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Optical imaging of genetically encoded calcium indicators is a powerful tool to record the activity of a large number of neurons simultaneously over a long period of time from freely behaving animals. However, determining the exact time at…

Applications · Statistics 2021-03-08 Tong Shen , Kevin Johnston , Gyorgy Lur , Michele Guindani , Hernando Ombao , Zhaoxia Yu

In recent years, a number of methods have been proposed to estimate the times at which a neuron spikes on the basis of calcium imaging data. However, quantifying the uncertainty associated with these estimated spikes remains an open…

Methodology · Statistics 2026-04-27 Yiqun T. Chen , Sean W. Jewell , Daniela M. Witten

We present efficient Bayesian methods for extracting neuronal spiking information from calcium imaging data. The goal of our methods is to sample from the posterior distribution of spike trains and model parameters (baseline concentration,…

Neurons and Cognition · Quantitative Biology 2013-11-28 Eftychios A. Pnevmatikakis , Josh Merel , Ari Pakman , Liam Paninski

Calcium imaging permits optical measurement of neural activity. Since intracellular calcium concentration is an indirect measurement of neural activity, computational tools are necessary to infer the true underlying spiking activity from…

Machine Learning · Statistics 2017-11-07 Artur Speiser , Jinyao Yan , Evan Archer , Lars Buesing , Srinivas C. Turaga , Jakob H. Macke

Calcium imaging allows for the parallel measurement of large neuronal populations in a spatially resolved and minimally invasive manner, and has become a gold-standard for neuronal functionality. While deep generative models have been…

Neurons and Cognition · Quantitative Biology 2025-10-02 Berta Ros , Mireia Olives-Verger , Caterina Fuses , Josep M Canals , Jordi Soriano , Jordi Abante

For the past few years, we have developed flexible, active, multiplexed recording devices for high resolution recording over large, clinically relevant areas in the brain. While this technology has enabled a much higher-resolution view of…

Neurons and Cognition · Quantitative Biology 2017-06-06 Yilin Song , Jonathan Viventi , Yao Wang

Calcium imaging data promises to transform the field of neuroscience by making it possible to record from large populations of neurons simultaneously. However, determining the exact moment in time at which a neuron spikes, from a calcium…

Methodology · Statistics 2018-02-22 Sean Jewell , Toby Dylan Hocking , Paul Fearnhead , Daniela Witten

Understanding how neurons coordinate their activity is a fundamental question in neuroscience, with implications for learning, memory, and neurological disorders. Calcium imaging has emerged as a powerful method to observe large-scale…

Methodology · Statistics 2026-03-03 Laura D'Angelo , Francesco Denti , Antonio Canale , Michele Guindani

Recent advancements in miniaturized fluorescence microscopy have made it possible to investigate neuronal responses to external stimuli in awake behaving animals through the analysis of intra-cellular calcium signals. An on-going challenge…

Applications · Statistics 2022-01-28 Laura D'Angelo , Antonio Canale , Zhaoxia Yu , Michele Guindani

We introduce a new supervised learning algorithm based to train spiking neural networks for classification. The algorithm overcomes a limitation of existing multi-spike learning methods: it solves the problem of interference between…

Neural and Evolutionary Computing · Computer Science 2021-08-12 Huy Le Nguyen , Dominique Chu

Calcium imaging is an important technique for monitoring the activity of thousands of neurons simultaneously. As calcium imaging datasets grow in size, automated detection of individual neurons is becoming important. Here we apply a…

Neurons and Cognition · Quantitative Biology 2016-12-23 Noah J. Apthorpe , Alexander J. Riordan , Rob E. Aguilar , Jan Homann , Yi Gu , David W. Tank , H. Sebastian Seung

Calcium imaging for observing spiking activity from large populations of neurons are quickly gaining popularity. While the raw data are fluorescence movies, the underlying spike trains are of interest. This work presents a fast non-negative…

Quantitative Methods · Quantitative Biology 2011-02-21 Joshua T. Vogelstein , Adam M. Packer , Tim A. Machado , Tanya Sippy , Baktash Babadi , Rafael Yuste , Liam Paninski

The current article introduces a supervised learning algorithm for multilayer spiking neural networks. The algorithm presented here overcomes some limitations of existing learning algorithms as it can be applied to neurons firing multiple…

Neural and Evolutionary Computing · Computer Science 2014-02-04 Ioana Sporea , André Grüning

With the increasing popularity of calcium imaging data in neuroscience research, methods for analyzing calcium trace data are critical to address various questions. The observed calcium traces are either analyzed directly or deconvolved to…

Neurons and Cognition · Quantitative Biology 2021-03-04 Tong Shen , Gyorgy Lur , Xiangmin Xu , Zhaoxia Yu

We study noisy calcium imaging data, with a focus on the classification of spike traces. As raw traces obscure the true temporal structure of neuron's activity, we performed a tuned filtering of the calcium concentration using two methods:…

Neurons and Cognition · Quantitative Biology 2024-09-17 Arianna Burzacchi , Nicoletta D'Angelo , David Payares-Garcia , Jorge Mateu

Two-photon imaging of calcium indicators allows simultaneous recording of responses of hundreds of neurons over hours and even days, but provides a relatively indirect measure of their spiking activity. Existing deconvolution algorithms…

Neurons and Cognition · Quantitative Biology 2016-01-05 Elad Ganmor , Michael Krumin , Luigi F. Rossi , Matteo Carandini , Eero P. Simoncelli

An optical flow gradient algorithm was applied to spontaneously forming net- works of neurons and glia in culture imaged by fluorescence optical microscopy in order to map functional calcium signaling with single pixel resolution. Optical…

Computational Engineering, Finance, and Science · Computer Science 2010-01-22 Marius Buibas , Diana Yu , Krystal Nizar , Gabriel A. Silva

Experimental studies support the notion of spike-based neuronal information processing in the brain, with neural circuits exhibiting a wide range of temporally-based coding strategies to rapidly and efficiently represent sensory stimuli.…

Neural and Evolutionary Computing · Computer Science 2020-08-18 Brian Gardner , André Grüning

Deducing the structure of neural circuits is one of the central problems of modern neuroscience. Recently-introduced calcium fluorescent imaging methods permit experimentalists to observe network activity in large populations of neurons,…

Applications · Statistics 2011-07-22 Yuriy Mishchencko , Joshua T. Vogelstein , Liam Paninski

In the past few years, new technologies in the field of neuroscience have made it possible to simultaneously image activity in large populations of neurons at cellular resolution in behaving animals. In mid-2016, a huge repository of this…

Applications · Statistics 2017-03-22 Ashley Petersen , Noah Simon , Daniela Witten
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