Related papers: Total 3D imaging of phase objects using defocusing…
In this paper the bright-field defocusing microscopy (DM) technique is presented. DM is able to obtain quantitative information of each plane/surface of pure phase objects, as live unlabeled cells, and its application to red blood cells…
We combine confocal imaging, microfluidics and image analysis to record 3D-images of cells in flow. This enables us to recover the full 3D representation of several hundred living cells per minute. Whereas 3D confocal imaging has thus far…
Integrating optical diffraction tomography with imaging flow cytometry enables label-free quantifications of the three-dimensional (3D) morphology and hemoglobin content of red blood cells (RBCs) in their natural form. Self-rotation of RBCs…
Current biological and medical research is aimed at obtaining a detailed spatiotemporal map of a live cell's interior to describe and predict cell's physiological state. We present here an algorithm for complete 3-D modelling of cellular…
We investigate the problem of 3D complex field reconstruction corresponding to unstained red blood cells (RBCs) with a single defocused off-axis digital hologram. We employ recently introduced mean gradient descent (MGD) optimization…
A robust method and strategy for efficient full field-ofview and depth separation optical imaging through scattering media regardless of the three-dimensional (3D) optical memory effect are proposed. In this method, the problem of imaging…
A simple method of constructing the 3D surface of non-transparent micro-objects by extending the depth-of-field on the whole attainable surface is presented. The series of images of a sample are recorded by the sequential movement of the…
This paper presents a novel approach to reconstruct complete 3D deformable models over time by a single depth camera. These are the steps employed for deforming objects from single depth camera. The partial surfaces reconstructed from…
Current in vivo microscopy allows us detailed spatiotemporal imaging (3D+t) of complete organisms and offers insights into their development on the cellular level. Even though the imaging speed and quality is steadily improving,…
Using optical speckle scanning microscopy [1], we demonstrate that clear images of multiple cells can be obtained through biological scattering tissue, with subcellular resolution and good image quality, as long as the size of the imaging…
Optical diffraction tomography (ODT) has emerged as a powerful label-free three-dimensional (3D) bioimaging techniques for observing living cells and thin tissue layers. We report a new reflection-mode ODT (rODT) method for imaging thick…
Analysis of three-dimensional biological samples is critical to understanding tissue function and the mechanisms of disease. Many chronic conditions, like neurodegenerative diseases and cancers, correlate with complex tissue changes that…
The conversion of phase variations in an optical wavefield into intensity information is of fundamental importance for optical imaging technology including microscopy of biological cells. While conventional approaches to phase-imaging…
We propose an approach for 3D reconstruction and segmentation of a single object placed on a flat surface from an input video. Our approach is to perform dense depth map estimation for multiple views using a proposed objective function that…
Biological cell imaging has become one of the most crucial research interests due to its wide-ranging applications in biomedical and microbiology studies. However, three-dimensional (3D) imaging of biological cells remains critically…
We present a novel method for 3D surface reconstruction from multiple images where only a part of the object of interest is captured. Our approach builds on two recent developments: surface reconstruction using neural radiance fields for…
Fluorescence microscopy (FM) imaging is a fundamental technique for observing live cell division, one of the most essential processes in the cycle of life and death. Observing 3D live cells requires scanning through the cell volume while…
Modeling of the red blood cell (RBC) shape is an integral part of the experimental and computer simulation investigations of light scattering by these cells for fundamental studies as well as diagnostic applications in techniques like…
A detailed analysis of ptychography for 3D phase reconstructions of thick specimens is performed. We introduce multi-focus ptychography, which incorporates a 4D-STEM defocus series to enhance the quality of 3D reconstructions along the beam…
Deconvolution phase microscopy enables high-contrast visualization of transparent samples through reconstructions of their transmitted phases or refractive indexes. Herein, we propose a method to extend 2D deconvolution phase microscopy to…