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Stochasticity is both exploited and controlled by cells. Although the intrinsic stochasticity inherent in biochemistry is relatively well understood, cellular variation, or 'noise', is predominantly generated by interactions of the system…

Molecular Networks · Quantitative Biology 2008-09-18 Vahid Shahrezaei , Julien F Ollivier , Peter S Swain

Gene products (RNAs, proteins) often occur at low molecular counts inside individual cells, and hence are subject to considerable random fluctuations (noise) in copy number over time. Not surprisingly, cells encode diverse regulatory…

Molecular Networks · Quantitative Biology 2015-10-01 Thierry Platini , Mohammad Soltani , Abhyudai Singh

Using an analytically solvable stochastic model, we study the properties of a simple genetic circuit consisting of multiple copies of an self-regulating gene. We analyse how the variation in gene copy number and the mutations changing the…

Molecular Networks · Quantitative Biology 2016-08-17 Jakub Jędrak , Anna Ochab-Marcinek

The bulk of stochastic gene expression models in the literature do not have an explicit description of the age of a cell within a generation and hence they cannot capture events such as cell division and DNA replication. Instead, many…

Subcellular Processes · Quantitative Biology 2020-03-11 Casper H. L. Beentjes , Ruben Perez-Carrasco , Ramon Grima

Exploiting the information provided by the molecular noise of a biological process has proven to be valuable in extracting knowledge about the underlying kinetic parameters and sources of variability from single cell measurements. However,…

Quantitative Methods · Quantitative Biology 2013-08-30 Jakob Ruess , Andreas Milias-Argeitis , John Lygeros

Intrinsic transcriptional noise induced by operator fluctuations is investigated with a simple spin like stochastic model. The effects of transcriptional fluctuations in protein synthesis is probed by coupling transcription and translation…

Other Quantitative Biology · Quantitative Biology 2007-05-23 Guilherme da C. P. Innocentini , Jose E. M. Hornos

Gene expression in a cell entails random reaction events occurring over disparate time scales. Thus, molecular noise that often results in phenotypic and population-dynamic consequences sets a fundamental limit to biochemical signaling.…

Molecular Networks · Quantitative Biology 2009-02-19 C. -M. Ghim , E. Almaas

We present a study investigating the role of mitochondrial variability in generating noise in eukaryotic cells. Noise in cellular physiology plays an important role in many fundamental cellular processes, including transcription,…

Cell Behavior · Quantitative Biology 2015-05-28 Iain G. Johnston , Bernadett Gaal , Ricardo Pires das Neves , Tariq Enver , Francisco J. Iborra , Nick S. Jones

The regulation of cellular function is often controlled at the level of gene transcription. Such genetic regulation usually consists of interacting networks, whereby gene products from a single network can act to control their own…

Biological Physics · Physics 2009-11-06 Jeff Hasty , Joel Pradines , Milos Dolnik , J. J. Collins

We show how one may analytically compute the stationary density of the distribution of molecular constituents in populations of cells in the presence of noise arising from either bursting transcription or translation, or noise in…

Molecular Networks · Quantitative Biology 2015-10-15 Michael C. Mackey , Marta Tyran-Kamińska , Romain Yvinec

A common model of stochastic auto-regulatory gene expression describes promoter switching via cooperative protein binding, effective protein production in the active state and dilution of proteins. Here we consider an extension of this…

Subcellular Processes · Quantitative Biology 2020-04-07 James Holehouse , Abhishek Gupta , Ramon Grima

This paper investigates the stochastic fluctuations of the number of copies of a given protein in a cell. This problem has already been addressed in the past and closed-form expressions of the mean and variance have been obtained for a…

Quantitative Methods · Quantitative Biology 2012-06-05 Vincent Fromion , Emanuele Leoncini , Philippe Robert

The dynamics of cellular chemical reactions are variable due to stochastic noise from intrinsic and extrinsic sources. The intrinsic noise is the intracellular fluctuations of molecular copy numbers caused by the probabilistic encounter of…

Systems and Control · Electrical Eng. & Systems 2023-01-13 Yuta Sakurai , Yutaka Hori

Biomolecular condensates have been shown to play a fundamental role in localizing biochemistry in a cell. RNA is a common constituent of condensates, and can determine their biophysical properties. Functions of biomolecular condensates are…

Subcellular Processes · Quantitative Biology 2023-02-22 Alex Mayer , Grace McLaughlin , Sierra Cole , Amy Gladfelter , Marcus Roper

We present a maximum entropy framework to separate intrinsic and extrinsic contributions to noisy gene expression solely from the profile of expression. We express the experimentally accessible probability distribution of the copy number of…

Subcellular Processes · Quantitative Biology 2015-06-12 Purushottam D. Dixit

Molecular noise in gene regulatory networks has two intrinsic components, one part being due to fluctuations caused by the birth and death of protein or mRNA molecules which are often present in small numbers and the other part arising from…

Molecular Networks · Quantitative Biology 2015-11-17 Davit A. Potoyan , Peter G. Wolynes

Cell systems consist of a huge number of various molecules that display specific patterns of interactions, which have a determining influence on the cell's functioning. In general, such complexity is seen to increase with the complexity of…

Biomolecules · Quantitative Biology 2014-03-14 Marianne Rooman , Jaroslav Albert , Mitia Duerinckx

The intrinsic stochasticity of gene expression can lead to large variations in protein levels across a population of cells. To explain this variability, different sources of mRNA fluctuations ('Poisson' and 'Telegraph' processes) have been…

Biological Physics · Physics 2011-03-02 Vlad Elgart , Tao Jia , Rahul V. Kulkarni

Coupling the control of expression stochasticity (noise) to the ability of expression change (plasticity) can alter gene function and influence adaptation. A number of factors, such as transcription re-initiation, strong chromatin…

Genomics · Quantitative Biology 2012-09-12 Djordje Bajić , Juan F. Poyatos

We introduce a biologically detailed, stochastic model of gene expression describing the multiple rate-limiting steps of transcription, nuclear pre-mRNA processing, nuclear mRNA export, cytoplasmic mRNA degradation and translation of mRNA…

Molecular Networks · Quantitative Biology 2024-01-24 Muhan Ma , Juraj Szavits-Nossan , Abhyudai Singh , Ramon Grima