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Super-resolution optical microscopy is a rapidly evolving scientific field dedicated to imaging sub-wavelength sized objects, leaving its mark in multiple branches of biology and technology. While several super-resolution optical microscopy…

Fluorescence microscopy is indispensable in nanoscience and biological sciences. The versatility of labeling target structures with fluorescent dyes permits to visualize structure and function at a subcellular resolution with a wide field…

Super-resolution microscopy has revolutionized optical fluorescence imaging by improving 3D resolution by 1-2 orders of magnitude. While different methods can successfully increase the resolution, all methods share significant differences…

Biological Physics · Physics 2015-01-26 Thomas Pengo , Nicolas Olivier , Suliana Manley

The ability to resolve detail in the object that is being imaged, named by resolution, is the core parameter of an imaging system. Super-resolution is a class of techniques that can enhance the resolution of an imaging system and even…

Data Structures and Algorithms · Computer Science 2022-10-13 Yaonan Jin , Daogao Liu , Zhao Song

High peak power ultrafast lasers are widely used in nonlinear spectroscopy but often limit its spectral resolution because of the broad frequency bandwidth of ultrashort laser pulses. Improving the resolution by achieving spectrally narrow…

Optics · Physics 2009-11-13 Stanislav O. Konorov , Xiaoji G. Xu , John W. Hepburn , Valery Milner

We develop an imaging algorithm that exploits strong scattering to achieve super-resolution in changing random media. The method processes large and diverse array datasets using sparse dictionary learning, clustering, and multidimensional…

Single molecule localization microscopy (SMLM) techniques enable imaging biological samples well beyond the diffraction limit of light, but they vary significantly in their spatial and temporal resolutions. High-order statistical analysis…

Biological Physics · Physics 2016-03-15 Shachar Schidorsky , Xiyu Yi , Yair Razvag , Yonatan Golan , Shimon Weiss , Eilon Sherman

In coherent diffractive imaging (CDI) the resolution of the reconstructed object is limited by the numerical aperture of the experimental setup. We present here a theoretical and numerical study for achieving super-resolution by…

Data Analysis, Statistics and Probability · Physics 2016-09-20 Tatiana Latychevskaia , Yuriy Chushkin , Hans-Werner Fink

Abbe's resolution limit, one of the best-known physical limitations, poses a great challenge for any wave systems in imaging, wave transport, and dynamics. Originally formulated in linear optics, this Abbe's limit can be broken using…

To overcome the physical barriers caused by light diffraction, super-resolution techniques are often applied in fluorescence microscopy. State-of-the-art approaches require specific and often demanding acquisition conditions to achieve…

Super-resolution imaging refers to imaging techniques that surpass the Rayleigh resolution limit. One standard way to achieve super-resolution is by structuring the phase of the field illuminating the object. Although super-resolution…

Applied Physics · Physics 2025-11-13 Nilakshi Senapati , Abhinandan Bhattacharjee , Kedar Khare , Anand K Jha

Terahertz microscopy has attracted attention owing to distinctive characteristics of the THz frequency region, particularly non-ionizing photon energy, spectral fingerprint, and transparency to most nonpolar materials. Nevertheless, the…

Atmospheric turbulence is a serious problem for traditional optical imaging, especially for satellite and aircraft-to-ground imaging. Here, we report a novel and practical phenomenon in which turbulence-free images can be reconstructed on a…

Image and Video Processing · Electrical Eng. & Systems 2021-09-10 Deyang Duan , Yunjie Xia

Optical stellar interferometers have demonstrated milli-arcsecond resolution with few apertures spaced hundreds of meters apart. To obtain rich direct images, many apertures will be needed, for a better sampling of the incoming wavefront.…

Instrumentation and Methods for Astrophysics · Physics 2014-09-17 Arun Surya , Swapan K. Saha , Antoine Labeyrie

The superposition of several optical beams with large mutual angles results in sub-micrometer periodic patterns with a complex intensity, phase and polarization structure. For high-resolution imaging thereof, one often employs optical…

Optics · Physics 2022-12-20 Lipeng Wan , Tianbao Yu , Daomu Zhao , Wolfgang Löffler

The classical bound on image resolution defined by the Rayleigh limit can be beaten by exploiting the properties of quantum mechanical entanglement. If entangled photons are used as signal states, the best possible resolution is instead…

Quantum Physics · Physics 2009-11-13 G. Gilbert , M. Hamrick , Y. S. Weinstein , S. P. Pappas , A. Donadio

The optical resolution of a digital camera is one of its most crucial parameters with broad relevance for consumer electronics, surveillance systems, remote sensing, or medical imaging. However, resolution is physically limited by the…

Computer Vision and Pattern Recognition · Computer Science 2018-12-27 Thomas Köhler

Rayleigh's criterion for resolving two incoherent point sources has been the most influential measure of optical imaging resolution for over a century. In the context of statistical image processing, violation of the criterion is especially…

Quantum Physics · Physics 2016-09-07 Mankei Tsang , Ranjith Nair , Xiao-Ming Lu

Fluorescence imaging is an essential diagnostic tool in many fields, but diffraction-limited optical imaging at depth is limited by scattering. Here, we present a method based on multiple random illuminations, combined with a computational…

Optics · Physics 2026-03-30 Lei Zhu , Tengfei Wu , Bernhard Rauer , Hilton B. de Aguiar , Sylvain Gigan

In this tutorial, we aim to directly recreate some of our "aha" moments when exploring the impact of heat diffusion on the spatial resolution limit of photothermal imaging. Our objective is also to communicate how this physical limit can…

Numerical Analysis · Mathematics 2023-10-24 Peter Burgholzer , Johannes Bauer-Marschallinger , Mike Hettich , Markus Haltmeier