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Reconstructing neuronal circuits at the level of synapses is a central problem in neuroscience and becoming a focus of the emerging field of connectomics. To date, electron microscopy (EM) is the most proven technique for identifying and…

Quantitative Methods · Quantitative Biology 2014-12-05 Stephen M. Plaza , Toufiq Parag , Gary B. Huang , Donald J. Olbris , Mathew A. Saunders , Patricia K. Rivlin

Connectomics is an emerging field in neuroscience that aims to reconstruct the 3-dimensional morphology of neurons from electron microscopy (EM) images. Recent studies have successfully demonstrated the use of convolutional neural networks…

Computer Vision and Pattern Recognition · Computer Science 2017-02-27 Shibani Santurkar , David Budden , Alexander Matveev , Heather Berlin , Hayk Saribekyan , Yaron Meirovitch , Nir Shavit

Synaptic connectivity detection is a critical task for neural reconstruction from Electron Microscopy (EM) data. Most of the existing algorithms for synapse detection do not identify the cleft location and direction of connectivity…

Computer Vision and Pattern Recognition · Computer Science 2018-10-26 Toufiq Parag , Daniel Berger , Lee Kamentsky , Benedikt Staffler , Donglai Wei , Moritz Helmstaedter , Jeff W. Lichtman , Hanspeter Pfister

The emerging field of connectomics aims to unlock the mysteries of the brain by understanding the connectivity between neurons. To map this connectivity, we acquire thousands of electron microscopy (EM) images with nanometer-scale…

Quantitative Methods · Quantitative Biology 2016-04-04 Stephen M. Plaza , Stuart E. Berg

High-throughput electron microscopy allows recording of lar- ge stacks of neural tissue with sufficient resolution to extract the wiring diagram of the underlying neural network. Current efforts to automate this process focus mainly on the…

Computer Vision and Pattern Recognition · Computer Science 2018-07-17 Julia Buhmann , Renate Krause , Rodrigo Ceballos Lentini , Nils Eckstein , Matthew Cook , Srinivas Turaga , Jan Funke

Producing connectomes from electron microscopy (EM) images has historically required a great deal of human proofreading effort. This manual annotation cost is the current bottleneck in scaling EM connectomics, for example, in making larger…

Computer Vision and Pattern Recognition · Computer Science 2025-10-01 Gary B Huang , William M Katz , Stuart Berg , Louis Scheffer

The promise of extracting connectomes and performing useful analysis on large electron microscopy (EM) datasets has been an elusive dream for many years. Tracing in even the smallest portions of neuropil requires copious human annotation,…

Reconstructing a map of neuronal connectivity is a critical challenge in contemporary neuroscience. Recent advances in high-throughput serial section electron microscopy (EM) have produced massive 3D image volumes of nanoscale brain tissue…

Morphology based analysis of cell types has been an area of great interest to the neuroscience community for several decades. Recently, high resolution electron microscopy (EM) datasets of the mouse brain have opened up opportunities for…

Developing automated and semi-automated solutions for reconstructing wiring diagrams of the brain from electron micrographs is important for advancing the field of connectomics. While the ultimate goal is to generate a graph of neuron…

Computer Vision and Pattern Recognition · Computer Science 2016-08-09 William Gray Roncal , Colin Lea , Akira Baruah , Gregory D. Hager

Electron microscopic connectomics is an ambitious research direction with the goal of studying comprehensive brain connectivity maps by using high-throughput, nano-scale microscopy. One of the main challenges in connectomics research is…

Computer Vision and Pattern Recognition · Computer Science 2021-04-14 Tran Minh Quan , David G. C. Hildebrand , Won-Ki Jeong

Neural connectomics has begun producing massive amounts of data, necessitating new analysis methods to discover the biological and computational structure. It has long been assumed that discovering neuron types and their relation to…

Neurons and Cognition · Quantitative Biology 2014-07-17 Eric Jonas , Konrad Kording

Connectomics aims to recover a complete set of synaptic connections within a dataset imaged by volume electron microscopy. Many systems have been proposed for locating synapses, and recent research has included a way to identify the…

Computer Vision and Pattern Recognition · Computer Science 2019-11-25 Nicholas Turner , Kisuk Lee , Ran Lu , Jingpeng Wu , Dodam Ih , H. Sebastian Seung

Behavioural differences across organisms, whether healthy or pathological, are closely tied to the structure of their neural circuits. Yet, the fine-scale synaptic changes that give rise to these variations remain poorly understood, in part…

Computer Vision and Pattern Recognition · Computer Science 2025-09-23 Samia Mohinta , Daniel Franco-Barranco , Shi Yan Lee , Albert Cardona

Automatic cell image segmentation methods in connectomics produce merge and split errors, which require correction through proofreading. Previous research has identified the visual search for these errors as the bottleneck in interactive…

Computer Vision and Pattern Recognition · Computer Science 2017-04-05 Daniel Haehn , Verena Kaynig , James Tompkin , Jeff W. Lichtman , Hanspeter Pfister

Volumetric brain reconstructions provide an unprecedented opportunity to gain insights into the complex connectivity patterns of neurons in an increasing number of organisms. Here, we model and quantify the complexity of the resulting…

Neurons and Cognition · Quantitative Biology 2024-05-13 Anastasiya Salova , István A. Kovács

To build the connectomics map of the brain, we developed a new algorithm that can automatically refine the Membrane Detection Probability Maps (MDPM) generated to perform automatic segmentation of electron microscopy (EM) images. To achieve…

Neural and Evolutionary Computing · Computer Science 2015-06-22 Xundong Wu

Mapping the connectivity of neurons in the brain (i.e., connectomics) is a challenging problem due to both the number of connections in even the smallest organisms and the nanometer resolution required to resolve them. Because of this,…

Neurons and Cognition · Quantitative Biology 2014-09-08 Ting Zhao , Stephen M Plaza

Understanding the dynamical behavior of complex systems from their underlying network architectures is a long-standing question in complexity theory. Therefore, many metrics have been devised to extract network features like motifs,…

Neurons and Cognition · Quantitative Biology 2024-09-05 Bryant Avila , Pedro Augusto , David Phillips , Tommaso Gili , Manuel Zimmer , Hernán A. Makse

An open challenge problem at the forefront of modern neuroscience is to obtain a comprehensive mapping of the neural pathways that underlie human brain function; an enhanced understanding of the wiring diagram of the brain promises to lead…

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