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Microscopy is one of the most essential imaging techniques in life sciences. High-quality images are required in order to solve (potentially life-saving) biomedical research problems. Many microscopy techniques do not achieve sufficient…

Computer Vision and Pattern Recognition · Computer Science 2018-10-24 Joris Roels , Jan Aelterman , Jonas De Vylder , Hiep Luong , Yvan Saeys , Wilfried Philips

Light sheet fluorescence microscopy is able to image large specimen with high resolution by imaging the sam- ples from multiple angles. Multi-view deconvolution can significantly improve the resolution and contrast of the images, but its…

Quantitative Methods · Quantitative Biology 2014-12-03 Stephan Preibisch , Fernando Amat , Evangelia Stamataki , Mihail Sarov , Robert H. Singer , Eugene Myers , Pavel Tomancak

The spatial resolution of images of living samples obtained by fluorescence microscopes is physically limited due to the diffraction of visible light, which makes the study of entities of size less than the diffraction barrier (around 200…

Image and Video Processing · Electrical Eng. & Systems 2023-03-21 Vasiliki Stergiopoulou , Subhadip Mukherjee , Luca Calatroni , Laure Blanc-Féraud

We propose a deconvolution algorithm for images blurred and degraded by a Poisson noise. The algorithm uses a fast proximal backward-forward splitting iteration. This iteration minimizes an energy which combines a \textit{non-linear} data…

Applications · Statistics 2008-12-18 François-Xavier Dupé , Jalal Fadili , Jean Luc Starck

Due to image blurring image deconvolution is often used for studying biological structures in fluorescence microscopy. Fluorescence microscopy image volumes inherently suffer from intensity inhomogeneity, blur, and are corrupted by various…

Computer Vision and Pattern Recognition · Computer Science 2019-07-15 Soonam Lee , Shuo Han , Paul Salama , Kenneth W. Dunn , Edward J. Delp

Lensfree on-chip microscopy is an emerging imaging technique that can be used to visualize and study biological specimens without the need for imaging lens systems. Important issues that can limit the performance of lensfree on-chip…

Optics · Physics 2015-05-11 Alexander Wong , Farnoud Kazemzadeh , Chao Jin , Xiao Yu Wang

Natural images are often affected by random noise and image denoising has long been a central topic in Computer Vision. Many algorithms have been introduced to remove the noise from the natural images, such as Gaussian, Wiener filtering and…

Computer Vision and Pattern Recognition · Computer Science 2013-08-07 Hyuntaek Oh

In this paper, a methodology is investigated for signal recovery in the presence of non-Gaussian noise. In contrast with regularized minimization approaches often adopted in the literature, in our algorithm the regularization parameter is…

Optimization and Control · Mathematics 2017-01-23 Yosra Marnissi , Yuling Zheng , Emilie Chouzenoux , Jean-Christophe Pesquet

Confocal microscopy is essential for histopathologic cell visualization and quantification. Despite its significant role in biology, fluorescence confocal microscopy suffers from the presence of inherent noise during image acquisition.…

Image and Video Processing · Electrical Eng. & Systems 2020-05-28 Saeed Izadi , Ghassan Hamarneh

Many microscopy applications are limited by the total amount of usable light and are consequently challenged by the resulting levels of noise in the acquired images. This problem is often addressed via (supervised) deep learning based…

Image and Video Processing · Electrical Eng. & Systems 2020-08-20 Anna S. Goncharova , Alf Honigmann , Florian Jug , Alexander Krull

Adherent biological cells generate traction forces on a substrate that play a central role for migration, mechanosensing, differentiation, and collective behavior. The established method for quantifying this cell-substrate interaction is…

Cell Behavior · Quantitative Biology 2020-05-05 Yunfei Huang , Gerhard Gompper , Benedikt Sabass

In this paper, we propose a Bayesian spectral deconvolution method for absorption spectra. In conventional analysis, the noise mechanism of absorption spectral data is never considered appropriately. In that analysis, the least-squares…

Methodology · Statistics 2023-04-21 Tomohiro Nabika , Kenji Nagata , Shun Katakami , Masaichiro Mizumaki , Masato Okada

Fluoroscopy is an imaging technique that uses X-ray to obtain a real-time 2D video of the interior of a 3D object, helping surgeons to observe pathological structures and tissue functions especially during intervention. However, it suffers…

Image and Video Processing · Electrical Eng. & Systems 2022-08-31 Ruizhou Liu , Qiang Ma , Zhiwei Cheng , Yuanyuan Lyu , Jianji Wang , S. Kevin Zhou

In multi-photon microscopy (MPM), a recent in-vivo fluorescence microscopy system, the task of image restoration can be decomposed into two interlinked inverse problems: firstly, the characterization of the Point Spread Function (PSF) and…

Image and Video Processing · Electrical Eng. & Systems 2024-11-08 Julien Ajdenbaum , Emilie Chouzenoux , Claire Lefort , Ségolène Martin , Jean-Christophe Pesquet

We present a novel, general-purpose method for deconvolving and denoising images from gridded radio interferometric visibilities using Bayesian inference based on a Gaussian process model. The method automatically takes into account…

Instrumentation and Methods for Astrophysics · Physics 2015-06-17 P. M. Sutter , Benjamin D. Wandelt , Jason D. McEwen , Emory F. Bunn , Ata Karakci , Andrei Korotkov , Peter Timbie , Gregory S. Tucker , Le Zhang

Synapses are densely packed submicron structures that dynamically reorganize during learning and memory formation. Longitudinal \textit{in vivo} imaging of fluorescently tagged synaptic receptors offers a promising opportunity to study…

Computer Vision and Pattern Recognition · Computer Science 2026-05-27 Shashwat Kumar , Dominic M. Padova , Binish Narang , Gabrielle I. Coste , Austin R. Graves , Richard L. Huganir , Adam S. Charles , Michael I. Miller , Anuj Srivastava

This work proposes a learning-based statistical refinement method for improving the denoising results of a given denoiser without knowing the precise noise distribution or accessing clean images or calibration data. While there are many…

Machine Learning · Computer Science 2026-05-07 Rihuan Ke

Fluoroscopy is critical for real-time X-ray visualization in medical imaging. However, low-dose images are compromised by noise, potentially affecting diagnostic accuracy. Noise reduction is crucial for maintaining image quality, especially…

Image and Video Processing · Electrical Eng. & Systems 2024-11-05 Sun-Young Jeon , Sen Wang , Adam S. Wang , Garry E. Gold , Jang-Hwan Choi

Image denoising is an important pre-processing step in medical image analysis. Different algorithms have been proposed in past three decades with varying denoising performances. More recently, having outperformed all conventional methods,…

Computer Vision and Pattern Recognition · Computer Science 2017-02-21 Lovedeep Gondara

Low fluence illumination sources can facilitate clinical transition of photoacoustic imaging because they are rugged, portable, affordable, and safe. However, these sources also decrease image quality due to their low fluence. Here, we…

Image and Video Processing · Electrical Eng. & Systems 2020-04-21 Ali Hariri , Kamran Alipour , Yash Mantri , Jurgen P. Schulze , Jesse V. Jokerst
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