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The analysis of differential gene expression from RNA-Seq data has become a standard for several research areas mainly involving bioinformatics. The steps for the computational analysis of these data include many data types and file…

Genomics · Quantitative Biology 2021-09-09 Juliana Costa-Silva , Douglas S. Domingues , David Menotti , Mariangela Hungria , Fabricio M Lopes

Substantial statistical research has recently been devoted to the analysis of large-scale microarray experiments which provide a measure of the simultaneous expression of thousands of genes in a particular condition. A typical goal is the…

Methodology · Statistics 2009-10-08 Shane T. Jensen , Ibrahim Erkan , Erna S. Arnardottir , Dylan S. Small

Background: Trace quantities of contaminating DNA are widespread in the laboratory environment, but their presence has received little attention in the context of high throughput sequencing. This issue is highlighted by recent works that…

Genomics · Quantitative Biology 2015-06-18 Richard W Lusk

A Bayesian analysis of the probability of a signal in the presence of background is developed, and criteria are proposed for claiming evidence for, or the discovery of a signal. The method is general and in particular applicable to sparsely…

Data Analysis, Statistics and Probability · Physics 2009-11-13 Allen Caldwell , Kevin Kröninger

Gene expression microarray technologies provide the simultaneous measurements of a large number of genes. Typical analyses of such data focus on the individual genes, but recent work has demonstrated that evaluating changes in expression…

Applications · Statistics 2010-06-29 Babak Shahbaba , Robert Tibshirani , Catherine M. Shachaf , Sylvia K. Plevritis

This paper studies the problem of high-dimensional multiple testing and sparse recovery from the perspective of sequential analysis. In this setting, the probability of error is a function of the dimension of the problem. A simple…

Statistics Theory · Mathematics 2011-06-06 Matthew Malloy , Robert Nowak

In Bayesian accelerated life testing, the most used tool for model comparison is the deviance information criterion. An alternative and more formal approach is to use Bayes factors to compare models. However, Bayesian accelerated life…

Methodology · Statistics 2026-01-16 Neill Smit , Lizanne Raubenheimer

Differential expression (DE) plays a fundamental role toward illuminating the molecular mechanisms driving a difference between groups (e.g., due to treatment or disease). While any analysis is run on particular cells/samples, the intent is…

Quantitative Methods · Quantitative Biology 2023-12-12 Miriam Shiffman , Ryan Giordano , Tamara Broderick

The RNA-sequencing (RNA-seq) is becoming increasingly popular for quantifying gene expression levels. Since the RNA-seq measurements are relative in nature, between-sample normalization of counts is an essential step in differential…

Methodology · Statistics 2016-10-14 Kefei Liu , Jieping Ye , Yang Yang , Li Shen , Hui Jiang

Genome-wide gene expression profiles, as measured with microarrays or RNA-Seq experiments, have revolutionized biological and biomedical research by providing a quantitative measure of the entire mRNA transcriptome. Typically, researchers…

Applications · Statistics 2013-08-01 Neil R. Clark , Kevin Hu , Edward Y. Chen , Qioanan Duan , Avi Ma`ayan

Identifying differentially expressed (DE) genes associated with a sample characteristic is the primary objective of many microarray studies. As more and more studies are carried out with observational rather than well controlled…

Applications · Statistics 2012-07-02 Xi Kathy Zhou , Fei Liu , Andrew J. Dannenberg

In many transcriptomic studies, the correlation of genes might fluctuate with quantitative factors such as genetic ancestry. We propose a method that models the covariance between two variables to vary against a continuous covariate. For…

Methodology · Statistics 2021-05-03 Tae Hyun Kim , Dan Nicolae

The detection of differentially expressed (DE) genes is one of the most commonly studied problems in bioinformatics. For example, the identification of DE genes between distinct disease phenotypes is an important first step in understanding…

Methodology · Statistics 2013-07-31 S. K. Ng , G. J. McLachlan , K. Wang , Z. Nagymanyoki , S. Liu , S. -W. Ng

1. Bayesian inference is difficult because it often requires time consuming tuning of samplers. Differential evolution Monte-Carlo (DEMC) is a self-tuning multi-chain sampling approach which requires minimal input from the operator as…

Methodology · Statistics 2022-09-22 Willem Bonnaffé

DNA microarray experiments, a well-established experimental technique, aim at understanding the function of genes in some biological processes. One of the most common experiments in functional genomics research is to compare two groups of…

Methodology · Statistics 2007-08-22 Javier Cabrera , Ching-Ray Yu

Evaluating modern machine learning models has become prohibitively expensive. Benchmarks such as LMMs-Eval and HELM demand thousands of GPU hours per model. Costly evaluation reduces inclusivity, slows the cycle of innovation, and worsens…

Machine Learning · Computer Science 2026-03-03 Alexander Rubinstein , Benjamin Raible , Martin Gubri , Seong Joon Oh

High-throughput RNA-sequencing (RNA-seq) technologies are powerful tools for understanding cellular state. Often it is of interest to quantify and summarize changes in cell state that occur between experimental or biological conditions.…

Methodology · Statistics 2021-02-16 Andrew Jones , F. William Townes , Didong Li , Barbara E. Engelhardt

Differential gene expression (DGE) analysis is foundational to transcriptomic research, yet tool selection can substantially influence results. This study presents a comprehensive comparison of two widely used DGE tools, edgeR and DESeq2,…

Genomics · Quantitative Biology 2026-05-20 Mostafa Rezapour

RNA-seq has become a de facto standard for measuring gene expression. Traditionally, RNA-seq experiments are mathematically averaged -- they sequence the mRNA of individuals from different treatment groups, hoping to correlate phenotype…

Quantitative Methods · Quantitative Biology 2013-09-05 Surojit Biswas , Yash N. Agrawal , Tatiana S. Mucyn , Jeffery L. Dangl , Corbin D. Jones

Given data obtained under two sampling conditions, it is often of interest to identify variables that behave differently in one condition than in the other. We introduce a method for differential analysis of second-order behavior called…

Methodology · Statistics 2016-02-26 Kelly Bodwin , Kai Zhang , Andrew Nobel