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The utilization of multiple phosphorylation sites in regulating a biological response is ubiquitous in cell signaling. If each site contributes an additional, equivalent binding site, then one consequence of an increase in the number of…

Subcellular Processes · Quantitative Biology 2009-11-13 Jason W. Locasale

Living things benefit from exquisite molecular sensitivity in many of their key processes, including DNA replication, transcription and translation, chemical sensing, and morphogenesis. At thermodynamic equilibrium, the basic biophysical…

Statistical Mechanics · Physics 2021-12-16 Jeremy A. Owen , Jordan M. Horowitz

Multisite phosphorylation plays an important role in regulating switchlike protein activity and has been used widely in mathematical models. With the development of new experimental techniques and more molecular data, molecular…

Molecular Networks · Quantitative Biology 2020-09-15 Minghan Chen , Mansooreh Ahmadian , Layne Watson , Yang Cao

Multivalent particles have the ability to form multiple bonds to a substrate. Hence, a multivalent interaction can be strong, even if the individual bonds are weak. However, much more interestingly, multivalency greatly increases the…

Soft Condensed Matter · Physics 2017-04-04 Tine Curk , Jure Dobnikar , Daan Frenkel

Multisite phosphorylation plays an important role in intracellular signaling. There has been much recent work aimed at understanding the dynamics of such systems when the phosphorylation/dephosphorylation mechanism is distributive, that is,…

Molecular Networks · Quantitative Biology 2014-10-23 Carsten Conradi , Anne Shiu

Multisite protein phosphorylation plays a pivotal role in regulating cellular signaling and decision-making processes. In this study, we focus on the mathematical underpinnings and informational aspects of sequential, distributive…

Molecular Networks · Quantitative Biology 2025-07-22 Iman Tavassoly , Adel Mehrpooya , Parsa Mirlohi , Zahra Abbaspourasadollah

Post-transductional modifications tune the functions of proteins and regulate the collective dynamics of biochemical networks that determine how cells respond to environmental signals. For example, protein phosphorylation and nitrosylation…

Cell Behavior · Quantitative Biology 2009-11-13 Roberto Chignola , Chiara Dalla Pellegrina , Alessio Del Fabbro , Edoardo Milotti

The sensitivity (i.e. dynamic response) of complex networked systems has not been well understood, making difficult to predict whether new macroscopic dynamic behavior will emerge even if we know exactly how individual nodes behave and how…

Systems and Control · Computer Science 2016-10-18 Marco Tulio Angulo , Gabor Lippner , Yang-Yu Liu , Albert-László Barabási

This study address the computational determination of catalytic reaction rates by moving beyond traditional Transition State Theory (TST), addressing its limitations in complex systems. The Hill relation framework, integrated with Adaptive…

Chemical Physics · Physics 2025-11-25 Thomas Pigeon , Manuel Corral Valero , Pascal Raybaud

Multisite protein modification is a ubiquitous mechanism utilized by cells to control protein functions. We have recently proposed a dynamical description of multisite protein modification which embodies all the essential features of the…

Biological Physics · Physics 2007-05-23 Edoardo Milotti , Alessio Del Fabbro , Chiara Dalla Pellegrina , Roberto Chignola

Ultrasensitive response motifs, which are capable of converting graded stimulus in binary responses, are very well-conserved in signal transduction networks. Although it has been shown that a cascade arrangement of multiple ultrasensitive…

Molecular Networks · Quantitative Biology 2018-09-03 Edgar Altszyler , Alejandra Ventura , Alejandro Colman-Lerner , Ariel Chernomoretz

Biological sensory systems generally operate out of equilibrium, which often leads to their improved performance. Here, we study the sensitivity of ligand concentration for a general receptor model, which is generally in the non-equilibrium…

Biological Physics · Physics 2017-06-27 Takashi Okada

The 70 kDa Heat Shock Proteins Hsp70 have several essential functions in living systems, such as protecting cells against protein aggregation, assisting protein folding, remodeling protein complexes and driving the translocation into…

Biomolecules · Quantitative Biology 2017-07-26 Basile Nguyen , David Hartich , Udo Seifert , Paolo De Los Rios

The clinical need for ultra-sensitive molecular analysis has motivated the development of several endpoint assay technologies capable of single molecule readout. These endpoint assays are now primarily limited by the affinity and…

Biological Physics · Physics 2019-05-22 Derin Sevenler , Jacob Trueb , M. Selim Ünlü

Recent advancements in protein docking site prediction have highlighted the limitations of traditional rigid docking algorithms, like PIPER, which often neglect critical stochastic elements such as solvent-induced fluctuations. These…

Quantitative Methods · Quantitative Biology 2024-12-02 Nanjie Chen , Dongliang Yu , Dmitri Beglov , Mark Kon , Julio Enrique Castrillon-Candas

Multisite phosphorylation networks are encountered in many intracellular processes like signal transduction, cell-cycle control or nuclear signal integration. In this contribution networks describing the phosphorylation and…

Molecular Networks · Quantitative Biology 2013-07-03 Katharina Holstein , Dietrich Flockerzi , Carsten Conradi

The detection of ligand binding sites for proteins is a fundamental step in Structure-Based Drug Design. Despite notable advances in recent years, existing methods, datasets, and evaluation metrics are confronted with several key…

Quantitative Methods · Quantitative Biology 2025-11-12 Jigang Fan , Quanlin Wu , Shengjie Luo , Liwei Wang

Protein functions in cells may be activated or modified by the attachment of several kinds of chemical groups. While protein phosphorylation, i.e. the attachment of a phosphoryl (PO$_3^-$) group, is the most studied form of protein…

Biological Physics · Physics 2015-06-26 Edoardo Milotti , Alessio Del Fabbro , Chiara Dalla Pellegrina , Roberto Chignola

Multivalency is prevalent in various biological systems and applications due to the superselectivity that arises from the cooperativity of multivalent binding. Traditionally, it was thought that weaker individual binding would improve the…

Soft Condensed Matter · Physics 2023-04-13 Xiuyang Xia , Ge Zhang , Massimo Pica Ciamarra , Yang Jiao , Ran Ni

In multi-resolution simulations, different system components are simultaneously modelled at different levels of resolution, these being smoothly coupled together. In the case of enzyme systems, computationally expensive atomistic detail is…

Biological Physics · Physics 2016-11-03 Aoife C. Fogarty , Raffaello Potestio , Kurt Kremer
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