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The prospect of neural reconstruction from Electron Microscopy (EM) images has been elucidated by the automatic segmentation algorithms. Although segmentation algorithms eliminate the necessity of tracing the neurons by hand, significant…

Computer Vision and Pattern Recognition · Computer Science 2015-09-29 Toufiq Parag

Biological membranes are one of the most basic structures and regions of interest in cell biology. In the study of membranes, segment extraction is a well-known and difficult problem because of impeding noise, directional and thickness…

Computer Vision and Pattern Recognition · Computer Science 2018-10-24 Joris Roels , Jonas De Vylder , Jan Aelterman , Yvan Saeys , Wilfried Philips

Deep neural networks have achieved satisfactory performance in piles of medical image analysis tasks. However the training of deep neural network requires a large amount of samples with high-quality annotations. In medical image…

Computer Vision and Pattern Recognition · Computer Science 2020-07-08 Jixin Wang , Sanping Zhou , Chaowei Fang , Le Wang , Jinjun Wang

Automated sample preparation and electron microscopy enables acquisition of very large image data sets. These technical advances are of special importance to the field of neuroanatomy, as 3D reconstructions of neuronal processes at the nm…

Neural circuits can be reconstructed from brain images acquired by serial section electron microscopy. Image analysis has been performed by manual labor for half a century, and efforts at automation date back almost as far. Convolutional…

Computer Vision and Pattern Recognition · Computer Science 2019-05-01 Kisuk Lee , Nicholas Turner , Thomas Macrina , Jingpeng Wu , Ran Lu , H. Sebastian Seung

Accurate segmentation of 3-D cell nuclei in microscopy images is essential for the study of nuclear organization, gene expression, and cell morphodynamics. Current image segmentation methods are challenged by the complexity and variability…

Image and Video Processing · Electrical Eng. & Systems 2022-03-18 Sundaresh Ram , Jeffrey J. Rodriguez

Modern deep neural networks achieved remarkable progress in medical image segmentation tasks. However, it has recently been observed that they tend to produce overconfident estimates, even in situations of high uncertainty, leading to…

Computer Vision and Pattern Recognition · Computer Science 2023-06-05 Agostina Larrazabal , Cesar Martinez , Jose Dolz , Enzo Ferrante

Purpose: Conventional automated segmentation of the head anatomy in MRI distinguishes different brain and non-brain tissues based on image intensities and prior tissue probability maps (TPM). This works well for normal head anatomies, but…

Image and Video Processing · Electrical Eng. & Systems 2021-05-20 Lukas Hirsch , Yu Huang , Lucas C Parra

In this paper we present a methodology that uses convolutional neural networks (CNNs) for segmentation by iteratively growing predicted mask regions in each coordinate direction. The CNN is used to predict class probability scores in a…

Image and Video Processing · Electrical Eng. & Systems 2020-09-25 John Lagergren , Erica Rutter , Kevin Flores

Automatic segmentation in MR brain images is important for quantitative analysis in large-scale studies with images acquired at all ages. This paper presents a method for the automatic segmentation of MR brain images into a number of tissue…

Computer Vision and Pattern Recognition · Computer Science 2017-04-12 Pim Moeskops , Max A. Viergever , Adriënne M. Mendrik , Linda S. de Vries , Manon J. N. L. Benders , Ivana Išgum

In the field of connectomics, neuroscientists seek to identify cortical connectivity comprehensively. Neuronal boundary detection from the Electron Microscopy (EM) images is often done to assist the automatic reconstruction of neuronal…

Computer Vision and Pattern Recognition · Computer Science 2017-08-01 Wei Shen , Bin Wang , Yuan Jiang , Yan Wang , Alan Yuille

In the deep metric learning approach to image segmentation, a convolutional net densely generates feature vectors at the pixels of an image. Pairs of feature vectors are trained to be similar or different, depending on whether the…

Computer Vision and Pattern Recognition · Computer Science 2019-02-04 Kyle Luther , H. Sebastian Seung

Object segmentation and structure localization are important steps in automated image analysis pipelines for microscopy images. We present a convolution neural network (CNN) based deep learning architecture for segmentation of objects in…

Computer Vision and Pattern Recognition · Computer Science 2019-01-24 Shan E Ahmed Raza , Linda Cheung , Muhammad Shaban , Simon Graham , David Epstein , Stella Pelengaris , Michael Khan , Nasir M. Rajpoot

We present a method for segmenting neuron membranes in 2D electron microscopy imagery. This segmentation task has been a bottleneck to reconstruction efforts of the brain's synaptic circuits. One common problem is the misclassification of…

Computer Vision and Pattern Recognition · Computer Science 2020-02-05 Thanuja D. Ambegoda , Matthew Cook

The most established method of reconstructing neural circuits from animals involves slicing tissue very thin, then taking mosaics of electron microscope (EM) images. To trace neurons across different images and through different sections,…

Quantitative Methods · Quantitative Biology 2013-04-23 Louis K. Scheffer , Bill Karsh , Shiv Vitaladevun

The emerging field of connectomics aims to unlock the mysteries of the brain by understanding the connectivity between neurons. To map this connectivity, we acquire thousands of electron microscopy (EM) images with nanometer-scale…

Quantitative Methods · Quantitative Biology 2016-04-04 Stephen M. Plaza , Stuart E. Berg

The recent emergence of deep learning has led to a great deal of work on designing supervised deep semantic segmentation algorithms. As in many tasks sufficient pixel-level labels are very difficult to obtain, we propose a method which…

Computer Vision and Pattern Recognition · Computer Science 2024-04-19 Matthias Schwab , Agnes Mayr , Markus Haltmeier

We aim to improve segmentation through the use of machine learning tools during region agglomeration. We propose an active learning approach for performing hierarchical agglomerative segmentation from superpixels. Our method combines…

Computer Vision and Pattern Recognition · Computer Science 2015-03-13 Juan Nunez-Iglesias , Ryan Kennedy , Toufiq Parag , Jianbo Shi , Dmitri B. Chklovskii

Automated and semi-automated techniques in biomedical electron microscopy (EM) enable the acquisition of large datasets at a high rate. Segmentation methods are therefore essential to analyze and interpret these large volumes of data, which…

Computer Vision and Pattern Recognition · Computer Science 2023-08-08 Anusha Aswath , Ahmad Alsahaf , Ben N. G. Giepmans , George Azzopardi

Many functional and structural neuroimaging studies call for accurate morphometric segmentation of different brain structures starting from image intensity values of MRI scans. Current automatic (multi-) atlas-based segmentation strategies…

Image and Video Processing · Electrical Eng. & Systems 2019-09-27 Dennis Bontempi , Sergio Benini , Alberto Signoroni , Michele Svanera , Lars Muckli
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