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This comprehensive review discusses the development of scanning electron microscopy and the application of this technology in different fields such as biology, nanobiotechnology and biomedical science. Besides being a tool for high…

Quantitative Methods · Quantitative Biology 2023-11-02 Aniruddha Acharya

Quantitative microstructural characterization is fundamental to materials science, where electron micrograph (EM) provides indispensable high-resolution insights. However, progress in deep learning-based EM characterization has been…

Computer Vision and Pattern Recognition · Computer Science 2025-08-25 Nan wang , Zhiyi Xia , Yiming Li , Shi Tang , Zuxin Fan , Xi Fang , Haoyi Tao , Xiaochen Cai , Guolin Ke , Linfeng Zhang , Yanhui Hong

Cryogenic Electron Tomography (CryoET) combined with sub-volume averaging (SVA) is the only imaging modality capable of resolving protein structures inside cells at molecular resolution. Particle picking, the task of localizing and…

Computer Vision and Pattern Recognition · Computer Science 2026-01-14 Linhan Wang , Jianwen Dou , Wang Li , Shengkun Wang , Zhiwu Xie , Chang-Tien Lu , Yinlin Chen

Cryo-electron microscopy (cryo-EM) has emerged as a powerful technique for determining the three-dimensional structures of biological molecules at near-atomic resolution. However, reconstructing helical assemblies presents unique challenges…

Quantitative Methods · Quantitative Biology 2026-03-16 Guy Shapira , Yoel Shkolnisky

Constructing atomic models from cryo-electron microscopy (cryo-EM) maps is a crucial yet intricate task in structural biology. While advancements in deep learning, such as convolutional neural networks (CNNs) and graph neural networks…

Quantitative Methods · Quantitative Biology 2024-11-01 Xin , Ma , Dong Si

The group synchronization problem involves estimating a collection of group elements from noisy measurements of their pairwise ratios. This task is a key component in many computational problems, including the molecular reconstruction…

Signal Processing · Electrical Eng. & Systems 2022-12-09 Noam Janco , Tamir Bendory

This dissertation explores how deep generative models can advance the analysis of challenging biological problems by integrating domain knowledge with deep learning. It focuses on two areas: DNA reaction kinetics and cryogenic electron…

Machine Learning · Computer Science 2026-05-07 Chenwei Zhang

Quantitative high-resolution transmission electron microscopy (HRTEM) provides an indispensable means to understand the structure-property relationships of a material in atomic dimensions. Successful quantification requires reliable…

Computational Physics · Physics 2026-03-27 Xiankang Tang , Yixuan Zhang , Juri Barthel , Chun-Lin Jia , Rafal E. Dunin-Borkowski , Hongbin Zhang , Lei Jin

Achieving a comprehensive understanding of the behaviour of proteins is greatly facilitated by the knowledge of their structures, thermodynamics and dynamics. All this information can be provided in an effective manner in terms of…

Quantitative Methods · Quantitative Biology 2019-03-27 Massimiliano Bonomi , Michele Vendruscolo

We propose a method to reconstruct the 3-D molecular structure from micrographs collected at just one sample tilt angle in the random conical tilt scheme in cryo-electron microscopy. Our method uses autocorrelation analysis on the…

Data Analysis, Statistics and Probability · Physics 2021-01-12 Ti-Yen Lan , Nicolas Boumal , Amit Singer

Cryogenic Electron Tomography (CryoET) is a useful imaging technology in structural biology that is hindered by its need for manual annotations, especially in particle picking. Recent works have endeavored to remedy this issue with few-shot…

Quantitative Methods · Quantitative Biology 2024-07-10 Yizhou Zhao , Hengwei Bian , Michael Mu , Mostofa R. Uddin , Zhenyang Li , Xiang Li , Tianyang Wang , Min Xu

Electron microscopy has enabled many scientific breakthroughs across multiple fields. A key challenge is the tuning of microscope parameters based on images to overcome optical aberrations that deteriorate image quality. This calibration…

Computer Vision and Pattern Recognition · Computer Science 2026-03-20 Jilles S. van Hulst , W. P. M. H. Heemels , Duarte J. Antunes

The CryoEM single particle imaging method has recently received broad attention in the field of structural biology for determining the structures of biological molecules. The structures can be resolved to near-atomic resolutions after…

Biomolecules · Quantitative Biology 2017-12-27 Yihua Wang , Daqi Yu , Qi Ouyang , Haiguang Liu

Cellular Electron CryoTomography (CECT) is a 3D imaging technique that captures information about the structure and spatial organization of macromolecular complexes within single cells, in near-native state and at sub-molecular resolution.…

Quantitative Methods · Quantitative Biology 2018-07-04 Kai Wen Wang , Xiangrui Zeng , Xiaodan Liang , Zhiguang Huo , Eric P. Xing , Min Xu

We present initial results on the use of Mixture Models for density estimation in large astronomical databases. We provide herein both the theoretical and experimental background for using a mixture model of Gaussians based on the…

Astrophysics · Physics 2007-05-23 R. C. Nichol , A. J. Connolly , A. W. Moore , J. Schneider , C. Genovese , L. Wasserman

Cryo-electron tomography (cryo-ET) enables 3D visualization of cellular structures. Accurate reconstruction of high-resolution volumes is complicated by the very low signal-to-noise ratio and a restricted range of sample tilts. Recent…

Image and Video Processing · Electrical Eng. & Systems 2026-03-03 Vinith Kishore , Valentin Debarnot , AmirEhsan Khorashadizadeh , Ricardo D. Righetto , Benjamin D. Engel , Ivan Dokmanić

Cryo-electron microscopy is a state-of-the-art method for determining high-resolution three-dimensional models of molecules, from their two-dimensional projection images taken by an electron microscope. A crucial step in this method is to…

Image and Video Processing · Electrical Eng. & Systems 2022-12-16 Eitan Rosen , Yoel Shkolnisky

We apply a recently developed framework for analyzing the convergence of stochastic algorithms to the general problem of large-scale nonconvex composite optimization more generally, and nonconvex likelihood maximization in particular. Our…

Optimization and Control · Mathematics 2024-01-25 D. Russell Luke , Steffen Schultze , Helmut Grubmüller

Cryo-ET allows to generate tomograms of biological samples in situ, capturing complex structures in their native context. Despite low signal-to-noise ratio in reconstructed volumes, the large number of copies of the same macromolecules…

Signal Processing · Electrical Eng. & Systems 2025-09-03 Fabian Kruse , Vinith Kishore , Valentin Debarnot , Ivan Dokmanić

We study a class of orbit recovery problems in which we observe independent copies of an unknown element of $\mathbb{R}^p$, each linearly acted upon by a random element of some group (such as $\mathbb{Z}/p$ or $\mathrm{SO}(3)$) and then…

Statistics Theory · Mathematics 2023-06-26 Afonso S. Bandeira , Ben Blum-Smith , Joe Kileel , Amelia Perry , Jonathan Niles-Weed , Alexander S. Wein
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