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Phosphorylation, the enzyme-mediated addition of a phosphate group to a molecule, is a ubiquitous chemical mechanism in biology. Multisite phosphorylation, the addition of phosphate groups to multiple sites of a single molecule, may be…

Molecular Networks · Quantitative Biology 2017-06-06 Mitchell Eithun , Anne Shiu

Multisite phosphorylation networks are encountered in many intracellular processes like signal transduction, cell-cycle control or nuclear signal integration. In this contribution networks describing the phosphorylation and…

Molecular Networks · Quantitative Biology 2013-07-03 Katharina Holstein , Dietrich Flockerzi , Carsten Conradi

Multisite protein phosphorylation plays a pivotal role in regulating cellular signaling and decision-making processes. In this study, we focus on the mathematical underpinnings and informational aspects of sequential, distributive…

Molecular Networks · Quantitative Biology 2025-07-22 Iman Tavassoly , Adel Mehrpooya , Parsa Mirlohi , Zahra Abbaspourasadollah

Multistationarity in biological systems is a mechanism of cellular decision making. In particular, signaling pathways regulated by protein phosphorylation display features that facilitate a variety of responses to different biological…

Subcellular Processes · Quantitative Biology 2011-07-05 Elisenda Feliu , Carsten Wiuf

Multisite protein phosphorylation plays a prominent role in intracellular processes like signal transduction, cell-cycle control and nuclear signal integration. Many proteins are phosphorylated in a sequential and distributive way at more…

Molecular Networks · Quantitative Biology 2014-04-24 Dietrich Flockerzi , Katharina Holstein , Carsten Conradi

The multisite phosphorylation-dephosphorylation cycle is a motif repeatedly used in cell signaling. This motif itself can generate a variety of dynamic behaviors like bistability and ultrasensitivity without direct positive feedbacks. In…

Quantitative Methods · Quantitative Biology 2011-11-09 Liming Wang , Eduardo D. Sontag

Multistationarity, underlies biochemical switching and cellular decision-making. We study how multistationarity in the sequential n-site phosphorylation-dephosphorylation cycle is affected when only some species are open, meaning allowed to…

Dynamical Systems · Mathematics 2025-11-14 Praneet Nandan , Beatriz Pascual-Escudero , Diego Rojas La Luz

The dual phosphorylation network provides an essential component of intracellular signaling, affecting the expression of phenotypes and cell metabolism. For particular choices of kinetic parameters, this system exhibits multistationarity, a…

Optimization and Control · Mathematics 2024-09-25 May Cai , Matthias Himmelmann , Birte Ostermann

Ordered distributive double phosphorylation is a recurrent motif in intracellular signaling and control. It is either sequential (where the site phosphorylated last is dephosphorylated first) or cyclic (where the site phosphorylated first…

Molecular Networks · Quantitative Biology 2024-02-09 Carsten Conradi , Maya Mincheva

Oscillations play a major role in a number of biological systems, from predator-prey models of ecology to circadian clocks. In this paper we focus on the question of whether oscillations exist within dual-site phosphorylation systems.…

Molecular Networks · Quantitative Biology 2020-08-03 H. Tung

Multistationarity in molecular systems underlies switch-like responses in cellular decision making. Determining whether and when a system displays multistationarity is in general a difficult problem. In this work we completely determine the…

Molecular Networks · Quantitative Biology 2020-04-15 E. Feliu , N. Kaihnsa , T. de Wolff , O. Yürük

The multiple futile cycle is a phosphorylation system in which a molecular substrate might be phosphorylated sequentially n times by means of an enzymatic mechanism. The system has been studied mathematically using reaction network theory…

Molecular Networks · Quantitative Biology 2020-10-28 Elisenda Feliu , Alan D. Rendall , Carsten Wiuf

We introduce a general framework for biological systems, called MESSI systems, that describe Modifications of type Enzyme-Substrate or Swap with Intermediates, and we prove general results based on the network structure. Many…

Molecular Networks · Quantitative Biology 2018-05-08 Mercedes Pérez Millán , Alicia Dickenstein

This work addresses whether a reaction network, taken with mass-action kinetics, is multistationary, that is, admits more than one positive steady state in some stoichiometric compatibility class. We build on previous work on the effect…

Molecular Networks · Quantitative Biology 2019-08-27 AmirHosein Sadeghimanesh , Elisenda Feliu

Polynomial dynamical systems are widely used to model and study real phenomena. In biochemistry, they are the preferred choice for modelling the concentration of chemical species in reaction networks with mass-action kinetics. These systems…

Algebraic Geometry · Mathematics 2014-12-30 Elisenda Feliu

Background: Using a statistical physics approach, we study the stochastic switching behavior of a model circuit of multisite phosphorylation and dephosphorylation with feedback. The circuit consists of a kinase and phosphatase acting on…

Molecular Networks · Quantitative Biology 2007-10-02 Supriya Krishnamurthy , Eric Smith , David Krakauer , Walter Fontana

The theory of monotone dynamical systems has been found very useful in the modeling of some gene, protein, and signaling networks. In monotone systems, every net feedback loop is positive. On the other hand, negative feedback loops are…

Dynamical Systems · Mathematics 2009-11-13 Liming Wang , Eduardo Sontag

The utilization of multiple phosphorylation sites in regulating a biological response is ubiquitous in cell signaling. If each site contributes an additional, equivalent binding site, then one consequence of an increase in the number of…

Subcellular Processes · Quantitative Biology 2009-11-13 Jason W. Locasale

We apply tools from real algebraic geometry to the problem of multistationarity of chemical reaction networks. A particular focus is on the case of reaction networks whose steady states admit a monomial parametrization. For such systems we…

Molecular Networks · Quantitative Biology 2019-08-14 Carsten Conradi , Alexandru Iosif , Thomas Kahle

Protein phosphorylation cycles are important mechanisms of the post translational modification of a protein and as such an integral part of intracellular signaling and control. We consider the sequential phosphorylation and…

Molecular Networks · Quantitative Biology 2019-11-06 Carsten Conradi , Elisenda Feliu , Maya Mincheva
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