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Related papers: Automatic Annotation of Axoplasmic Reticula in Pur…

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In this paper, we present a new pipeline which automatically identifies and annotates axoplasmic reticula, which are small subcellular structures present only in axons. We run our algorithm on the Kasthuri11 dataset, which was color…

Reconstructing neuronal circuits at the level of synapses is a central problem in neuroscience and becoming a focus of the emerging field of connectomics. To date, electron microscopy (EM) is the most proven technique for identifying and…

Quantitative Methods · Quantitative Biology 2014-12-05 Stephen M. Plaza , Toufiq Parag , Gary B. Huang , Donald J. Olbris , Mathew A. Saunders , Patricia K. Rivlin

Producing connectomes from electron microscopy (EM) images has historically required a great deal of human proofreading effort. This manual annotation cost is the current bottleneck in scaling EM connectomics, for example, in making larger…

Computer Vision and Pattern Recognition · Computer Science 2025-10-01 Gary B Huang , William M Katz , Stuart Berg , Louis Scheffer

The promise of extracting connectomes and performing useful analysis on large electron microscopy (EM) datasets has been an elusive dream for many years. Tracing in even the smallest portions of neuropil requires copious human annotation,…

Reconstructing a synaptic wiring diagram, or connectome, from electron microscopy (EM) images of brain tissue currently requires many hours of manual annotation or proofreading (Kasthuri and Lichtman, 2010; Lichtman and Sanes, 2008; Seung,…

Electron microscopy (EM) enables the reconstruction of neural circuits at the level of individual synapses, which has been transformative for scientific discoveries. However, due to the complex morphology, an accurate reconstruction of…

The promise of large-scale, high-resolution datasets from Electron Microscopy (EM) and X-ray Microtomography (XRM) lies in their ability to reveal neural structures and synaptic connectivity, which is critical for understanding the brain.…

3D image segmentation is one of the most important and ubiquitous problems in medical image processing. It provides detailed quantitative analysis for accurate disease diagnosis, abnormal detection, and classification. Currently deep…

Computer Vision and Pattern Recognition · Computer Science 2019-06-19 Zhenxi Zhang , Jie Li , Zhusi Zhong , Zhicheng Jiao , Xinbo Gao

The Pancreatic beta cell is an important target in diabetes research. For scalable modeling of beta cell ultrastructure, we investigate automatic segmentation of whole cell imaging data acquired through soft X-ray tomography. During the…

Quantitative Methods · Quantitative Biology 2020-04-22 John Paul Francis , Hongzhi Wang , Kate White , Tanveer Syeda-Mahmood , Raymond Stevens

The wiring and connectivity of neurons form a structural basis for the function of the nervous system. Advances in volume electron microscopy (EM) and image segmentation have enabled mapping of circuit diagrams (connectomics) within local…

Computer Vision and Pattern Recognition · Computer Science 2023-02-28 Tri Nguyen , Mukul Narwani , Mark Larson , Yicong Li , Shuhan Xie , Hanspeter Pfister , Donglai Wei , Nir Shavit , Lu Mi , Alexandra Pacureanu , Wei-Chung Lee , Aaron T. Kuan

Reconstruction of neuroanatomy is a fundamental problem in neuroscience. Stochastic expression of colors in individual cells is a promising tool, although its use in the nervous system has been limited due to various sources of variability…

Neurons and Cognition · Quantitative Biology 2017-01-24 Uygar Sümbül , Douglas Roussien , Fei Chen , Nicholas Barry , Edward S. Boyden , Dawen Cai , John P. Cunningham , Liam Paninski

Intra-operative ultrasound is an increasingly important imaging modality in neurosurgery. However, manual interaction with imaging data during the procedures, for example to select landmarks or perform segmentation, is difficult and can be…

Computer Vision and Pattern Recognition · Computer Science 2019-04-19 Julia Rackerseder , Rüdiger Göbl , Nassir Navab , Christoph Hennersperger

The prospect of neural reconstruction from Electron Microscopy (EM) images has been elucidated by the automatic segmentation algorithms. Although segmentation algorithms eliminate the necessity of tracing the neurons by hand, significant…

Computer Vision and Pattern Recognition · Computer Science 2015-09-29 Toufiq Parag

Existing learning-based methods to automatically trace axons in 3D brain imagery often rely on manually annotated segmentation labels. Labeling is a labor-intensive process and is not scalable to whole-brain analysis, which is needed for…

Image and Video Processing · Electrical Eng. & Systems 2020-04-22 Tzofi Klinghoffer , Peter Morales , Young-Gyun Park , Nicholas Evans , Kwanghun Chung , Laura J. Brattain

Segmentation of brain structures on magnetic resonance imaging (MRI) is a highly relevant neuroimaging topic, as it is a prerequisite for different analyses such as volumetry or shape analysis. Automated segmentation facilitates the study…

Brain MR image segmentation is a key task in neuroimaging studies. It is commonly conducted using standard computational tools, such as FSL, SPM, multi-atlas segmentation etc, which are often registration-based and suffer from expensive…

Image and Video Processing · Electrical Eng. & Systems 2019-08-29 Chengliang Dai , Yuanhan Mo , Elsa Angelini , Yike Guo , Wenjia Bai

Neural circuit reconstruction at single synapse resolution is increasingly recognized as crucially important to decipher the function of biological nervous systems. Volume electron microscopy in serial transmission or scanning mode has been…

Computer Vision and Pattern Recognition · Computer Science 2018-05-09 Larissa Heinrich , Jan Funke , Constantin Pape , Juan Nunez-Iglesias , Stephan Saalfeld

Extracting a connectome from an electron microscopy (EM) data set requires identification of neurons and determination of synapses between neurons. As manual extraction of this information is very time-consuming, there has been extensive…

Computer Vision and Pattern Recognition · Computer Science 2016-04-12 Gary B. Huang , Louis K. Scheffer , Stephen M. Plaza

Medical image annotation is a major hurdle for developing precise and robust machine learning models. Annotation is expensive, time-consuming, and often requires expert knowledge, particularly in the medical field. Here, we suggest using…

Computer Vision and Pattern Recognition · Computer Science 2020-09-28 Holger R Roth , Dong Yang , Ziyue Xu , Xiaosong Wang , Daguang Xu

Reconstructing the intricate local morphology of neurons and their long-range projecting axons can address many connectivity related questions in neuroscience. The main bottleneck in connectomics pipelines is correcting topological errors,…

Computer Vision and Pattern Recognition · Computer Science 2025-03-05 Anna Grim , Jayaram Chandrashekar , Uygar Sumbul
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