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We introduce an image cytometer (I-CYT) for the analysis of phytoplankton in fresh and marine water environments. A linear quantification of cell numbers was observed covering several orders of magnitude using cultures of Tetraselmis and…
We report a field-portable and cost-effective imaging flow cytometer that uses deep learning to accurately detect Giardia lamblia cysts in water samples at a volumetric throughput of 100 mL/h. This flow cytometer uses lensfree color…
Micro and nanoscale particles are crucial in various fields, from biomedical imaging to environmental processes. While conventional spectroscopy and microscopy methods for characterizing these particles often involve bulky equipment and…
A Rapid and cost-effective method for detecting bacterial cells on surfaces is critical to protect public health from various aspects, including food safety, clinical hygiene, and pharmacy quality. Herein, we first established an optical…
This paper reports on the development of a novel CMOS device employing lead-sensing bacteria to assay lead in drinking water. The objective of the PlomBOX project is to develop a low-cost sensor ({\pounds}10) which can expedite access to…
We report the implementation of a fully on-chip, lensless microscopy technique termed optofluidic ptychography. This imaging modality complements the miniaturization provided by microfluidics and allows the integration of ptychographic…
The author discusses the laser interference method to measure the size of small bio-particles: extracellular vesicles (EVs), exosomes and viruses of nanometer scale in flow cytometry. By introducing a new laser configuration in place of…
A low cost fluorescence-based optical system is developed for detecting the presence of certain microorganisms and molecules within a diluted sample. A specifically designed device setup compatible with conventional 96 well plates is chosen…
Increasing demand for understanding the vast heterogeneity of cellular phenotypes has driven the development of imaging flow cytometry (IFC), that combines features of flow cytometry with fluorescence and bright field microscopy. IFC…
Microplastics are increasingly recognized as a global environmental health threat, yet their detection and characterization remain constrained by the cost, form factor, and throughput of existing analytical tools. Portable…
Flow cytometry is a widespread and powerful technique, whose resolution is determined by its capacity to accurately distinguish fluorescently positive populations from negative ones. However, most informative results are discarded while…
In this article we present a new diagnostic approach utilizing flow-cytometry to study compounds of nanoparticle samples in solution by analysis of their scattering patterns retrieved from the cytometric measurements. As a specific…
Portable low-cost sensors and sensing systems for the identification and quantitative measurement of bacteria in field water are critical in preventing drinking water from being contaminated by bacteria. In this article, we reported the…
Rapid identification of bacterial species is crucial in medicine and food hygiene. In order to achieve rapid and label-free identification of bacterial species at the single bacterium level, we propose and experimentally demonstrate an…
Rapid identification of microparticles in liquid is an important problem in environmental and biomedical applications such as for microplastic detection in water sources and physiological fluids. Existing spectro-scopic techniques are…
The size- and fluorescence-based sorting of micro- and nano-scale particles suspended in fluid presents a significant and important challenge for both sample analysis and for manufacturing of nanoparticle-based products. Here we demonstrate…
Early detection and identification of pathogenic bacteria such as Escherichia coli (E. coli) is an essential task for public health. The conventional culture-based methods for bacterial colony detection usually take >24 hours to get the…
The ocean is filled with phytoplankton that contribute as much photosynthesis as all land plants combined, making them vital to the carbon cycle and climate system. Recent advances in flow cytometry allow oceanographers to measure the…
We present a fast, high-throughput method for characterizing the motility of microorganisms in 3D based on standard imaging microscopy. Instead of tracking individual cells, we analyse the spatio-temporal fluctuations of the intensity in…
This paper presents SAMSON, a Spectral Absorption-fluorescence Microscopy System for ON-site-imaging of algae within a water sample. Designed to be portable and low-cost for on-site use, the optical sub-system of SAMSON consists of a…