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In genetic circuits, when the mRNA lifetime is short compared to the cell cycle, proteins are produced in geometrically-distributed bursts, which greatly affects the cellular switching dynamics between different metastable phenotypic…

Statistical Mechanics · Physics 2016-05-25 Shay Be'er , Metar Heller-Algazi , Michael Assaf

We consider a stochastic model of gene expression in which transcription depends on a multistate promoter, including the famous two-state model and refractory promoters as special cases, and focus on deriving the exact stationary…

Probability · Mathematics 2019-08-05 Ulysse Herbach

Many biological systems can be described by finite Markov models. A general method for simplifying master equations is presented that is based on merging adjacent states. The approach preserves the steady-state probability distribution and…

Biological Physics · Physics 2021-03-01 David Seiferth , Peter Sollich , Stefan Klumpp

Coarse-grained models have played an important role in the study of the behavior of DNA at length scales beyond a few hundred base pairs. Traditionally, these models have relied on structurally featureless and sequence-independent…

Soft Condensed Matter · Physics 2024-09-10 Enrico Skoruppa , Helmut Schiessel

The telegraph model is the standard model of stochastic gene expression, which can be solved exactly to obtain the distribution of mature RNA numbers per cell. A modification of this model also leads to an analytical distribution of the…

Subcellular Processes · Quantitative Biology 2023-08-17 Juraj Szavits-Nossan , Ramon Grima

In biophysics, the search for analytical solutions of stochastic models of cellular processes is often a challenging task. In recent work on models of gene expression, it was shown that a mapping based on partitioning of Poisson arrivals…

Biological Physics · Physics 2018-05-09 Hugo Tschirhart , Thierry Platini

We extend a recently developed exact schema based, or coarse grained, formulation of genetic dynamics \cite{stewael,stewael1,stewael2} and its associated exact Schema theorem to an arbitrary selection scheme and a general crossover…

Adaptation and Self-Organizing Systems · Physics 2007-05-23 C. R. Stephens

A generalized understanding of protein dynamics is an unsolved scientific problem, the solution of which is critical to the interpretation of the structure-function relationships that govern essential biological processes. Here, we approach…

It is well established that gene expression can be modeled as a Markovian stochastic process and hence proper observables might be subjected to large fluctuations and rare events. Since dynamics is often more than statics, one can work with…

Biological Physics · Physics 2019-09-11 Pegah Torkaman , Farhad H. Jafarpour

The techniques of data-driven backmapping from coarse-grained (CG) to fine-grained (FG) representation often struggle with accuracy, unstable training, and physical realism, especially when applied to complex systems such as proteins. In…

Machine Learning · Computer Science 2025-05-26 Georgios Kementzidis , Erin Wong , John Nicholson , Ruichen Xu , Yuefan Deng

We study a stochastic model of biosynthesis of proteins in generic bacterial operons. The stochasticity arises from two different processes, namely from `bursting' production of either mRNA and/or protein (in the transcription/translation…

Molecular Networks · Quantitative Biology 2018-11-20 Mateusz Falfus , Michael C. Mackey , Marta Tyran-Kaminska

We propose a data-driven, coarse-graining formulation in the context of equilibrium statistical mechanics. In contrast to existing techniques which are based on a fine-to-coarse map, we adopt the opposite strategy by prescribing a…

Machine Learning · Statistics 2017-02-01 Markus Schöberl , Nicholas Zabaras , Phaedon-Stelios Koutsourelakis

Stochastic simulation can make the molecular processes of cellular control more vivid than the traditional differential-equation approach by generating typical system histories instead of just statistical measures such as the mean and…

Subcellular Processes · Quantitative Biology 2018-09-18 Kevin Y. Chen , Daniel M. Zuckerman , Philip C. Nelson

Mathematical modelling of biological population dynamics often involves proposing high fidelity discrete agent-based models that capture stochasticity and individual-level processes. These models are often considered in conjunction with an…

Dynamical Systems · Mathematics 2023-12-20 Daniel J. VandenHeuvel , Pascal R. Buenzli , Matthew J. Simpson

Transcriptional pulsing has been observed in both prokaryotes and eukaryotes and plays a crucial role in cell to cell variability of protein and mRNA numbers. The issue is how the time constants associated with episodes of transcriptional…

Quantitative Methods · Quantitative Biology 2009-09-29 Srividya Iyer-Biswas , F. Hayot , C. Jayaprakash

We propose a probabilistic model for interpreting gene expression levels that are observed through single-cell RNA sequencing. In the model, each cell has a low-dimensional latent representation. Additional latent variables account for…

Machine Learning · Computer Science 2017-10-18 Romain Lopez , Jeffrey Regier , Michael Cole , Michael Jordan , Nir Yosef

From the response to external stimuli to cell division and death, the dynamics of living cells is based on the expression of specific genes at specific times. The decision when to express a gene is implemented by the binding and unbinding…

Molecular Networks · Quantitative Biology 2009-11-13 Johannes Berg

Gene regulation is an important fundamental biological process. The regulation of gene expression is managed through a variety of methods including epigenetic processes (e.g., DNA methylation). Understanding the role of epigenetic changes…

Molecular Networks · Quantitative Biology 2021-06-01 James Brunner , Jacob Kim , Timothy Downing , Eric Mjolsness , Kord M. Kober

Stochastic models of gene expression are typically formulated using the chemical master equation, which can be solved exactly or approximately using a repertoire of analytical methods. Here, we provide a tutorial review of an alternative…

Subcellular Processes · Quantitative Biology 2024-04-19 Juraj Szavits-Nossan , Ramon Grima

Single-cell gene expression measurements encode variability spanning molecular noise, cell-to-cell heterogeneity, and technical artifacts. Mechanistic stochastic models provide powerful approaches to disentangle these sources, yet inferring…

Quantitative Methods · Quantitative Biology 2025-09-19 Christopher E. Miles