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Protein-nucleic acid complexes are important for many cellular processes including the most essential function such as transcription and translation. For many protein-nucleic acid complexes, flexibility of both macromolecules has been shown…

Biomolecules · Quantitative Biology 2015-10-28 Kristopher Opron , Kelin Xia , Zachary F. Burton , Guo-Wei Wei

Flexibility-rigidity index (FRI) has been developed as a robust, accurate and efficient method for macromolecular thermal fluctuation analysis and B-factor prediction. The performance of FRI depends on its formulations of rigidity index and…

Biomolecules · Quantitative Biology 2016-09-15 Duc Duy Nguyen , Kelin Xia , Guo-Wei Wei

Existing elastic network models are typically parametrized at a given cutoff distance and often fail to properly predict the thermal fluctuation of many macromolecules that involve multiple characteristic length scales. We introduce a…

Biomolecules · Quantitative Biology 2015-05-21 Kristopher Opron , Kelin Xia , Guo-Wei Wei

Gaussian network model(GNM) and anisotropic network model(ANM) are some of the most popular methods for the study of protein flexibility and related functions. In this work, we propose generalized GNM(gGNM) and ANM methods and show that the…

Biomolecules · Quantitative Biology 2016-01-20 Kelin Xia , Kristopher Opron , Guo-Wei Wei

Flexibility is an intrinsic essential feature of protein structures, directly linked to their functions. To this day, most of the prediction methods use the crystallographic data (namely B-factors) as the only indicator of protein's inner…

Protein rigidity and flexibility can be analyzed accurately and efficiently using the program FIRST. Previous studies using FIRST were designed to analyze the rigidity and flexibility of proteins using a single static (snapshot) structure.…

Biomolecules · Quantitative Biology 2015-06-15 Adnan Sljoka , Derek Wilson

Most existing feature selection methods are insufficient for analytic purposes as soon as high dimensional data or redundant sensor signals are dealt with since features can be selected due to spurious effects or correlations rather than…

Machine Learning · Computer Science 2019-08-13 Lukas Pfannschmidt , Christina Göpfert , Ursula Neumann , Dominik Heider , Barbara Hammer

When described by a low-dimensional reaction coordinate, the rates of protein folding are determined by a subtle interplay between free-energy barriers and friction. While it is commonplace to extract free-energy profiles from molecular…

Soft Condensed Matter · Physics 2022-10-24 Benjamin A. Dalton , Cihan Ayaz , Lucas Tepper , Roland R. Netz

Normal mode analysis offers an efficient way of modeling the conformational flexibility of protein structures. Simple models defined by contact topology, known as elastic network models, have been used to model a variety of systems, but the…

Biomolecules · Quantitative Biology 2007-05-23 Dmitry A. Kondrashov , Adam W. Van Wynsberghe , Ryan M. Bannen , Qiang Cui , George N. Phillips

Protein dynamics play a crucial role in many biological processes and drug interactions. However, measuring, and simulating protein dynamics is challenging and time-consuming. While machine learning holds promise in deciphering the…

Machine Learning · Computer Science 2024-08-23 Sina Sarparast , Aldo Zaimi , Maximilian Ebert , Michael-Rock Goldsmith

Cryo-electron microscopy (cryo-EM) is a technique for reconstructing the 3-dimensional (3D) structure of biomolecules (especially large protein complexes and molecular assemblies). As the resolution increases to the near-atomic scale,…

Computer Vision and Pattern Recognition · Computer Science 2023-08-08 Weijie Chen , Xinyan Wang , Yuhang Wang

Predicting the three-dimensional (3D) structure of a protein from its primary sequence of amino acids is known as the protein folding (PF) problem. Due to the central role of proteins' 3D structures in chemistry, biology and medicine…

Quantum Physics · Physics 2021-03-18 Anton Robert , Panagiotis Kl. Barkoutsos , Stefan Woerner , Ivano Tavernelli

AlphaFold 3 (AF3), the latest version of protein structure prediction software, goes beyond its predecessors by predicting protein-protein complexes. It could revolutionize drug discovery and protein engineering, marking a major step…

Biomolecules · Quantitative Biology 2024-06-07 JunJie Wee , Guo-Wei Wei

The rigidity and flexibility of homologous psychrophilic(P), mesophilic(M) and thermophilic(T) proteins have been investigated at the global and local levels in terms of packing factor and atomic fluctuations obtained from B-factors. For…

Biological Physics · Physics 2021-06-08 Srikanta Sen , Munna Sarkar

Recent advances in geometric deep learning and generative modeling have enabled the design of novel proteins with a wide range of desired properties. However, current state-of-the-art approaches are typically restricted to generating…

Biomolecules · Quantitative Biology 2025-08-26 Vsevolod Viliuga , Leif Seute , Nicolas Wolf , Simon Wagner , Arne Elofsson , Jan Stühmer , Frauke Gräter

Protein flexibility is crucial for understanding protein structures, functions, and dynamics, and it can be measured through experimental methods such as X-ray crystallography. Theoretical approaches have also been developed to predict…

Biomolecules · Quantitative Biology 2024-11-06 Hongsong Feng , Jeffrey Y. Zhao , Guo-Wei Wei

Protein-protein interactions (protein functionalities) are mediated by water, which compacts individual proteins and promotes close and temporarily stable large-area protein-protein interfaces. In their classic paper Kyte and Doolittle (KD)…

Soft Condensed Matter · Physics 2009-11-13 Alexander E. Kister , James C. Phillips

Magnetic resonance imaging (MRI) is the non-invasive modality of choice for body tissue composition analysis due to its excellent soft tissue contrast and lack of ionizing radiation. However, quantification of body composition requires an…

Computer Vision and Pattern Recognition · Computer Science 2018-10-16 Ismail Irmakci , Sarfaraz Hussein , Aydogan Savran , Rita R. Kalyani , David Reiter , Chee W. Chia , Kenneth W. Fishbein , Richard G. Spencer , Luigi Ferrucci , Ulas Bagci

The increasing number of protein-based metamaterials demands reliable and efficient theoretical and computational methods to study the physicochemical properties they may display. In this regard, we develop a simulation strategy based on…

Soft Condensed Matter · Physics 2020-06-23 J. A. Campos Gonzalez Angulo , G. Wiesehan , R. F. Ribeiro , J. Yuen-Zhou

Determining the folding core of a protein yields information about its folding process and dynamics. The experimental procedures for identifying the amino acids which make up the folding core include hydrogen-deuterium exchange and…

Biomolecules · Quantitative Biology 2015-04-09 J. W. Heal , S. A. Wells , R. B. Freedman , R. A. Römer
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