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Bacteria tightly regulate and coordinate the various events in their cell cycles to duplicate themselves accurately and to control their cell sizes. Growth of Escherichia coli, in particular, follows a relation known as Schaechter 's growth…

Cell Behavior · Quantitative Biology 2017-01-04 Hai Zheng , Po-Yi Ho , Meiling Jiang , Bin Tang , Weirong Liu , Dengjin Li , Xuefeng Yu , Nancy E. Kleckner , Ariel Amir , Chenli Liu

Uncovering the quantitative laws that govern the growth and division of single cells remains a major challenge. Using a unique combination of technologies that yields unprecedented statistical precision, we find that the sizes of individual…

Microbial growth and division are fundamental processes relevant to many areas of life science. Of particular interest are homeostasis mechanisms, which buffer growth and division from accumulating fluctuations over multiple cycles. These…

Cell Behavior · Quantitative Biology 2022-10-12 Lee Susman , Maryam Kohram , Harsh Vashistha , Jeffrey T. Nechleba , Hanna Salman , Naama Brenner

Various rod-shaped bacteria such as the canonical gram negative Escherichia coli or the well-studied gram positive Bacillus subtilis divide symmetrically after they approximately double their volume. Their size at division is not constant,…

Cell Behavior · Quantitative Biology 2015-06-18 Ariel Amir

Bacterial growth and division generally occur by the process known as binary fission, in which the cells grow polarly until they divide into two daughter cells. Although this process is affected by factors that introduce stochastic…

Soft Condensed Matter · Physics 2022-10-05 Andrés Delgado-Campos , Alejandro Cuetos

A key goal of systems biology is the predictive mathematical description of gene regulatory circuits. Different approaches are used such as deterministic and stochastic models, models that describe cell growth and division explicitly or…

Molecular Networks · Quantitative Biology 2012-10-12 Rahul Marathe , Veronika Bierbaum , David Gomez , Stefan Klumpp

Cell growth in size is a complex process coordinated by intrinsic and environmental signals. In a recent work [Tzur et al., Science, 2009, 325:167-171], size distributions in an exponentially growing population of mammalian cells were used…

Cell Behavior · Quantitative Biology 2015-06-16 Yucheng Hu , Tianqi Zhu

Regulation of cell growth and division is essential to achieve cell-size homeostasis. Recent advances in imaging technologies, such as ``mother machines" for bacteria or yeast, have allowed long-term tracking of cell-size dynamics across…

Quantitative Methods · Quantitative Biology 2024-08-28 Shijie Zhang , Chenyi Fei , Jörn Dunkel

We consider a class of biologically-motivated stochastic processes in which a unicellular organism divides its resources (volume or damaged proteins, in particular) symmetrically or asymmetrically between its progeny. Assuming the final…

Quantitative Methods · Quantitative Biology 2016-07-20 Andrew Marantan , Ariel Amir

How can a microorganism adapt to a variety of environmental conditions despite there exists a limited number of signal transduction machineries? We show that for any growing cells whose gene expression is under stochastic fluctuations,…

Molecular Networks · Quantitative Biology 2015-05-13 Chikara Furusawa , Kunihiko Kaneko

Bacterial physiology is a branch of biology that aims to understand overarching principles of cellular reproduction. Many important issues in bacterial physiology are inherently quantitative, and major contributors to the field have often…

Cell Behavior · Quantitative Biology 2018-03-15 Suckjoon Jun , Fangwei Si , Rami Pugatch , Matthew Scott

The mechanism of bacterial cell size control has been a mystery for decades, which involves the well-coordinated growth and division in the cell cycle. The revolutionary modern techniques of microfluidics and the advanced live imaging…

Statistical Mechanics · Physics 2021-08-30 Liang Luo , Yang Bai , Xiongfei Fu

The rate at which individual bacterial cells grow depends on the concentrations of cellular components such as ribosomes and proteins. These concentrations continuously fluctuate over time and are inherited from mother to daughter cells,…

Populations and Evolution · Quantitative Biology 2024-08-21 Yaïr Hein , Farshid Jafarpour

For various species of biological cells, experimental observations indicate the existence of universal distributions of the cellular size, scaling relations between the cell-size moments and simple rules for the cell-size control. We…

Soft Condensed Matter · Physics 2017-01-12 David A. Kessler , Stanislav Burov

Inside individual cells, expression of genes is inherently stochastic and manifests as cell-to-cell variability or noise in protein copy numbers. Since proteins half-lives can be comparable to the cell-cycle length, randomness in…

Molecular Networks · Quantitative Biology 2015-10-06 Mohammad Soltani , Cesar Augusto Vargas-Garcia , Duarte Antunes , Abhyudai Singh

We study the Stochastic Thermodynamics of cell growth and division using a theoretical framework based on branching processes with resetting. Cell division may be split into two sub-processes: branching, by which a given cell gives birth to…

Statistical Mechanics · Physics 2023-03-01 Arthur Genthon , Reinaldo García-García , David Lacoste

Protein distributions measured under a broad set of conditions in bacteria and yeast were shown to exhibit a common skewed shape, with variances depending quadratically on means. For bacteria these properties were reproduced by temporal…

Biological Physics · Physics 2015-10-28 Naama Brenner , C. M. Newman , Dino Osmanovic , Yitzhak Rabin , Hanna Salman , D. L. Stein

Gene expression is inherently a noisy process which manifests as cell-to-cell variability in time evolution of proteins. Consequently, events that trigger at critical threshold levels of regulatory proteins exhibit stochasticity in their…

Subcellular Processes · Quantitative Biology 2016-09-26 Khem Raj Ghusinga , Abhyudai Singh

How are granular details of stochastic growth and division of individual cells reflected in smooth deterministic growth of population numbers? We provide an integrated, multiscale perspective of microbial growth dynamics by formulating a…

Is it possible to estimate the dependence of a growing and dividing population on a given trait in the case where this trait is not directly accessible by experimental measurements, but making use of measurements of another variable? This…

Statistics Theory · Mathematics 2019-07-12 Marie Doumic , Adélaïde Olivier , Lydia Robert