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Single-stranded RNA viruses efficiently encapsulate their genome into a protein shell called the capsid. Electrostatic interactions between the positive charges in the capsid protein's N-terminal tail and the negatively charged genome have…

Biological Physics · Physics 2021-02-03 Yinan Dong , Siyu Li , Roya Zandi

The mechanical properties of DNA play a critical role in many biological functions. For example, DNA packing in viruses involves confining the viral genome in a volume (the viral capsid) with dimensions that are comparable to the DNA…

RNA viruses are a widely used tool to study evolution experimentally. Many standard protocols of virus propagation and competition are done at nominally low multiplicity of infection (m.o.i.), but lead during one passage to two or more…

Populations and Evolution · Quantitative Biology 2007-05-23 Claus O. Wilke , Daniel D. Reissig , Isabel S. Novella

Weak purifying selection, acting on many linked mutations, may play a major role in shaping patterns of molecular evolution in natural populations. Yet efforts to infer these effects from DNA sequence data are limited by our incomplete…

Populations and Evolution · Quantitative Biology 2012-10-17 Benjamin H Good , Michael M Desai

Background: The rate at which fitness declines as an organism's genome accumulates random mutations is an important variable in several evolutionary theories. At an intuitive level, it might seem natural that random mutations should tend to…

Biological Physics · Physics 2007-05-23 Claus O Wilke , Richard E Lenski , Christoph Adami

In addition to variation in terms of single nucleotide polymorphisms (SNPs), whole regions ranging from several kilobases up to a megabase in length differ in copy number among individuals. These differences are referred to as Copy Number…

Genomics · Quantitative Biology 2007-09-27 Philip M. Kim , Jan O. Korbel , Xueying Chen , Mark B. Gerstein

The standard genetic code is known to be robust to translation errors and point mutations. We studied how small modifications of the standard code affect its robustness. The robustness was assessed in terms of a proper stability function,…

Quantitative Methods · Quantitative Biology 2009-09-25 V. R. Chechetkin , V. V. Lobzin

The study of genomic variation has provided key insights into the functional role of mutations. Predominantly, studies have focused on single nucleotide variants (SNV), which are relatively easy to detect and can be described with rich…

Genomics · Quantitative Biology 2015-09-04 Daniel R. Zerbino , Tracy Ballinger , Benedict Paten , Glenn Hickey , David Haussler

Most of the DNA that composes a complex organism is non-coding and defined as junk. Even the coding part is composed of genes that affect the phenotype differently. Therefore, a random mutation has an effect on the specimen fitness that…

Populations and Evolution · Quantitative Biology 2021-07-19 Mattia Miotto , Lorenzo Monacelli

The macromolecules that encode and translate information in living systems, DNA and RNA, exhibit distinctive structural asymmetries, including homochirality or mirror image asymmetry and $3' - 5'$ directionality, that are invariant across…

Biomolecules · Quantitative Biology 2017-03-10 Hemachander Subramanian , Robert A. Gatenby

Viruses display striking diversity in structure, transmission mode, immune interaction, and evolutionary behavior. Despite this diversity, viral strategies are not unconstrained. Here we present a unifying framework that treats viral…

Biological Physics · Physics 2025-12-30 Mohammad Rasoolinejad

RNA virus populations will undergo processes of mutation and selection resulting in a mixed population of viral particles. High throughput sequencing of a viral population subsequently contains a mixed signal of the underlying clones. We…

Populations and Evolution · Quantitative Biology 2014-07-31 Donatien Fotso-Chedom , Pablo R. Murcia , Chris D. Greenman

The statistical mechanics of a treelike polymer in a confining volume is relevant to the packaging of the genome in RNA viruses. Making use of the mapping of the grand partition function of this system onto the statistical mechanics of a…

Statistical Mechanics · Physics 2008-04-09 Rouzbeh Ghafouri , Joseph Rudnick , Robijn Bruinsma

RNA viruses exist in large intra-host populations which display great genotypic and phenotypic diversity. We analyze a model of viral competition between two different viral strains infecting a constantly replenished cell pool, in which we…

Populations and Evolution · Quantitative Biology 2011-01-18 Edgar Delgado-Eckert , Samuel Ojosnegros , Niko Beerenwinkel

We simulate the assembly dynamics of icosahedral capsids from subunits that interconvert between different conformations (or quasi-equivalent states). The simulations identify mechanisms by which subunits form empty capsids with only one…

Biomolecules · Quantitative Biology 2009-09-29 Oren M. Elrad , Michael F. Hagan

Viruses self-assemble from identical capsid proteins and their genome consisting, for example, of a long single stranded (ss) RNA. For a big class of T = 3 viruses capsid proteins have long positive N-terminal tails. We explore the role…

Biomolecules · Quantitative Biology 2015-06-26 Tao Hu , Rui Zhang , B. I. Shklovskii

A lattice model of RNA denaturation which fully accounts for the excluded volume effects among nucleotides is proposed. A numerical study shows that interactions forming pseudoknots must be included in order to get a sharp continuous…

Soft Condensed Matter · Physics 2007-05-23 M. Baiesi , E. Orlandini , A. L. Stella

Electrostatic interactions play a central role in the assembly of single-stranded RNA viruses. Under physiological conditions of salinity and acidity, virus capsid assembly requires the presence of genomic material that is oppositely…

Biomolecules · Quantitative Biology 2009-11-10 P. van der Schoot , R. Bruinsma

Genome length varies widely among organisms, from compact genomes of prokaryotes to vast and complex genomes of eukaryotes. In this study, we theoretically identify the evolutionary pressures that may have driven this divergence in genome…

Genomics · Quantitative Biology 2025-03-03 Parthasarathi Sahu , Sashikanta Barik , Koushik Ghosh , Hemachander Subramanian

Antigenic escape constitutes the main mechanism allowing rapidly evolving viruses to achieve endemicity. Beyond granting immune escape, empirical evidence also suggests that mutations of viruses might increase their inter-host…

Populations and Evolution · Quantitative Biology 2025-11-20 David Soriano-Paños