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Reconstructing neuronal circuits at the level of synapses is a central problem in neuroscience and becoming a focus of the emerging field of connectomics. To date, electron microscopy (EM) is the most proven technique for identifying and…

Quantitative Methods · Quantitative Biology 2014-12-05 Stephen M. Plaza , Toufiq Parag , Gary B. Huang , Donald J. Olbris , Mathew A. Saunders , Patricia K. Rivlin

Neural circuit reconstruction at single synapse resolution is increasingly recognized as crucially important to decipher the function of biological nervous systems. Volume electron microscopy in serial transmission or scanning mode has been…

Computer Vision and Pattern Recognition · Computer Science 2018-05-09 Larissa Heinrich , Jan Funke , Constantin Pape , Juan Nunez-Iglesias , Stephan Saalfeld

Extracting a connectome from an electron microscopy (EM) data set requires identification of neurons and determination of synapses between neurons. As manual extraction of this information is very time-consuming, there has been extensive…

Computer Vision and Pattern Recognition · Computer Science 2016-04-12 Gary B. Huang , Louis K. Scheffer , Stephen M. Plaza

Producing connectomes from electron microscopy (EM) images has historically required a great deal of human proofreading effort. This manual annotation cost is the current bottleneck in scaling EM connectomics, for example, in making larger…

Computer Vision and Pattern Recognition · Computer Science 2025-10-01 Gary B Huang , William M Katz , Stuart Berg , Louis Scheffer

The emerging field of connectomics aims to unlock the mysteries of the brain by understanding the connectivity between neurons. To map this connectivity, we acquire thousands of electron microscopy (EM) images with nanometer-scale…

Quantitative Methods · Quantitative Biology 2016-04-04 Stephen M. Plaza , Stuart E. Berg

Accurately estimating the wiring diagram of a brain, known as a connectome, at an ultrastructure level is an open research problem. Specifically, precisely tracking neural processes is difficult, especially across many image slices. Here,…

Computer Vision and Pattern Recognition · Computer Science 2014-05-09 Ayushi Sinha , William Gray Roncal , Narayanan Kasthuri , Jeff W. Lichtman , Randal Burns , Michael Kazhdan

Reconstructing a synaptic wiring diagram, or connectome, from electron microscopy (EM) images of brain tissue currently requires many hours of manual annotation or proofreading (Kasthuri and Lichtman, 2010; Lichtman and Sanes, 2008; Seung,…

Behavioural differences across organisms, whether healthy or pathological, are closely tied to the structure of their neural circuits. Yet, the fine-scale synaptic changes that give rise to these variations remain poorly understood, in part…

Computer Vision and Pattern Recognition · Computer Science 2025-09-23 Samia Mohinta , Daniel Franco-Barranco , Shi Yan Lee , Albert Cardona

Mapping the connectivity of neurons in the brain (i.e., connectomics) is a challenging problem due to both the number of connections in even the smallest organisms and the nanometer resolution required to resolve them. Because of this,…

Neurons and Cognition · Quantitative Biology 2014-09-08 Ting Zhao , Stephen M Plaza

An open challenge problem at the forefront of modern neuroscience is to obtain a comprehensive mapping of the neural pathways that underlie human brain function; an enhanced understanding of the wiring diagram of the brain promises to lead…

Identifying complex neural circuitry from electron microscopic (EM) images may help unlock the mysteries of the brain. However, identifying this circuitry requires time-consuming, manual tracing (proofreading) due to the size and intricacy…

Quantitative Methods · Quantitative Biology 2014-09-04 Stephen M. Plaza

Despite the progress in deep learning networks, efficient learning at the edge (enabling adaptable, low-complexity machine learning solutions) remains a critical need for defense and commercial applications. We envision a pipeline to…

High-throughput electron microscopy allows recording of lar- ge stacks of neural tissue with sufficient resolution to extract the wiring diagram of the underlying neural network. Current efforts to automate this process focus mainly on the…

Computer Vision and Pattern Recognition · Computer Science 2018-07-17 Julia Buhmann , Renate Krause , Rodrigo Ceballos Lentini , Nils Eckstein , Matthew Cook , Srinivas Turaga , Jan Funke

The current neuron reconstruction pipeline for electron microscopy (EM) data usually includes automatic image segmentation followed by extensive human expert proofreading. In this work, we aim to reduce human workload by predicting…

Computer Vision and Pattern Recognition · Computer Science 2024-01-09 Qihua Chen , Xuejin Chen , Chenxuan Wang , Yixiong Liu , Zhiwei Xiong , Feng Wu

Separating synapses into different classes based on their appearance in EM images has many applications in biology. Examples may include assigning a neurotransmitter to a particular class, or separating synapses whose strength can be…

Computer Vision and Pattern Recognition · Computer Science 2025-10-27 Aarav Shetty , Gary B Huang

Morphology based analysis of cell types has been an area of great interest to the neuroscience community for several decades. Recently, high resolution electron microscopy (EM) datasets of the mouse brain have opened up opportunities for…

The promise of large-scale, high-resolution datasets from Electron Microscopy (EM) and X-ray Microtomography (XRM) lies in their ability to reveal neural structures and synaptic connectivity, which is critical for understanding the brain.…

Synaptic connectivity detection is a critical task for neural reconstruction from Electron Microscopy (EM) data. Most of the existing algorithms for synapse detection do not identify the cleft location and direction of connectivity…

Computer Vision and Pattern Recognition · Computer Science 2018-10-26 Toufiq Parag , Daniel Berger , Lee Kamentsky , Benedikt Staffler , Donglai Wei , Moritz Helmstaedter , Jeff W. Lichtman , Hanspeter Pfister

We present an approach for the joint segmentation and grouping of similar components in anisotropic 3D image data and use it to segment neural tissue in serial sections electron microscopy (EM) images. We first construct a nested set of…

Computer Vision and Pattern Recognition · Computer Science 2011-09-20 Jan Funke , Björn Andres , Fred Hamprecht , Albert Cardona , Matthew Cook

In this work, we propose a learning framework for identifying synapses using a deep and wide multi-scale recursive (DAWMR) network, previously considered in image segmentation applications. We apply this approach on electron microscopy data…

Computer Vision and Pattern Recognition · Computer Science 2014-09-08 Gary B. Huang , Stephen Plaza
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