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Several processes in the cell, such as gene regulation, start when key proteins recognise and bind to short DNA sequences. However, as these sequences can be hundreds of million times shorter than the genome, they are hard to find by simple…

Subcellular Processes · Quantitative Biology 2021-01-27 Markus Nyberg , Tobias Ambjörnsson , Per Stenberg , and Ludvig Lizana

In combining DNA nanotechnology and high-bandwidth single-molecule detection in nanopipettes, we demonstrate an all-electric, label-free hybridisation sensor for short DNA sequences (< 100 nt). Such short fragments are known to occur as…

The denaturation of the double helix is a template for fundamental biological functions such as replication and transcription involving the formation of local fluctuational openings. The denaturation transition is studied for heterogeneous…

Biomolecules · Quantitative Biology 2014-06-03 Marco Zoli

DNA helicases are molecular motors that use the energy from ATP hydrolysis to move along DNA, promoting the unwinding or rewinding of the double helix. Here, we use magnetic and optical tweezers to track the motion of three helicases, gp41,…

Biological Physics · Physics 2025-03-20 V. Rodriguez-Franco , M. M. Spiering , F. Ritort , M. Mañosas

The recA/RAD51 gene family encodes a diverse set of recombinase proteins that effect homologous recombination, DNA-repair, and genome stability. The recA gene family is expressed in almost all species of Eubacteria, Archaea, and Eukaryotes,…

Recent results from the ATLAS and the CMS experiments at the Large Hadron Collider indicate the presence of a top-quark pair bound state near the threshold region. We present a way to reconstruct a toponium state at the $t\bar{t}$ threshold…

High Energy Physics - Phenomenology · Physics 2026-04-01 Aman Desai , Amelia Lovison , Paul Jackson

Understanding the relationship between protein sequences and their functions is fundamental to protein engineering, but this task is hindered by the combinatorially vast sequence space and the experimental noise inherent in fitness…

Quantitative Methods · Quantitative Biology 2025-06-23 Muhammad Daud , Philippe Charton , Cedric Damour , Jingbo Wang , Frederic Cadet

Most proteins involved in processing DNA accomplish their activities as a monomer or as a component of a multimer containing a relatively small number of other elements. They generally act locally, binding to one or a few small regions of…

Biomolecules · Quantitative Biology 2009-11-10 Kevin Klapstein , Tom Chou , Robijn Bruinsma

We study numerically the mechanical stability and elasticity properties of duplex DNA molecules within the frame of a network model incorporating microscopic degrees of freedom related with the arrangement of the base pairs. We pay special…

Pattern Formation and Solitons · Physics 2015-06-26 D. Hennig , J. F. R. Archilla

Gene regulation is one of the most important fundamental biological processes in living cells. It involves multiple protein molecules that locate specific sites on DNA and assemble gene initiation or gene repression multi-molecular…

Biological Physics · Physics 2019-10-23 Jaeoh Shin , Anatoly B. Kolomeisky

The protein recombinase can change the knot type of circular DNA. The action of a recombinase converting one knot into another knot is normally mathematically modeled by band surgery. Band surgeries on a 2-bridge knot N((4mn-1)/(2m))…

Geometric Topology · Mathematics 2016-01-20 Isabel K. Darcy , Kai Ishihara , Ram K. Medikonduri , Koya Shimokawa

Proteins are the active working horses in our body. These biomolecules perform all vital cellular functions from DNA replication and general biosynthesis to metabolic signaling and environmental sensing. While static 3D structures are now…

Quantitative Methods · Quantitative Biology 2020-10-16 Sonja Schmid , Cees Dekker

Genetic recombination can produce heterogeneous phylogenetic histories within a set of homologous genes. Delineating recombination events is important in the study of molecular evolution, as inference of such events provides a clearer…

Populations and Evolution · Quantitative Biology 2007-09-13 Cheong Xin Chan , Robert G. Beiko , Mark A. Ragan

In this project, we present a deep neural network (DNN)-based biophysics model that uses multi-scale and uniform topological and electrostatic features to predict protein properties, such as Coulomb energies or solvation energies. The…

Machine Learning · Computer Science 2026-03-16 Elyssa Sliheet , Md Abu Talha , Weihua Geng

Genomic foundation models have the potential to decode DNA syntax, yet face a fundamental tradeoff in their input representation. Standard fixed-vocabulary tokenizers fragment biologically meaningful motifs such as codons and regulatory…

Replication and transcription are two important processes in living systems. To execute such processes, various proteins work far away from equilibrium in a staggered way. Motivated by this, aspects of hysteresis during unzipping of DNA…

Soft Condensed Matter · Physics 2013-03-14 Garima Mishra , Poulomi Sadhukhan , Somendra M Bhattacharjee , Sanjay Kumar

In recent years, significant progress in understanding the properties of supercoiled DNA has been obtained due to nanotechniques that made stretching and twisting of single molecules possible. Quantitative interpretation of such experiments…

Biomolecules · Quantitative Biology 2015-06-05 Alexey K. Mazur

Frameshift mutations in protein-coding DNA sequences produce a drastic change in the resulting protein sequence, which prevents classic protein alignment methods from revealing the proteins' common origin. Moreover, when a large number of…

Quantitative Methods · Quantitative Biology 2011-01-18 Marta L. Gîrdea , Laurent Noé , Gregory Kucherov

DNA code design aims to generate a set of DNA sequences (codewords) with minimum likelihood of undesired hybridizations among sequences and their reverse-complement (RC) pairs (cross-hybridization). Inspired by the distinct hybridization…

Information Theory · Computer Science 2020-05-15 Yixin Wang , Md Noor-A-Rahim , Erry Gunawan , Yong Liang Guan , Chueh Loo Poh

Protein-DNA interactions are vital for many processes in living cells, especially transcriptional regulation and DNA modification. To further our understanding of these important processes on the microscopic level, it is necessary that…

Biomolecules · Quantitative Biology 2007-05-23 Jason E Donald , William W Chen , Eugene I Shakhnovich