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DNA is subject to large deformations in a wide range of biological processes. Two key examples illustrate how such deformations influence the readout of the genetic information: the sequestering of eukaryotic genes by nucleosomes, and DNA…

Biomolecules · Quantitative Biology 2015-11-10 Stephanie Johnson , Martin Lindén , Rob Phillips

Much of the complexity observed in gene regulation originates from cooperative protein-DNA binding. While studies of the target search of proteins for their specific binding sites on the DNA have revealed design principles for the…

Biomolecules · Quantitative Biology 2015-05-30 Nico Geisel , Ulrich Gerland

Following recent discoveries of colocalization of downstream-regulating genes in living cells, the impact of the spatial distance between such genes on the kinetics of gene product formation is increasingly recognized. We here show from…

Subcellular Processes · Quantitative Biology 2015-06-15 Otto Pulkkinen , Ralf Metzler

Phenotypes of individuals in a population of organisms are not fixed. Phenotypic fluctuations, which describe temporal variation of the phenotype of an individual or individual-to-individual variation across a population, are present in…

Populations and Evolution · Quantitative Biology 2018-08-15 Hong-Yan Shih , Harry Mickalide , David T. Fraebel , Nigel Goldenfeld , Seppe Kuehn

Genomic expression depends critically both on the ability of regulatory proteins to locate specific target sites on a DNA within seconds and on the formation of long lived (many minutes) complexes between these proteins and the DNA.…

Soft Condensed Matter · Physics 2011-08-23 O. Bénichou , Y. Kafri , M. Sheinman , R. Voituriez

We consider a stochastic model of transcription factor (TF)-regulated gene expression. The model describes two genes: Gene A and Gene B which synthesize the TFs and the target gene proteins respectively. We show through analytic…

Quantitative Methods · Quantitative Biology 2009-11-13 Rajesh Karmakar , Indrani Bose

Motivation: Recent studies of genomic-scale regulatory networks suggested that a feed-forward loop (FFL) circuitry is a key component of many such networks. This led to a study of the functional properties of different FFL types, where the…

Molecular Networks · Quantitative Biology 2008-02-26 Yonatan Bilu

The two most fundamental processes describing change in biology, development and evolu-tion, occur over drastically different timescales, difficult to reconcile within a unified framework. Development involves temporal sequences of cell…

Biological Physics · Physics 2020-09-08 Enrico Borriello , Sara I. Walker , Manfred D. Laubichler

Living cells must control the reading out or "expression" of information encoded in their genomes, and this regulation often is mediated by transcription factors--proteins that bind to DNA and either enhance or repress the expression of…

Molecular Networks · Quantitative Biology 2013-08-01 Gašper Tkačik , Aleksandra M Walczak , William Bialek

Cells may control fluctuations in protein levels by means of negative autoregulation, where transcription factors bind DNA sites to repress their own production. Theoretical studies have assumed a single binding site for the repressor,…

Molecular Networks · Quantitative Biology 2017-05-24 Iván M. Lengyel , Luis G. Morelli

It is tempting to believe that we now own the genome. The ability to read and re-write it at will has ushered in a stunning period in the history of science. Nonetheless, there is an Achilles heel exposed by all of the genomic data that has…

The discovery of motifs underlying gene expression is a challenging one. Some of these motifs are known transcription factors, but sequence inspection often provides valuable clues, even discovery of novel motifs with uncharacterized…

Genomics · Quantitative Biology 2007-05-23 Arvind Rao , Alfred O. Hero , David J. States , James Douglas Engel

The interaction between proteins and DNA is a key driving force in a significant number of biological processes such as transcriptional regulation, repair, recombination, splicing, and DNA modification. The identification of DNA-binding…

Quantitative Methods · Quantitative Biology 2017-05-10 Hamid Reza Hassanzadeh , Pushkar Kolhe , Charles L. Isbell , May D. Wang

Transcription commonly occurs in bursts, with alternating productive (ON) and quiescent (OFF) periods, governing mRNA production rates. Yet, how transcription is regulated through bursting dynamics remains unresolved. Here, we conduct…

Molecular Networks · Quantitative Biology 2025-11-11 Po-Ta Chen , Michal Levo , Benjamin Zoller , Thomas Gregor

In computational molecular biology, gene regulatory binding sites prediction in whole genome remains a challenge for the researchers. Now a days, the genome wide regulatory binding site prediction tools required either direct pattern…

Genomics · Quantitative Biology 2010-02-06 Chandra Prakash Singh , Feroz Khan , Sanjay Kumar Singh , Durg Singh Chauhan

Expression of cellular genes is regulated by binding of transcription factors to their promoter, either activating or inhibiting transcription of a gene. Particularly interesting is the case when the expressed protein regulates its own…

Molecular Networks · Quantitative Biology 2016-11-28 Martin Jansen

To survive environmental conditions, cells transcribe their response activities into encoded mRNA sequences in order to produce certain amounts of protein concentrations. The external conditions are mapped into the cell through the…

Biological Physics · Physics 2017-10-26 Andrés F. López-Lopera , Mauricio A. Álvarez

Despite significant progress in structural and functional characterization of human genome, understanding of mechanisms underlying the genetic basis of human phenotypic uniqueness remains limited. We report that non-randomly distributed…

Genomics · Quantitative Biology 2025-12-30 Gennadi Glinsky

Understanding the patterns and causes of phenotypic divergence is a central goal in evolutionary biology. Much work has shown that mRNA abundance is highly variable between closely related species. However, the extent and mechanisms of…

Genomics · Quantitative Biology 2013-12-09 Joel McManus , Gemma May , Pieter Spealman , Alan Shteyman

A recurring motif in gene regulatory networks is transcription factors (TFs) that regulate each other, and then bind to overlapping sites on DNA, where they interact and synergistically control transcription of a target gene. Here, we…

Molecular Networks · Quantitative Biology 2013-03-07 Sahand Hormoz