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The Single Particles, Clusters and Biomolecules (SPB) instrument at the European XFEL is located behind the SASE1 undulator, and aims to support imaging and structure determination of biological specimen between about 0.1 micrometer and 1…

Live cell culture is crucial in biomedical studies for analyzing cell properties and dynamics in vitro. This study focuses on segmenting unstained live cells imaged with bright-field microscopy. While many segmentation approaches exist for…

Quantitative Methods · Quantitative Biology 2025-08-26 Surajit Das , Gourav Roy , Pavel Zun

Protein-based therapeutics play a pivotal role in modern medicine targeting various diseases. Despite their therapeutic importance, these products can aggregate and form subvisible particles (SvPs), which can compromise their efficacy and…

Computer Vision and Pattern Recognition · Computer Science 2025-01-28 Michaela Cohrs , Shiwoo Koak , Yejin Lee , Yu Jin Sung , Wesley De Neve , Hristo L. Svilenov , Utku Ozbulak

Magnetic resonance imaging (MRI) is a valuable clinical tool for displaying anatomical structures and aiding in accurate diagnosis. Medical image super-resolution (SR) reconstruction using deep learning techniques can enhance lesion…

Computer Vision and Pattern Recognition · Computer Science 2023-05-30 Xin Hua , Zhijiang Du , Hongjian Yu , Jixin Maa

Single-particle electron cryomicroscopy (cryo-EM) is an increasingly popular technique for elucidating the three-dimensional structure of proteins and other biologically significant complexes at near-atomic resolution. It is an imaging…

Computational Physics · Physics 2020-04-01 Amit Singer , Fred J. Sigworth

Live cell microscopy sequences exhibit complex spatial structures and complicated temporal behaviour, making their analysis a challenging task. Considering cell segmentation problem, which plays a significant role in the analysis, the…

Computer Vision and Pattern Recognition · Computer Science 2019-01-08 Assaf Arbelle , Tammy Riklin Raviv

Multiplex Imaging (MI) enables the simultaneous visualization of multiple biological markers in separate imaging channels at subcellular resolution, providing valuable insights into cell-type heterogeneity and spatial organization. However,…

Image and Video Processing · Electrical Eng. & Systems 2024-11-07 Simon Gutwein , Daria Lazic , Thomas Walter , Sabine Taschner-Mandl , Roxane Licandro

The capability of accurate prediction of protein functions and properties is essential in the biotechnology industry, e.g. drug development and artificial protein synthesis, etc. The main challenges of protein function prediction are the…

Quantitative Methods · Quantitative Biology 2021-12-02 Wei-Cheng Tseng , Po-Han Chi , Jia-Hua Wu , Min Sun

Staphylococcus aureus is an important pathogen, giving rise to antimicrobial resistance in cell strains such as Methicillin Resistant S. aureus (MRSA). Here we report an image analysis framework for automated detection and image…

Biological Physics · Physics 2016-10-21 Adam J. M. Wollman , Helen Miller , Simon Foster , Mark C. Leake

In this contribution, a design of a synthetic calibration genetic circuit to characterize the relative strength of different sensing promoters is proposed and its specifications and performance are analyzed via an effective mathematical…

Molecular Networks · Quantitative Biology 2026-02-06 D. Gamermann , A. Montagud , P. Aparicio , E. Navarro , J. Triana , F. R. Villatoro , J. F. Urchueguía , P. Fernández de Córdoba

Several processes in the cell, such as gene regulation, start when key proteins recognise and bind to short DNA sequences. However, as these sequences can be hundreds of million times shorter than the genome, they are hard to find by simple…

Subcellular Processes · Quantitative Biology 2021-01-27 Markus Nyberg , Tobias Ambjörnsson , Per Stenberg , and Ludvig Lizana

We present the use of single-cell entropy (scEntropy) to measure the order of the cellular transcriptome profile from single-cell RNA-seq data, which leads to a method of unsupervised cell type classification through scEntropy followed by…

Quantitative Methods · Quantitative Biology 2020-02-18 Jingxin Liu , You Song , Jinzhi Lei

We present an automated approach for identifying and annotating motifs and domains in protein sequences, using pretrained Protein Language Models (PLMs) and Concept Activation Vectors (CAVs), adapted from interpretability research in…

Quantitative Methods · Quantitative Biology 2025-11-27 Ahmad Shamail , Claire D. McWhite

Formation, maintenance and physiology of high-density protein-enriched organized nanodomains, first observed in electron microscopy images, remains challenging to investigate due to their small sizes. However, these regions regulate…

Quantitative Methods · Quantitative Biology 2023-12-29 Pierre Parutto , Jennifer Heck , Martin Heine , David Holcman

The ability to quickly and accurately identify microbial species in a sample, known as metagenomic profiling, is critical across various fields, from healthcare to environmental science. This paper introduces a novel method to profile…

Genomics · Quantitative Biology 2025-04-10 Riselda Kodra , Hadjer Benmeziane , Irem Boybat , William Andrew Simon

Cryo-electron microscopy (cryo-EM) remains pivotal in structural biology, yet the task of protein particle picking, integral for 3D protein structure construction, is laden with manual inefficiencies. While recent AI tools such as Topaz and…

Computer Vision and Pattern Recognition · Computer Science 2023-11-29 Fei He , Zhiyuan Yang , Mingyue Gao , Biplab Poudel , Newgin Sam Ebin Sam Dhas , Rajan Gyawali , Ashwin Dhakal , Jianlin Cheng , Dong Xu

Single molecule localization microscopy is a recently developed superresolution imaging technique to visualize structural properties of single cells. The basic principle consists in chemically attaching fluorescent dyes to the molecules,…

Understanding the dynamic nature of protein structures is essential for comprehending their biological functions. While significant progress has been made in predicting static folded structures, modeling protein motions on microsecond to…

The spatio-temporal organization of proteins within the cell membrane can affect numerous biological functions, including cell signaling, communication, and transportation. Deviations from normal spatial arrangements have been observed in…

Biomolecules · Quantitative Biology 2023-10-25 Yasaman Moradi , Jerry SH Lee , Andrea M. Armani

{\it De novo} protein sequencing is essential for understanding cellular processes that govern the function of living organisms and all post-translational events and other sequence modifications that occur after a protein has been…

Biological Physics · Physics 2015-09-17 P. Boynton , M. Di Ventra