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The analysis of biological sequencing data has been one of the biggest applications of string algorithms. The approaches used in many such applications are based on the analysis of k-mers, which are short fixed-length strings present in a…

Data Structures and Algorithms · Computer Science 2020-06-15 Rayan Chikhi , Jan Holub , Paul Medvedev

The extraction of $k$-mers is a fundamental component in many complex analyses of large next-generation sequencing datasets, including reads classification in genomics and the characterization of RNA-seq datasets. The extraction of all…

Quantitative Methods · Quantitative Biology 2021-01-19 Diego Santoro , Leonardo Pellegrina , Fabio Vandin

A basic problem in metagenomics is to assign a sequenced read to the correct species in the reference collection. In typical applications in genomic epidemiology and viral metagenomics the reference collection consists of set of species…

Data Structures and Algorithms · Computer Science 2020-06-11 Dustin Cobas , Veli Mäkinen , Massimiliano Rossi

A major challenge in next-generation genome sequencing (NGS) is to assemble massive overlapping short reads that are randomly sampled from DNA fragments. To complete assembling, one needs to finish a fundamental task in many leading…

Genomics · Quantitative Biology 2015-05-26 Yang Li , XifengYan

Next-generation sequencing (NGS) technologies have enabled affordable sequencing of billions of short DNA fragments at high throughput, paving the way for population-scale genomics. Genomics data analytics at this scale requires overcoming…

Databases · Computer Science 2019-10-11 Darryl Ho , Jialin Ding , Sanchit Misra , Nesime Tatbul , Vikram Nathan , Vasimuddin Md , Tim Kraska

Genomic and metagenomic fields, generating huge sets of short genomic sequences, brought their own share of high performance problems. To extract relevant pieces of information from the huge data sets generated by current sequencing…

Data Structures and Algorithms · Computer Science 2016-05-27 Camille Marchet , Antoine Limasset , Lucie Bittner , Pierre Peterlongo

Pairwise alignment of DNA sequencing data is a ubiquitous task in bioinformatics and typically represents a heavy computational burden. State-of-the-art approaches to speed up this task use hashing to identify short segments (k-mers) that…

Machine Learning · Computer Science 2021-02-16 Govinda M. Kamath , Tavor Z. Baharav , Ilan Shomorony

The advent of "next-generation" DNA sequencing (NGS) technologies has meant that collections of hundreds of millions of DNA sequences are now commonplace in bioinformatics. Knowing the longest common prefix array (LCP) of such a collection…

Data Structures and Algorithms · Computer Science 2013-05-02 Markus J. Bauer , Anthony J. Cox , Giovanna Rosone , Marinella Sciortino

Feature embedding methods have been proposed in literature to represent sequences as numeric vectors to be used in some bioinformatics investigations, such as family classification and protein structure prediction. Recent theoretical…

Background: Short sequence substrings of a fixed length k, called k-mers, are a ubiquitous computational primitive in bioinformatics, used across sequence indexing, read mapping, genome assembly, metagenomic classification, and comparative…

Genomics · Quantitative Biology 2026-05-15 Lucas Czech

Motivation: Building the histogram of occurrences of every $k$-symbol long substring of nucleotide data is a standard step in many bioinformatics applications, known under the name of $k$-mer counting. Its applications include developing de…

Data Structures and Algorithms · Computer Science 2017-03-03 Sebastian Deorowicz , Marek Kokot , Szymon Grabowski , Agnieszka Debudaj-Grabysz

Next Generation Sequencing (NGS) technologies generate large amounts of short read data for many different organisms. The fact that NGS reads are generally short makes it challenging to assemble the reads and reconstruct the original genome…

Genomics · Quantitative Biology 2015-04-07 Jie Ren , Kai Song , Minghua Deng , Gesine Reinert , Charles H. Cannon , Fengzhu Sun

The fundamental question considered in algorithms on strings is that of indexing, that is, preprocessing a given string for specific queries. By now we have a number of efficient solutions for this problem when the queries ask for an exact…

Data Structures and Algorithms · Computer Science 2023-04-04 Paweł Gawrychowski , Garance Gourdel , Tatiana Starikovskaya , Teresa Anna Steiner

Aligning millions of short DNA or RNA reads, of 75 to 250 base pairs each, to a reference genome is a significant computation problem in bioinformatics. We present a flexible and fast FPGA-based short read alignment tool. Our aligner makes…

Genomics · Quantitative Biology 2018-05-02 Nathaniel McVicar , Akina Hoshino , Anna La Torre , Thomas A. Reh , Walter L. Ruzzo , Scott Hauck

We propose a frame-based representation of k-mers for detecting sequencing errors and rare variants in next generation sequencing data obtained from populations of closely related genomes. Frames are sets of non-orthogonal basis functions,…

Genomics · Quantitative Biology 2016-04-19 Raunaq Malhotra , Manjari Mukhopadhyay , Mary Poss , Raj Acharya

Brown et al.\ (2025) described a pre-processing step, called $k$-mer based breaking (KeBaB), that speeds up searching for long maximal exact matches (MEMs) between a pattern $P$ and an indexed repetitive text $T$. KeBaB produces a set of…

Data Structures and Algorithms · Computer Science 2026-05-29 Travis Gagie

DNA read mapping is a computationally expensive bioinformatics task, required for genome assembly and consensus polishing. It requires to find the best-fitting location for each DNA read on a long reference sequence. A novel resistive…

Genomics · Quantitative Biology 2019-01-29 Roman Kaplan , Leonid Yavits , Ran Ginosar

The string indexing problem is a fundamental computational problem with numerous applications, including information retrieval and bioinformatics. It aims to efficiently solve the pattern matching problem: given a text T of length n for…

Data Structures and Algorithms · Computer Science 2025-09-03 Waseem Akram , Takuya Mieno

Obtaining effective representations of DNA sequences is crucial for genome analysis. Metagenomic binning, for instance, relies on genome representations to cluster complex mixtures of DNA fragments from biological samples with the aim of…

Machine Learning · Computer Science 2024-11-05 Abdulkadir Celikkanat , Andres R. Masegosa , Thomas D. Nielsen

Similarity search based on a distance function in metric spaces is a fundamental problem for many applications. Queries for similar objects lead to the well-known machine learning task of nearest-neighbours identification. Many data…

Information Retrieval · Computer Science 2022-08-05 Felipe Ortega , Maria Jesus Algar , Isaac Martín de Diego , Javier M. Moguerza
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