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Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique in biomedical research that uses the fluorophore decay rate to provide additional contrast in fluorescence microscopy. However, at present, the calculation, analysis,…

Image and Video Processing · Electrical Eng. & Systems 2021-06-09 Varun Mannam , Yide Zhang , Xiaotong Yuan , Cara Ravasio , Scott S. Howard

In fluid flow imaging, intensity gradients are a good measure of spatial variations in scalar properties, which play an important role in controlling transport processes. However, current flow imaging techniques exhibit system-limited…

Optics · Physics 2025-10-22 Hy Cao , Abhishek Saha , Lisa V. Poulikakos

Single-shot volumetric fluorescence (SVF) imaging offers a significant advantage over traditional imaging methods that require scanning across multiple axial planes as it can capture biological processes with high temporal resolution. The…

Programmable optical devices provide performance enhancement and flexibility to spatial multiplexing systems enabling transmission of tributaries in high-order eigenmodes of spatially-diverse transmission media, like multimode fiber (MMF).…

Optical imaging plays a critical role in advancing our understanding of three dimensional dynamics of biological systems. Coherent imaging (CI) methods exploit spatial phase information, encoded through propagation of coherent signal light…

Optics · Physics 2015-07-24 Jeffrey J. Field , David G. Winters , Randy A. Bartels

MRI (Magnetic Resonance Imaging) is a technique used to analyze and diagnose the problem defined by images like cancer or tumor in a brain. Physicians require good contrast images for better treatment purpose as it contains maximum…

Computer Vision and Pattern Recognition · Computer Science 2020-03-17 Sakshi Patel , Bharath K P , Rajesh Kumar Muthu

Functional Spectral Imaging (FSI) models image formation as the recovery of tissue surrogates such as density and stiffness from spectral perturbations of a self-adjoint elliptic operator. Rather than relying on reflectivity or relaxation…

Medical Physics · Physics 2025-10-21 Cesar Mello Fernando Medina da Cunha

Optical super-oscillation enables far-field super-resolution imaging beyond diffraction limits. However, the existing super-oscillatory lens for the spatial super-resolution imaging system still confronts critical limitations in performance…

The purpose of this study was to present image reconstruction methods for magnetic particle imaging (MPI) with a field-free-line (FFL) encoding scheme and to propose the use of the maximum likelihood-expectation maximization (ML-EM)…

Medical Physics · Physics 2016-06-13 Kenya Murase

With the availability of commercial Light Field (LF) cameras, LF imaging has emerged as an up and coming technology in computational photography. However, the spatial resolution is significantly constrained in commercial microlens based LF…

Computer Vision and Pattern Recognition · Computer Science 2022-07-27 Aupendu Kar , Suresh Nehra , Jayanta Mukhopadhyay , Prabir Kumar Biswas

Although several image super-resolution solutions exist, they still face many challenges. CNN-based algorithms, despite the reduction in computational complexity, still need to improve their accuracy. While Transformer-based algorithms have…

Computer Vision and Pattern Recognition · Computer Science 2024-05-03 Nianzu Qiao , Lamei Di , Changyin Sun

We extend our previously proposed image reconstruction method, which allows confocal microscopes to capture periodically moving objects at frequencies beyond their frame rates, to three-dimensional and two-dimensional wide-field imaging.…

Optics · Physics 2026-03-03 Kanta Adachi , Soyoka Hemmi , Nobutomo Nakamura

We present a superpixel method for full spatial phase and amplitude control of a light beam using a digital micromirror device (DMD) combined with a spatial filter. We combine square regions of nearby micromirrors into superpixels by low…

Optics · Physics 2015-06-19 Sebastianus A. Goorden , Jacopo Bertolotti , Allard P. Mosk

Selective plane illumination microscopy (SPIM), also known as light sheet fluorescence microscopy, provides high specificity through fluorescence labeling. However, it lacks complementary structural information from the surrounding context,…

Optics · Physics 2024-12-19 Rui Ma , Olga Lyraki , Daniel Wehner , Jochen Guck

Image forgery localization (IFL) is a crucial technique for preventing tampered image misuse and protecting social safety. However, due to the rapid development of image tampering technologies, extracting more comprehensive and accurate…

Computer Vision and Pattern Recognition · Computer Science 2024-12-30 Ziqi Sheng , Wei Lu , Xiangyang Luo , Jiantao Zhou , Xiaochun Cao

Intraoperative optical imaging is essential for surgical precision and patient safety, but current systems present anatomical and fluorescence information separately, causing delays and increasing cognitive load. A unified system for…

Medical Physics · Physics 2025-09-22 Kyriakos Pentarakis , George Kakavelakis , Constantinos Petridis , George Themelis

Existing multi-focus image fusion (MFIF) methods often fail to preserve the uncertain transition region and detect small focus areas within large defocused regions accurately. To address this issue, this study proposes a new…

Computer Vision and Pattern Recognition · Computer Science 2024-02-01 Xilai Li , Xiaosong Li , Haishu Tan , Jinyang Li

Nonlinear imaging systems can surpass the limits of linear optics, but to date they have all relied on physical media (e.g. crystals) to work. These materials are all constrained by their physical properties, such as frequency selectivity,…

Optics · Physics 2015-07-31 Jen-Tang Lu , Alexandre S. Goy , Jason W. Fleischer

The Fast Proximal Gradient Method (FPGM) and the Monotone FPGM (MFPGM) for minimization of nonsmooth convex functions are introduced and applied to tomographic image reconstruction. Convergence properties of the sequence of objective…

Optimization and Control · Mathematics 2020-08-25 Elias S. Helou , Marcelo V. W. Zibetti , Gabor T. Herman

Fluorescence lifetime imaging (FLI) is a widely used technique in the biomedical field for measuring the decay times of fluorescent molecules, providing insights into metabolic states, protein interactions, and ligand-receptor bindings.…