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Over the last decade, the light microscope has become increasingly useful as a quantitative tool for studying colloidal systems. The ability to obtain particle coordinates in bulk samples from micrographs is particularly appealing. In this…

Soft Condensed Matter · Physics 2007-09-27 Matthew C. Jenkins , Stefan U. Egelhaaf

Confocal microscopy in combination with real-space particle tracking has proven to be a powerful tool in scientific fields such as soft matter physics, materials science and cell biology. However, 3D tracking of anisotropic particles in…

Soft Condensed Matter · Physics 2015-05-20 T. H. Besseling , M. Hermes , A. Kuijk , B. de Nijs , T. -S. Deng , M. Dijkstra , A. Imhof , A. van Blaaderen

In this paper, we propose a new algorithm based on radial symmetry center method to track colloidal particles close to contact, where the optical images of the particles start to overlap in digital video microscopy. This overlapping effect…

Instrumentation and Detectors · Physics 2017-10-26 Harun Yücel , Nazmi Turan Okumuşoğlu

Confocal microscopy of fluorescent labeled particles has been used to study the dynamical and structural properties of colloidal and granular matter in real space. Localization algorithms allow for a fully automatized determination of the…

Soft Condensed Matter · Physics 2011-12-08 Marcel Roth , Michael Franzmann , Maria D'Acunzi , Max Kreiter , Günter K. Auernhammer

The precise tracking of micron sized colloidal particles - held in the vicinity of each other using optical tweezers - is an elegant way to gain information about the particle-particle pair interaction potential. The accuracy of the method,…

Optics · Physics 2017-09-26 Chi Zhang , Georges Brügger , Frank Scheffold

We propose an autofocusing algorithm to obtain, relatively accurately, the 3D position of each particle, particularly its axial location, and particle number of a dense transparent particle solution via its hologram. First, morphological…

Signal Processing · Electrical Eng. & Systems 2025-03-25 Wei-Na Li , Yi Zhou , Jiatai Chen , Hongjie Ou , XiangSheng Xie

Despite significant advances in particle imaging technologies over the past two decades, few advances have been made in particle tracking, i.e. linking individual particle positions across time series data. The state-of-the-art tracking…

Soft Condensed Matter · Physics 2022-01-25 Ella M. King , Zizhao Wang , David A. Weitz , Frans Spaepen , Michael P. Brenner

Quantitative tracking of features from video images is a basic technique employed in many areas of science. Here, we present a method for the tracking of features that partially overlap, in order to be able to track so-called colloidal…

Soft Condensed Matter · Physics 2016-11-24 Casper van der Wel , Daniela J. Kraft

In complex plasmas, the behavior of freely floating micrometer sized particles is studied. The particles can be directly visualized and recorded by digital video cameras. To analyze the dynamics of single particles, reliable algorithms are…

Plasma Physics · Physics 2018-02-14 Daniel P. Mohr , Christina A. Knapek , Peter Huber , Erich Zaehringer

Particle tracking is a key to single-particle-level confocal microscopy observation of colloidal suspensions, emulsions, and granular matter. The conventional tracking method has not been able to provide accurate information on the size of…

Soft Condensed Matter · Physics 2013-01-31 Mathieu Leocmach , Hajime Tanaka

In-line holographic microscopy provides an unparalleled wealth of information about the properties of colloidal dispersions. Analyzing one colloidal particle's hologram with the Lorenz-Mie theory of light scattering yields the particle's…

Soft Condensed Matter · Physics 2020-02-25 Lauren E. Altman , David G. Grier

We present recent advances in the instrumentation and analysis methods for quantitative imaging of concentrated colloidal suspensions under flow. After a brief review of colloidal imaging, we describe various flow geometries for two and and…

Soft Condensed Matter · Physics 2009-09-29 Lucio Isa , Rut Besseling , Eric R. Weeks , Wilson C. K. Poon

In-line digital holography is a valuable tool for sizing, locating and tracking micro- or nano-objects in a volume. When a parametric imaging model is available, Inverse Problems approaches provide a straightforward estimate of the object…

Instrumentation and Detectors · Physics 2023-07-19 Nicolas Verrier , Corinne Fournier , Thierry Fournel

This paper addresses the problem of unsupervised object localization in an image. Unlike previous supervised and weakly supervised algorithms that require bounding box or image level annotations for training classifiers in order to learn…

Computer Vision and Pattern Recognition · Computer Science 2017-06-30 Aditya Vora , Shanmuganathan Raman

This work proposes a novel framework for visual tracking based on the integration of an iterative particle filter, a deep convolutional neural network, and a correlation filter. The iterative particle filter enables the particles to correct…

Computer Vision and Pattern Recognition · Computer Science 2023-01-05 Reza Jalil Mozhdehi , Henry Medeiros

Colloidoscope is a deep learning pipeline employing a 3D residual Unet architecture, designed to enhance the tracking of dense colloidal suspensions through confocal microscopy. This methodology uses a simulated training dataset that…

Three-dimensional particle tracking is an essential tool in studying dynamics under the microscope, namely, fluid dynamics in microfluidic devices, bacteria taxis, cellular trafficking. The 3d position can be determined using 2d imaging…

Computer Vision and Pattern Recognition · Computer Science 2015-09-08 Eldad Afik

The increasing use of microfluidics in industrial, biomedical, and clinical applications requires a more and more precise control of the microfluidic flows and suspended particles or cells. This leads to higher demands in three-dimensional…

Fluid Dynamics · Physics 2020-11-10 Massimiliano Rossi , Rune Barnkob

Iterative projection algorithms are successfully being used as a substitute of lenses to recombine, numerically rather than optically, light scattered by illuminated objects. Images obtained computationally allow aberration-free…

Optics · Physics 2007-05-23 S. Marchesini

Despite its potential for label-free particle diagnostics, holographic microscopy is limited by specialized processing methods that struggle to generalize across diverse settings. We introduce a deep learning architecture leveraging human…

Optics · Physics 2024-11-26 Shyam Kumar , Jiarong Hong
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