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Enzyme-catalysed reactions involve two distinct timescales. There is a short timescale on which enzymes bind to substrate molecules to produce bound complexes, and a comparatively long timescale on which the complex is transformed into a…

Quantitative Methods · Quantitative Biology 2023-10-06 Taylor Kearney , Mark B. Flegg

Scaling analysis exploiting timescale separation has been one of the most important techniques in the quantitative analysis of nonlinear dynamical systems in mathematical and theoretical biology. In the case of enzyme catalyzed reactions,…

Quantitative Methods · Quantitative Biology 2023-03-21 Justin Eilertsen , Wylie Stroberg , Santiago Schnell

Recent experimental results have shown that active enzymes can diffuse faster when they are in the presence of their substrates. Fluorescence correlation spectroscopy (FCS), which relies on analyzing the fluctuations in fluorescence…

Subcellular Processes · Quantitative Biology 2019-09-25 Mengqi Xu , Lyanne Valdez , Aysuman Sen , Jennifer L. Ross

Cells generally convert nutrient resources to useful products via energy transduction. Accordingly, the thermodynamic efficiency of this conversion process is one of the most essential characteristics of living organisms. However, although…

Biological Physics · Physics 2016-05-04 Yusuke Himeoka , Kunihiko Kaneko

Biochemical systems are inherently stochastic, particularly those with small-molecule populations. The spatial distribution of molecules plays a critical role and requires the inclusion of spatial coordinates in their analysis. Stochastic…

Molecular Networks · Quantitative Biology 2025-05-15 Manuel Eduardo Hernández-García , Eduardo Moreno-Barbosa , Jorge Velázquez-Castro

Intracellular transport is vital for the proper functioning and survival of a cell. Cargo (proteins, vesicles, organelles, etc.) is transferred from its place of creation to its target locations via molecular motor assisted transport along…

Biological Physics · Physics 2016-11-24 Anne E. Hafner , Heiko Rieger

We investigate a model for spatial epidemics explicitly taking into account bi-directional movements between base and destination locations on individual mobility networks. We provide a systematic analysis of generic dynamical features of…

Physics and Society · Physics 2012-03-07 Vitaly Belik , Theo Geisel , Dirk Brockmann

Intracellular transport processes are essential to the healthy development of many organisms as well as more generally to healthy cellular function. The complex dynamics and interactions between protein molecules and filaments on different…

Dynamical Systems · Mathematics 2022-07-27 Maria-Veronica Ciocanel

The formation of condensates is now considered as a major organization principle of eukaryotic cells. Several studies have recently shown that the properties of these condensates are affected by enzymatic reactions. We propose here a simple…

Soft Condensed Matter · Physics 2024-11-19 Jacques Fries , Javier Diaz , Marie Jardat , Ignacio Pagonabarraga , Pierre Illien , Vincent Dahirel

Multienzyme cascaded reactions are widely utilized because they can generate value-added biomaterials and biodevices from simple raw materials. However, how to promote the catalytic efficiency and synergistic effect of the multienzyme…

Biological Physics · Physics 2021-01-27 Zhexu Xi

There is a crescent use of enzymes in multiple industries and sciences, ranging from materials and fuel synthesis to pharmaceutical and food production. Their applicability in this variety of fields depends not only on their biochemical…

Molecular Networks · Quantitative Biology 2022-05-12 Renan dos Reis , Luciano da F. Costa

Strong experimental and theoretical evidence shows that transcription factors and other specific DNA-binding proteins find their sites using a two-mode search: alternating between 3D diffusion through the cell and 1D sliding along the DNA.…

Biomolecules · Quantitative Biology 2008-06-11 Zeba Wunderlich , Leonid A. Mirny

Experiments show that the movement of eukaryotic cells is regulated by a process of phase separation of two competing enzymes on the cell membrane, that effectively amplifies shallow external gradients of chemical attractant. Notably, the…

Statistical Mechanics · Physics 2008-12-19 Teresa Ferraro , Antonio de Candia , Andrea Gamba , Antonio Coniglio

We investigated the energy transfer from active enzymes to their surroundings in crowded environments by measuring the diffusion of passive microscopic tracers in active solutions of ficoll and glycerol. Despite observing lower rates of…

In many sensory systems, transmembrane receptors are spatially organized in large clusters. Such arrangement may facilitate signal amplification and the integration of multiple stimuli. However, this organization likely also affects the…

Molecular Networks · Quantitative Biology 2014-03-05 William Pontius , Michael W. Sneddon , Thierry Emonet

Fluctuations in the abundance of molecules in the living cell may affect its growth and well being. For regulatory molecules (e.g., signaling proteins or transcription factors), fluctuations in their expression can affect the levels of…

Molecular Networks · Quantitative Biology 2009-11-13 Erel Levine , Terence Hwa

Spatial heterogeneity is a hallmark of living systems, even at the molecular scale in individual cells. A key example is the partitioning of membrane-bound proteins via lipid domain formation or cytoskeleton-induced corralling. Yet the…

Molecular Networks · Quantitative Biology 2013-11-19 Andrew Mugler , Filipe Tostevin , Pieter Rein ten Wolde

Dynamic cooperativity in monomeric enzymes is characterized in terms of a non-Michaelis-Menten kinetic behaviour. The latter is believed to be associated with mechanisms that include multiple reaction pathways due to enzymatic…

Chemical Physics · Physics 2016-09-21 Ashutosh Kumar , Sambarta Chatterjee , Mintu Nandi , Arti Dua

A protein undergoes conformational dynamics with multiple time scales, which results in fluctuating enzyme activities. Recent studies in single molecule enzymology have observe this "age-old" dynamic disorder phenomenon directly. However,…

Biomolecules · Quantitative Biology 2009-11-10 Zhanghan Wu , Vlad Elgart , Hong Qian , Jianhua Xing

Chemical reactions are usually studied under the assumption that both substrates and catalysts are well mixed (WM) throughout the system. Although this is often applicable to test-tube experimental conditions, it is not realistic in…

Statistical Mechanics · Physics 2023-02-13 Nino Lauber , Ondrej Tichacek , Krishnadev Narayanankutty , Daniele De Martino , Kepa Ruiz-Mirazo