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A laser Doppler imaging scheme combined to an upright microscope is proposed. Quantitative Doppler imaging in both velocity norm and direction, as well as amplitude contrast of either zebrafish flesh or vasculature is demonstrated.

Optics · Physics 2015-01-08 Nicolas Verrier , Daniel Alexandre , Pascal Picart , Michel Gross

We report on local superficial blood flow monitoring in biological tissue from laser Doppler holographic imaging. In time averaging recording conditions, holography acts as a narrowband bandpass filter, which, combined with a frequency…

Laser Doppler holography (LDH) is a full-field blood flow imaging technique able to reveal human retinal and choroidal blood flow with high temporal resolution. We here report on using LDH in the anterior segment of the eye without making…

Laser Doppler holography was introduced as a full-field imaging technique to measure blood flow in the retina and choroid with an as yet unrivaled temporal resolution. We here investigate separating the different contributions to the power…

Medical Physics · Physics 2021-06-02 Léo Puyo , Michel Paques , Mathias Fink , José-Alain Sahel , Michael Atlan

We report on wide-field imaging of pulsatile motion induced by blood flow using heterodyne holographic interferometry on the thumb of a healthy volunteer, in real-time. Optical Doppler images were measured with green laser light by a…

We report a pilot study with a wide-field laser Doppler detection scheme used to perform laser Doppler anemometry and imaging of particle seeded microflow. The optical field carrying the local scatterers (particles) dynamic state, as a…

Optics · Physics 2013-12-24 Michel Gross , Michael Atlan , Jacques Leng

Laser Doppler holography (LDH) is an interferometric blood flow imaging technique based on full-field measurements of the Doppler spectrum. LDH has so far been demonstrated in the retina with ultrafast cameras, typically at 75 kHz. We show…

Image and Video Processing · Electrical Eng. & Systems 2020-08-26 Leo Puyo , Michel Paques , Michael Atlan

Laser Doppler holography (LDH) is a full-field imaging technique that was recently used in the human eye to reveal blood flow contrasts in the retinal and choroidal vasculature non-invasively, and with high temporal resolution. We here…

Medical Physics · Physics 2020-04-01 L. Puyo , M. Paques , M. Fink , J-A Sahel , M. Atlan

Laser Doppler holography is a planar blood flow imaging technique recently introduced in ophthalmology to image human retinal and choroidal blood flow non-invasively. Here we present a digital method based on the Doppler spectrum asymmetry…

Medical Physics · Physics 2021-08-10 Léo Puyo , Michel Paques , Michael Atlan

The eye offers a unique opportunity for non-invasive exploration of cardiovascular diseases. Optical angiography in the retina requires sensitive measurements, which hinders conventional full-field laser Doppler imaging schemes. To overcome…

Medical Physics · Physics 2018-09-18 L. Puyo , M. Paques , M. Fink , J. -A. Sahel , M. Atlan

The choroid is a highly vascularized tissues supplying the retinal pigment epithelium and photoreceptors. Its implication in retinal diseases is gaining increasing interest. However, investigating the anatomy and flow of the choroid remains…

Medical Physics · Physics 2021-06-02 Léo Puyo , Michel Paques , Mathias Fink , José-Alain Sahel , Michael Atlan

We report a demonstration of laser Doppler holography at a sustained acquisition rate of 250 Hz on a 1 Megapixel complementary metal-oxide-semiconductor (CMOS) sensor array and image display at 10 Hz frame rate. The holograms are optically…

Optics · Physics 2015-06-12 Benjamin Samson , Michael Atlan

A comprehensive assessment of retinal health demands reliable and precise methods to measure localized blood perfusion. Despite considerable advancements in imaging techniques, such as indocyanine green and fluorescein angiography, along…

Medical Physics · Physics 2024-03-15 Michael Atlan

Examination of the umbilical artery with Doppler ultrasonography is performed to investigate blood supply to the fetus through the umbilical cord, which is vital for the monitoring of fetal health. Such examination involves several steps…

Doppler holography is an emerging retinal imaging technique that captures the dynamic behavior of blood flow with high temporal resolution, enabling quantitative assessment of retinal hemodynamics. This requires accurate segmentation of…

Computer Vision and Pattern Recognition · Computer Science 2026-02-20 Marius Dubosc , Yann Fischer , Zacharie Auray , Nicolas Boutry , Edwin Carlinet , Michael Atlan , Thierry Geraud

Laser Doppler holography (LDH) is a full-field interferometric imaging technique recently applied in ophthalmology to measure blood flow, a parameter of high clinical interest. From the temporal fluctuations of digital holograms acquired at…

Image and Video Processing · Electrical Eng. & Systems 2020-05-13 Leo Puyo , Michel Paques , Michael Atlan

We report on wide-field imaging of pulsatile microvascular blood flow in the exposed cerebral cortex of a mouse by holographic interferometry. We recorded interferograms of laser light backscattered by the tissue, beating against an…

Medical Physics · Physics 2015-10-08 L. Puyo , I. Ferezou , A. Rancillac , M. Simonutti , M. Paques , J. A. Sahel , M. Fink , M. Atlan

We present a cost-effective, simple and fast digital holographic microscopy method based upon Rayleigh-Sommerfeld back propagation for identification of the geometrical shape of a cell. The method was tested using synthetic hologram images…

Optics · Physics 2015-10-28 Johan Zakrisson , Staffan Schedin , Magnus Andersson

Intravital microscopy is an essential tool that reveals behaviours of live cells under conditions close to natural physiological states. So far, although various approaches for imaging cells in vivo have been proposed, most require the use…

Biological Physics · Physics 2016-07-04 Kyoohyun Kim , Kibaek Choe , Inwon Park , Pilhan Kim , YongKeun Park

Doppler tomography is a method to compute the emissivity distribution within the co-rotating frames of binary stars from observations of their emission line profiles at multiple orbital phases. A key assumption of the method as it is…

Solar and Stellar Astrophysics · Physics 2021-12-08 T. R. Marsh
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