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The imaging performance of an optical microscope can be degraded by sample-induced aberrations. A general strategy to undo the effect of these aberrations is to apply wavefront correction with a deformable mirror (DM). In most cases, the DM…

Optics · Physics 2015-06-23 Jerome Mertz , Hari Paudel , Thomas G. Bifano

In astronomy or biological imaging, refractive index inhomogeneities of e.g. atmosphere or tissues induce optical aberrations which degrade the desired information hidden behind the medium. A standard approach consists in measuring these…

Optics · Physics 2023-06-01 Tengfei Wu , Marc Guillon , Gilles Tessier , Pascal Berto

We describe an adaptive optics technique for two-photon microscopy in which the deformable mirror used for aberration compensation is positioned in a plane conjugate to the plane of the aberration. We demonstrate in a proof-of-principle…

Optics · Physics 2023-07-19 Hari P. Paudel , John Taranto , Jerome Mertz , Thomas Bifano

Adaptive optics in combination with multi-photon techniques is a powerful approach to image deep into a specimen. Remarkably, virtually all adaptive optics schemes today rely on wavefront modulators which are reflective, diffractive, or…

We present a promising approach to the extremely fast sensing and correction of small wavefront errors in adaptive optics systems. As our algorithm's computational complexity is roughly proportional to the number of actuators, it is…

Instrumentation and Methods for Astrophysics · Physics 2016-11-26 Christoph U. Keller , Visa Korkiakoski , Niek Doelman , Rufus Fraanje , Raluca Andrei , Michel Verhaegen

The capability of the adaptive optics to correct for the segmentation error is analyzed in terms of the residual wavefront RMS and the power spectral density of the phase. The analytical model and the end-to-end simulation give…

Astrophysics · Physics 2009-11-11 Natalia Yaitskova , Christophe Verinaud

Aberrations limit scanning fluorescence microscopy when imaging in scattering materials such as biological tissue. Model-based approaches for adaptive optics take advantage of a computational model of the optical setup. Such models can be…

Optics · Physics 2021-07-07 Ivan Vishniakou , Johannes D. Seelig

Adaptive optics (AO) has revolutionized imaging in {fields} from astronomy to microscopy by correcting optical aberrations. In label-free microscopes, however, conventional AO faces limitations due to the absence of guidestar and the need…

Aberrations limit optical systems in many situations, for example when imaging in biological tissue. Machine learning offers novel ways to improve imaging under such conditions by learning inverse models of aberrations. Learning requires…

Optics · Physics 2021-04-30 Ivan Vishniakou , Johannes D. Seelig

Optical imaging through scattering media is a fundamental challenge in many applications. Recently, substantial breakthroughs such as imaging through biological tissues and looking around corners have been obtained by the use of…

Optics · Physics 2020-07-09 Tomer Yeminy , Ori Katz

This work introduces the first closed-loop adaptive optics (AO) system capable of optically correcting aberrations in real-time without a guidestar or a wavefront sensor. Nearly 40 years ago, Cederquist et al. demonstrated that asymmetric…

Image and Video Processing · Electrical Eng. & Systems 2026-05-14 Weiyun Jiang , Haiyun Guo , Christopher A. Metzler , Ashok Veeraraghavan

Inhomogeneities in the refractive index of a biological sample can introduce phase aberrationsin microscopy systems, severely impairing the quality of images. Adaptive optics can be employed to correct for phase aberrations and improve…

We present an analytical model of a single natural guide star astronomical adaptive optics system, in closed loop mode. The model is used to simulate the long exposure system point spread function, using the spatial frequency (or Fourier)…

Instrumentation and Methods for Astrophysics · Physics 2015-05-19 Laurent Jolissaint

Adaptive optics can be used to mitigate the effects of atmospheric turbulence on imaging systems, but the correction is only partial, and deconvolution is often required to improve the resolution. This results in entire optical/digital…

Instrumentation and Methods for Astrophysics · Physics 2026-02-09 Florian Cheyssial , Laurent Mugnier , Cyril Petit

First multi-conjugate adaptive-optical (MCAO) systems are currently being installed on solar telescopes. The aim of these systems is to increase the corrected field-of-view with respect to conventional adaptive optics. However, this first…

Instrumentation and Methods for Astrophysics · Physics 2015-06-05 Aglae Kellerer

The small correction volume for conventional wavefront shaping methods limits their applications in biological imaging through scattering media. We demonstrate large volume wavefront shaping through a scattering layer with a single…

Biological Physics · Physics 2017-02-20 Xiaodong Tao , Tuwin Lam , Bingzhao Zhu , Qinggele Li , Marc R. Reinig , Joel Kubby

Shack-Hartmann wavefront sensors for both solar and laser guide star adaptive optics (with elongated spots) need to observe extended objects. Correlation techniques have been successfully employed to measure the wavefront gradient in solar…

Instrumentation and Methods for Astrophysics · Physics 2018-09-11 M. J. Townson , G. D Love , C. D. Saunter

Light scattering and aberrations limit optical microscopy in biological tissue, which motivates the development of adaptive optics techniques. Here, we develop a method for adaptive optics with reflected light and deep neural networks…

Optics · Physics 2020-07-28 Ivan Vishniakou , Johannes D. Seelig

The use of laser guide stars in astronomical adaptive optics results in elongated Shack-Hartmann wavefront sensor image patterns. Image correlation techniques can be used to determine local wavefront slope by correlating each sub-aperture…

Instrumentation and Methods for Astrophysics · Physics 2014-03-05 Alastair Basden

Over the past fifty years, wavefront sensing technology has continuously evolved from basic techniques to high-precision systems, serving as a core methodology in adaptive optics (AO). Beyond traditional wavefront retrieval methods based on…

Optics · Physics 2025-05-13 Rundong Fan , Zichao Wang , Pei Li , Lei Huang
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