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Hyperspectral stimulated Raman scattering (SRS) microscopy is a label-free technique for biomedical and mineralogical imaging which can suffer from low signal to noise ratios. Here we demonstrate the use of an unsupervised deep learning…

In recent years, scientific Complementary Metal Oxide Semiconductor (sCMOS) devices have been increasingly applied in X-ray detection, thanks to their attributes such as high frame rate, low dark current, high radiation tolerance and low…

Instrumentation and Detectors · Physics 2022-12-14 Yu Hsiao , Zhixing Ling , Chen Zhang , Wenxin Wang , Quan Zhou , Xinyang Wang , Shuang-Nan Zhang , Weimin Yuan

We present Defocusing Microscopy (DM), a bright-field optical microscopy technique able to perform total 3D imaging of transparent objects. By total 3D imaging we mean the determination of the actual shapes of the upper and lower surfaces…

Biological Physics · Physics 2015-06-19 P. M. S. Roma , L. Siman , F. T. Amaral , U. Agero , O. N. Mesquita

Cosmological surveys in the far infrared are known to suffer from confusion. The Bayesian de-blending tool, XID+, currently provides one of the best ways to de-confuse deep Herschel SPIRE images, using a flat flux density prior. This work…

Astrophysics of Galaxies · Physics 2017-07-19 W. J. Pearson , L. Wang , F. F. S. van der Tak , P. D. Hurley , D. Burgarella , S. J. Oliver

This work reports on the development of a new approach to the multiscale computational modelling of the focused electron beam-induced deposition (FEBID), realised using the advanced software packages: MBN Explorer and MBN Studio. Our…

Computational Physics · Physics 2025-06-24 Ilia A. Solov'yov , Alexey Prosvetov , Gennady Sushko , Andrey V. Solov'yov

Nonlinear structured illumination microscopy (nSIM) is an effective approach for super-resolution wide-field fluorescence microscopy with a theoretically unlimited resolution. In nSIM, carefully designed, highly-contrasted illumination…

We report a novel lensless on-chip microscopy platform based on near-field blind ptychographic modulation. In this platform, we place a thin diffuser in between the object and the image sensor for light wave modulation. By blindly scanning…

Image and Video Processing · Electrical Eng. & Systems 2020-02-13 Shaowei Jiang , Jiakai Zhu , Pengming Song , Chengfei Guo , Zichao Bian , Ruihai Wang , Yikun Huang , Shiyao Wang , He Zhang , Guoan Zheng

Structured Illumination Microscopy is a widespread methodology to image live and fixed biological structures smaller than the diffraction limits of conventional optical microscopy. Using recent advances in image up-scaling through deep…

Image and Video Processing · Electrical Eng. & Systems 2021-02-19 Miguel Boland , Edward A. K. Cohen , Seth Flaxman , Mark A. A. Neil

Bird's-eye view (BEV) perception has gained significant attention because it provides a unified representation to fuse multiple view images and enables a wide range of down-stream autonomous driving tasks, such as forecasting and planning.…

Computer Vision and Pattern Recognition · Computer Science 2025-04-04 Shu-Wei Lu , Yi-Hsuan Tsai , Yi-Ting Chen

Serial section electron microscopy (ssEM) is a widely used technique for obtaining volumetric information of biological tissues at nanometer scale. However, accurate 3D reconstructions of identified cellular structures and volumetric…

Image and Video Processing · Electrical Eng. & Systems 2020-02-06 Thanuja D. Ambegoda , Julien N. P. Martel , Jozef Adamcik , Matthew Cook , Richard H. R. Hahnloser

Depth electrodes used in stereoelectroencephalography (sEEG) and deep-brain stimulation (DBS) are essential tools for neural recording and stimulation. Traditional designs have limited spatial resolution, typically 8 to 16 cylindrical…

We demonstrate that a deep neural network can significantly improve optical microscopy, enhancing its spatial resolution over a large field-of-view and depth-of-field. After its training, the only input to this network is an image acquired…

Machine Learning · Computer Science 2017-11-21 Yair Rivenson , Zoltan Gorocs , Harun Gunaydin , Yibo Zhang , Hongda Wang , Aydogan Ozcan

The analysis of neural power spectra plays a crucial role in understanding brain function and dysfunction. While recent efforts have led to the development of methods for decomposing spectral data, challenges remain in performing…

Neurons and Cognition · Quantitative Biology 2024-10-29 Johan Medrano , Nicholas A. Alexander , Robert A. Seymour , Peter Zeidman

We present a novel approach to implement compressive sensing in laser scanning microscopes (LSM), specifically in image scanning microscopy (ISM), using a single-photon avalanche diode (SPAD) array detector. Our method addresses two…

Image and Video Processing · Electrical Eng. & Systems 2023-07-20 Ajay Gunalan , Marco Castello , Simonluca Piazza , Shunlei Li , Alberto Diaspro , Leonardo S. Mattos , Paolo Bianchini

The next generation of imaging surveys, including the Vera C. Rubin Observatory Legacy Survey of Space and Time (LSST), Euclid, and the Nancy Grace Roman Space Telescope, will place unprecedented constraints on cosmology using weak…

Cosmology and Nongalactic Astrophysics · Physics 2025-09-08 Andy Park , Xiangchong Li , Rachel Mandelbaum , Matthew Becker

We introduce a novel framework for metric depth estimation that enhances pretrained diffusion-based monocular depth estimation (DB-MDE) models with stereo vision guidance. While existing DB-MDE methods excel at predicting relative depth,…

Computer Vision and Pattern Recognition · Computer Science 2025-10-22 Tuan Pham , Thanh-Tung Le , Xiaohui Xie , Stephan Mandt

The usage of a GaAsP (gallium arsenide phosphide) photomultiplier for microscopical imaging allows the evaluation of low-light luminescent objects. We designed a setup for collecting a confocal microscopic image signal, which is divided…

Depth estimation is a fundamental task in 3D geometry. While stereo depth estimation can be achieved through triangulation methods, it is not as straightforward for monocular methods, which require the integration of global and local…

Computer Vision and Pattern Recognition · Computer Science 2025-04-15 Jinchang Zhang , Ningning Xu , Hao Zhang , Guoyu Lu

We report on a versatile method to compensate the linear attenuation in a medium, independently of its microscopic origin. The method exploits diffraction-limited Bessel beams and tailored on-axis intensity profiles which are generated…

While transmission electron microscopes (TEM) can achieve a much higher resolution than optical microscopes, they face challenges of damage to samples during the high energy processes involved. Here, we explore using computational ghost…