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Molecular carriers represent an increasingly common strategy in the field of nanopore sensing to use secondary molecules to selectively report on the presence of target analytes in solution, allowing for sensitive assays of otherwise…

Biomolecules · Quantitative Biology 2023-05-10 Zachary Roelen , Vincent Tabard-Cossa

Bacteriophages infect cells by attaching to the outer membrane and injecting their DNA into the cell.The phage DNA is then transcribed by the cell's transcription machinery.A number of physical mechanisms by which DNA can be translocated…

Biological Physics · Physics 2013-01-10 Sandip Ghosal

Collective coordinate and direct numerical integration methods are applied to the analysis of a one-dimensional DNA model. A modification of the coupling constant in an extended region is found to be less selective towards the breather it…

Condensed Matter · Physics 2009-10-28 Julian J. -L. Ting , Michel Peyrard

In this work, we present an automated platform for trapping and stretching individual micro- and nanoscale objects in solution using electrokinetic forces. The platform can trap objects at the stagnation point of a planar elongational…

Instrumentation and Detectors · Physics 2023-04-24 Beatrice W. Soh , Zi-En Ooi , Eleonore Vissol-Gaudin , Chang Jie Leong , Kedar Hippalgaonkar

We report in detail our experiments on the conduction of $\lambda$ DNA molecules over a wide range of temperature deposited across slits in a few nanometers thick platinum film. These insulating slits were fabricated using focused ion beam…

Mesoscale and Nanoscale Physics · Physics 2015-06-11 A. D. Chepelianskii , D. Klinov , A. Kasumov , S. Guéron , O. Pietrement , S. Lyonnais , H. Bouchiat

DNA is now firmly established as a versatile and robust platform for achieving synthetic nanostructures. While the folding of single molecules into complex structures is routinely achieved through engineering basepair sequences, much less…

Soft Condensed Matter · Physics 2025-05-15 Soumen De Karmakar , Thomas Speck

We have investigated the conduction over a wide range of temperature of $\lambda$ DNA molecules deposited across slits etched through a few nanometers thick platinum film. The slits are insulating before DNA deposition but contain metallic…

Mesoscale and Nanoscale Physics · Physics 2015-05-20 A. D. Chepelianskii , D. Klinov , A. Kasumov , S. Guéron , O. Pietrement , S. Lyonnais , H. Bouchiat

Technologies for sequencing (reading) and synthesizing (writing) DNA have progressed on a Moore's law-like trajectory over the last three decades. This has motivated the idea of using DNA for data storage. Theoretically, DNA-based storage…

Emerging Technologies · Computer Science 2023-07-07 Ajay Manicka , Andrew Stephan , Sriram Chari , Gemma Mendonsa , Peyton Okubo , John Stolzberg-Schray , Anil Reddy , Marc Riedel

We present Brownian dynamics simulations of the facilitated diffusion of a protein, modelled as a sphere with a binding site on its surface, along DNA, modelled as a semi-flexible polymer. We consider both the effect of DNA organisation in…

Biological Physics · Physics 2012-12-10 C. A. Brackley , M. E. Cates , D. Marenduzzo

Solid-state nanopores are emerging platforms for single-molecule protein sequencing due to their tolerance to hash physiology environment and compatibility with different electrical and optical detection methods. However, they suffer from…

Applied Physics · Physics 2024-01-05 Yingqi Zhao , Aliaksandr Hubarevich , Jian-An Huang , Francesco De Angelis

Grafting linkers with open ends of complementary single-stranded DNA makes a flexible tool to tune interactions between colloids,which facilitates the design of complex self-assembly structures. Recently, it has been proposed to coat…

Soft Condensed Matter · Physics 2018-01-31 Hao Hu , Pablo Sampedro Ruiz , Ran Ni

Primary human cells offer the most faithful representation of native human physiology, yet their practical utility is constrained by the difficulty of introducing exogenous genetic material. Electroporation provides a promising non-viral…

Applied Physics · Physics 2026-04-01 Hyun Woo Sung , Soojung Claire Hur

Electrokinetic transport phenomena can strongly influence the behaviour of macromolecules and colloidal particles in solution, with applications in, e.g., DNA translocation through nanopores, electro-osmotic flow in nanocapillaries, and…

Soft Condensed Matter · Physics 2016-08-03 Georg Rempfer , Gary B. Davies , Christian Holm , Joost de Graaf

Charge migration along DNA molecules has attracted scientific interest for over half a century. Reports on possible high rates of charge transfer between donor and acceptor through the DNA, obtained in the last decade from solution…

Materials Science · Physics 2009-11-10 Danny Porath , Gianaurelio Cuniberti , Rosa Di Felice

Cells operate in part by compartmentalizing chemical reactions. For example, recent work has shown that chromatin, the material that contains the cell's genome, can auto-regulate its structure by utilizing reaction products (proteins, RNA)…

Soft Condensed Matter · Physics 2024-10-30 Sam Wilken , Gabrielle R. Abraham , Omar A. Saleh

The interaction between nano- or micro-sized particles and cell membranes is of crucial importance in many biological and biomedical applications such as drug and gene delivery to cells and tissues. During their cellular uptake, the…

The dynamics of polymer translocation through a pore has been the subject of recent theoretical and experimental works. We have considered theoretical estimates and performed computer simulations to understand the mechanism of DNA uptake…

Soft Condensed Matter · Physics 2009-11-07 Zeno Farkas , Imre Derenyi , Tamas Vicsek

A purely electrical sensing scheme is presented that determines the concentration of macromolecules in solution by measuring the capacitance between planar microelectrodes. Concentrations of DNA in the ng/mL range have been used in samples…

Biological Physics · Physics 2009-01-20 Anja Henning , Joerg Henkel , Frank F Bier , Ralph Hoelzel

We study the trapping of charged particles and macromolecules (such as DNA) in salt gradients in aqueous solutions. The source for the salt gradient can be as simple as a dissolving ionic crystal, as shown by McDermott et al. [Langmuir 28,…

Soft Condensed Matter · Physics 2025-12-09 Richard P. Sear , Patrick B. Warren

In this work, we show that by varying the experimental conditions, the driving amplitude, a dynamic force microscope allows DNA properties to be selectively imaged. The substrate on which the DNA is fixed is a silica surface grafted with…

Biological Physics · Physics 2016-08-16 Laurent Nony , Rodolphe Boisgard , Jean-Pierre Aimé