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Sequence alignment data is often ordered by coordinate (id of the reference sequence plus position on the sequence where the fragment was mapped) when stored in BAM files, as this simplifies the extraction of variants between the mapped…

Genomics · Quantitative Biology 2014-06-24 German Tischler , Steven Leonard

This paper introduces a high-throughput software tool framework called {\it sam2bam} that enables users to significantly speedup pre-processing for next-generation sequencing data. The sam2bam is especially efficient on single-node…

Genomics · Quantitative Biology 2017-02-08 Takeshi Ogasawara , Yinhe Cheng , Tzy-Hwa Kathy Tzeng

We here present SIMLR (Single-cell Interpretation via Multi-kernel LeaRning), an open-source tool that implements a novel framework to learn a sample-to-sample similarity measure from expression data observed for heterogenous samples. SIMLR…

Genomics · Quantitative Biology 2018-01-22 Bo Wang , Daniele Ramazzotti , Luca De Sano , Junjie Zhu , Emma Pierson , Serafim Batzoglou

Duplicate marking is a critical preprocessing step in gene sequence analysis to flag redundant reads arising from polymerase chain reaction(PCR) amplification and sequencing artifacts. Although Picard MarkDuplicates is widely recognized as…

Genomics · Quantitative Biology 2025-05-12 Zhonghai Zhang , Yewen Li , Ke Meng , Chunming Zhang , Guangming Tan

Motivation: Modern genomics laboratories generate massive volumes of sequencing data, often resulting in significant storage costs. Genomics storage consists of duplicate files, temporary processing files, and redundant intermediate data.…

Genomics · Quantitative Biology 2025-11-27 Margot Celerie , Andrew Oldfield , William Ritchie

DNA sequencing, especially of microbial genomes and metagenomes, has been at the core of recent research advances in large-scale comparative genomics. The data deluge has resulted in exponential growth in genomic datasets over the past…

Motivation: Computational methods are essential to extract actionable information from raw sequencing data, and to thus fulfill the promise of next-generation sequencing technology. Unfortunately, computational tools developed to call…

The exponential growth of DNA sequencing data has outpaced traditional heuristic-based methods, which struggle to scale effectively. Efficient computational approaches are urgently needed to support large-scale similarity search, a…

Advancing research in fields such as Simultaneous Localization and Mapping (SLAM) and autonomous navigation critically depends on the availability of reliable and reproducible multimodal datasets. While several influential datasets have…

Transcript enumeration methods such as SAGE, MPSS, and sequencing-by-synthesis EST ``digital northern'', are important high-throughput techniques for digital gene expression measurement. As other counting or voting processes, these…

Quantitative Methods · Quantitative Biology 2013-10-29 Ricardo ZN Vêncio , Leonardo Varuzza , Carlos AB Pereira , Helena Brentani , Ilya Shmulevich

To increase performance and efficiency, systems use FPGAs as reconfigurable accelerators. A key challenge in designing these systems is partitioning computation between processors and an FPGA. An appropriate division of labor may be…

Hardware Architecture · Computer Science 2021-07-21 Endri Bezati , Mahyar Emami , Jörn Janneck , James Larus

Transcriptome assembly from RNA-Seq reads is an active area of bioinformatics research. The ever-declining cost and the increasing depth of RNA-Seq have provided unprecedented opportunities to better identify expressed transcripts. However,…

Computational Engineering, Finance, and Science · Computer Science 2013-06-07 Tin Chi Nguyen , Zhiyu Zhao , Dongxiao Zhu

The high-throughput short-reads RNA-seq protocols often produce paired-end reads, with the middle portion of the fragments being unsequenced. We explore if the full-length fragments can be computationally reconstructed from the sequenced…

Genomics · Quantitative Biology 2023-10-06 Xiang Li , Mingfu Shao

A genome read data set can be quickly and efficiently remapped from one reference to another similar reference (e.g., between two reference versions or two similar species) using a variety of tools, e.g., the commonly-used CrossMap tool.…

Genomics · Quantitative Biology 2023-11-21 Jeremie S. Kim , Can Firtina , Meryem Banu Cavlak , Damla Senol Cali , Can Alkan , Onur Mutlu

We present a parallel algorithm and scalable implementation for genome analysis, specifically the problem of finding overlaps and alignments for data from "third generation" long read sequencers. While long sequences of DNA offer enormous…

Distributed, Parallel, and Cluster Computing · Computer Science 2020-01-29 Marquita Ellis , Giulia Guidi , Aydın Buluç , Leonid Oliker , Katherine Yelick

Background: With the rapid growth of massively parallel sequencing technologies, still more laboratories are utilizing sequenced DNA fragments for genomic analyses. Interpretation of sequencing data is, however, strongly dependent on…

It is a challenging task to train large DNN models on sophisticated GPU platforms with diversified interconnect capabilities. Recently, pipelined training has been proposed as an effective approach for improving device utilization. However,…

Distributed, Parallel, and Cluster Computing · Computer Science 2020-07-03 Shiqing Fan , Yi Rong , Chen Meng , Zongyan Cao , Siyu Wang , Zhen Zheng , Chuan Wu , Guoping Long , Jun Yang , Lixue Xia , Lansong Diao , Xiaoyong Liu , Wei Lin

Currently, third-generation sequencing techniques, which allow to obtain much longer DNA reads compared to the next-generation sequencing technologies, are becoming more and more popular. There are many possibilities to combine data from…

Genomics · Quantitative Biology 2019-05-23 Wiktor Kuśmirek , Wiktor Franus , Robert Nowak

Input pipelines, which ingest and transform input data, are an essential part of training Machine Learning (ML) models. However, it is challenging to implement efficient input pipelines, as it requires reasoning about parallelism,…

Machine Learning · Computer Science 2022-03-22 Michael Kuchnik , Ana Klimovic , Jiri Simsa , Virginia Smith , George Amvrosiadis

Raw nanopore signal analysis is a common approach in genomics to provide fast and resource-efficient analysis without translating the signals to bases (i.e., without basecalling). However, existing solutions cannot interpret raw signals…

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