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Cell heterogeneity and the inherent complexity due to the interplay of multiple molecular processes within the cell pose difficult challenges for current single-cell biology. We introduce an approach that identifies a disease phenotype from…

Many pressing medical challenges - such as diagnosing disease, enhancing directed stem cell differentiation, and classifying cancers - have long been hindered by limitations in our ability to quantify proteins in single cells.…

Genomics · Quantitative Biology 2018-08-06 Harrison Specht , Nikolai Slavov

The application of single-cell molecular profiling coupled with spatial technologies has enabled charting cellular heterogeneity in reference tissues and in disease. This new wave of molecular data has highlighted the expected diversity of…

Tissues and Organs · Quantitative Biology 2024-03-12 Ricardo Omar Ramirez Flores , Philipp Sven Lars Schäfer , Leonie Küchenhoff , Julio Saez-Rodriguez

New technologies have enabled the investigation of biology and human health at an unprecedented scale and in multiple dimensions. These dimensions include a myriad of properties describing genome, epigenome, transcriptome, microbiome,…

Quantitative Methods · Quantitative Biology 2018-10-22 Marinka Zitnik , Francis Nguyen , Bo Wang , Jure Leskovec , Anna Goldenberg , Michael M. Hoffman

Multimodal single-cell technologies enable the simultaneous collection of diverse data types from individual cells, enhancing our understanding of cellular states. However, the integration of these datatypes and modeling the…

Machine Learning · Computer Science 2023-11-22 Bhavya Mehta , Nirmit Deliwala , Madhav Chandane

Single-cell technologies have revolutionized biomedical research by enabling scalable measurement of the genome, transcriptome, and proteome of multiple systems at single-cell resolution. Now widely applied to cancer models, these assays…

Genomics · Quantitative Biology 2020-05-05 Allen W Zhang , Kieran R Campbell

Understanding the mechanisms of interactions within cells, tissues, and organisms is crucial to driving developments across biology and medicine. Mathematical modeling is an essential tool for simulating biological systems and revealing…

Molecular Networks · Quantitative Biology 2024-08-13 Lingxia Qiao , Ali Khalilimeybodi , Nathaniel J Linden-Santangeli , Padmini Rangamani

Biological functions stem from coordinated interactions among proteins, nucleic acids and small molecules. Mass spectrometry technologies for reliable, high throughput single-cell proteomics will add a new modality to genomics and enable…

Genomics · Quantitative Biology 2023-11-07 Andrew Leduc , Hannah Harens , Nikolai Slavov

Recently developed technologies to generate single-cell genomic data have made a revolutionary impact in the field of biology. Multi-omics assays offer even greater opportunities to understand cellular states and biological processes.…

Genomics · Quantitative Biology 2022-01-19 Stefan Stanojevic , Yijun Li , Lana X. Garmire

Single-cell proteomics (SCP) is transforming our understanding of biological complexity by shifting from bulk proteomics, where signals are averaged over thousands of cells, to the proteome analysis of individual cells. This granular…

Quantitative Methods · Quantitative Biology 2025-04-01 Amanda Momenzadeh , Jesse G. Meyer

Cell competition is a quality control mechanism in tissues that results in the elimination of less fit cells. Over the past decade, the phenomenon of cell competition has been identified in many physiological and pathological contexts,…

Tissues and Organs · Quantitative Biology 2019-03-26 Daniel Gradeci , Anna Bove , Guillaume Charras , Alan R. Lowe , Shiladitya Banerjee

The development of single-cell and spatial transcriptomics has revolutionized our capacity to investigate cellular properties, functions, and interactions in both cellular and spatial contexts. However, the analysis of single-cell and…

Genomics · Quantitative Biology 2024-12-09 Shuang Ge , Shuqing Sun , Huan Xu , Qiang Cheng , Zhixiang Ren

Cellular signaling is essential in information processing and decision making. Therefore, a variety of experimental approaches have been developed to study signaling on bulk and single-cell level. Single-cell measurements of signaling…

Quantitative Methods · Quantitative Biology 2019-04-18 Carolin Loos , Jan Hasenauer

A rigorous understanding of how multicellular behaviors arise from the actions of single cells requires quantitative frameworks that bridge the gap between genetic circuits, the arrangement of cells in space, and population-level behaviors.…

Molecular Networks · Quantitative Biology 2016-02-18 Théo Maire , Hyun Youk

Single-molecule biophysics has transformed our understanding of the fundamental molecular processes involved in living biological systems, but also of the fascinating physics of life. Far more exotic than a collection of exemplars of soft…

Biological Physics · Physics 2017-09-08 Helen Miller , Zhaokun Zhou , Jack Shepherd , Adam J. M. Wollman , Mark C. Leake

Cellular heterogeneity is an immanent property of biological systems that covers very different aspects of life ranging from genetic diversity to cell-to-cell variability driven by stochastic molecular interactions, and noise induced cell…

Quantitative Methods · Quantitative Biology 2021-09-15 Niko Komin , Alexander Skupin

Whole-cell computational models aim to predict cellular phenotypes from genotype by representing the entire genome, the structure and concentration of each molecular species, each molecular interaction, and the extracellular environment.…

Quantitative Methods · Quantitative Biology 2019-09-05 Arthur P. Goldberg , Balázs Szigeti , Yin Hoon Chew , John A. P. Sekar , Yosef D. Roth , Jonathan R. Karr

Recent advances in single cell sequencing and multi-omics techniques have significantly improved our understanding of biological phenomena and our capacity to model them. Despite combined capture of data modalities showing similar progress,…

Molecular Networks · Quantitative Biology 2025-03-13 Marcello Barylli , Joyaditya Saha , Tineke E. Buffart , Jan Koster , Kristiaan J. Lenos , Louis Vermeulen , Vivek M. Sheraton

Single-cell analysis is currently one of the most high-resolution techniques to study biology. The large complex datasets that have been generated have spurred numerous developments in computational biology, in particular the use of…

Genomics · Quantitative Biology 2023-04-27 Ionut Sebastian Mihai , Sarang Chafle , Johan Henriksson

Over the last decade, proteomic analysis of single cells by mass spectrometry transitioned from an uncertain possibility to a set of robust and rapidly advancing technologies supporting the accurate quantification of thousands of proteins.…

Quantitative Methods · Quantitative Biology 2026-04-14 Nikolai Slavov
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