, or even thousands, of proteins assemble around the…

Biological Physics · Physics 2024-09-04 Siyu Li , Guillaume Tresset , Roya Zandi

Viruses are nanoscale entities containing a nucleic acid genome encased in a protein shell called a capsid, and in some cases surrounded by a lipid bilayer membrane. This review summarizes the physics that govern the processes by which…

Biomolecules · Quantitative Biology 2015-05-20 Jason D Perlmutter , Michael F Hagan

We consider self-assembly of proteins into a virus capsid by the methods of molecular dynamics. The capsid corresponds either to SPMV or CCMV and is studied with and without the RNA molecule inside. The proteins are flexible and described…

Biomolecules · Quantitative Biology 2018-11-05 Karol Wolek , Marek Cieplak

We develop coarse-grained models that describe the dynamic encapsidation of functionalized nanoparticles by viral capsid proteins. We find that some forms of cooperative interactions between protein subunits and nanoparticles can…

Biomolecules · Quantitative Biology 2009-11-13 Michael F. Hagan

In many viruses, hundreds of proteins assemble an outer shell (capsid) around the viral nucleic acid to form an infectious virion. How the assembly process selects the viral genome amidst a vast excess of diverse cellular nucleic acids is…

Soft Condensed Matter · Physics 2025-08-26 Layne B. Frechette , Michael F. Hagan

Results from molecular dynamics simulations of simple, structured particles capable of self-assembling into polyhedral shells are described. The analysis focuses on the growth histories of individual shells in the presence of an explicit…

Soft Condensed Matter · Physics 2010-03-01 D. C. Rapaport

The formation of a viral capsid -- the highly-ordered protein shell that surrounds the genome of a virus -- is the canonical example of self-assembly. The capsids of many positive-sense RNA viruses spontaneously assemble from in vitro…

Soft Condensed Matter · Physics 2022-06-08 Rees F. Garmann , Aaron M. Goldfain , Vinothan N. Manoharan

During the lifecycle of many single-stranded RNA viruses, including many human pathogens, a protein shell called the capsid spontaneously assembles around the viral genome. Understanding the mechanisms by which capsid proteins selectively…

Biomolecules · Quantitative Biology 2015-02-19 J. D. Perlmutter , M. F. Hagan

Realistic evolutionary fitness landscapes are notoriously difficult to construct. A recent cutting-edge model of virus assembly consists of a dodecahedral capsid with $12$ corresponding packaging signals in three affinity bands. This whole…

Biomolecules · Quantitative Biology 2021-06-22 Pierre-Philippe Dechant , Yang-Hui He

Understanding protein self-assembly is important for many biological and industrial processes. Proteins can self-assemble into crystals, filaments, gels, and other amorphous aggregates. The final forms include virus capsids and condensed…

Soft Condensed Matter · Physics 2016-04-15 Jennifer J. McManus , Patrick Charbonneau , Emanuela Zaccarelli , Neer Asherie

Self-assembly at submicroscopic scales is an important but little understood phenomenon. A prominent example is virus capsid growth, whose underlying behavior can be modeled using simple particles that assemble into polyhedral shells.…

Soft Condensed Matter · Physics 2008-10-28 D. C. Rapaport

Capsids of many viruses assemble around nucleic acids or other polymers. Understanding how the properties of the packaged polymer affect the assembly process could promote biomedical efforts to prevent viral assembly or nanomaterials…

Biomolecules · Quantitative Biology 2015-05-18 Aleksandr Kivenson , Michael F. Hagan

We use computer simulations to study a model, first proposed by Wales [1], for the reversible and monodisperse self-assembly of simple icosahedral virus capsid structures. The success and efficiency of assembly as a function of…

Biomolecules · Quantitative Biology 2010-02-24 Iain G. Johnston , Ard A. Louis , Jonathan P. K. Doye

Understanding the pathways by which viral capsid proteins assemble around their genomes could identify key intermediates as potential drug targets. In this work we use computer simulations to characterize assembly over a wide range of…

Biomolecules · Quantitative Biology 2014-05-15 Jason D Perlmutter , Matthew R Perkett , Michael F Hagan

Simple RNA viruses self-assemble spontaneously and encapsulate their genome into a shell called the capsid. This process is mainly driven by the attractive electrostatic interaction between the positive charges on capsid proteins and the…

Biological Physics · Physics 2022-02-22 Sanaz Panahandeh , Siyu Li , Bogdan Dragnea , Roya Zandi

We develop a class of models with which we simulate the assembly of particles into T1 capsid-like objects using Newtonian dynamics. By simulating assembly for many different values of system parameters, we vary the forces that drive…

Biomolecules · Quantitative Biology 2009-11-11 Michael F. Hagan , David Chandler

Understanding how virus capsids assemble around their nucleic acid (NA) genomes could promote efforts to block viral propagation or to reengineer capsids for gene therapy applications. We develop a coarse-grained model of capsid proteins…

Biomolecules · Quantitative Biology 2014-05-15 Jason D. Perlmutter , Cong Qiao , Michael F. Hagan

Molecular simulations of the self-assembly of cone-shaped particles with specific, attractive interactions are performed. Upon cooling from random initial conditions, we find that the cones self assemble into clusters and that clusters…

Materials Science · Physics 2009-11-11 Ting Chen , Zhenli Zhang , Sharon C. Glotzer
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