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We consider self-assembly of proteins into a virus capsid by the methods of molecular dynamics. The capsid corresponds either to SPMV or CCMV and is studied with and without the RNA molecule inside. The proteins are flexible and described…

Biomolecules · Quantitative Biology 2018-11-05 Karol Wolek , Marek Cieplak

Recent studies reveal that certain viruses package a portion of their genome in a manner that mirrors the icosahedral symmetry of the protein container, or capsid. Graph theoretical constraints forbid exact realization of icosahedral…

Soft Condensed Matter · Physics 2007-05-23 Joseph Rudnick , Robijn Bruinsma

A series of simulations aimed at elucidating the self-assembly dynamics of spherical virus capsids is described. This little-understood phenomenon is a fascinating example of the complex processes that occur in the simplest of organisms.…

Soft Condensed Matter · Physics 2015-05-19 D. C. Rapaport

We propose a description for the quasi-equilibrium self-assembly of small, single-stranded (ss) RNA viruses whose capsid proteins (CPs) have flexible, positively charged, disordered tails that associate with the negatively charged RNA…

Soft Condensed Matter · Physics 2016-03-23 Robijn F. Bruinsma , Mauricio Comas-Garcia , Rees F. Garmann , Alexander Y. Grosberg

Self-assembly at submicroscopic scales is an important but little understood phenomenon. A prominent example is virus capsid growth, whose underlying behavior can be modeled using simple particles that assemble into polyhedral shells.…

Soft Condensed Matter · Physics 2008-10-28 D. C. Rapaport

I present a review of the theoretical and computational methodologies that have been used to model the assembly of viral capsids. I discuss the capabilities and limitations of approaches ranging from equilibrium continuum theories to…

Biomolecules · Quantitative Biology 2013-01-09 Michael F. Hagan

The kinetics for the assembly of viral proteins into a population of capsids can be measured in vitro with size exclusion chromatography or dynamic light scattering, but extracting mechanistic information from these studies is challenging.…

Biomolecules · Quantitative Biology 2015-05-13 Michael F. Hagan

For many viruses assembly and budding occur simultaneously during virion formation. Understanding the mechanisms underlying this process could promote biomedical efforts to block viral propagation and enable use of capsids in nanomaterials…

Subcellular Processes · Quantitative Biology 2014-03-11 Teresa Ruiz-Herrero , Michael F. Hagan

In order to replicate within their cellular host, many viruses have developed self-assembly strategies for their capsids which are sufficiently robust as to be reconstituted in vitro. Mathematical models for virus self-assembly usually…

Subcellular Processes · Quantitative Biology 2015-02-03 Johanna E. Baschek , Heinrich C. R. Klein , Ulrich S. Schwarz

Understanding the pathways by which viral capsid proteins assemble around their genomes could identify key intermediates as potential drug targets. In this work we use computer simulations to characterize assembly over a wide range of…

Biomolecules · Quantitative Biology 2014-05-15 Jason D Perlmutter , Matthew R Perkett , Michael F Hagan

The coat proteins of many viruses spontaneously form icosahedral capsids around nucleic acids or other polymers. Elucidating the role of the packaged polymer in capsid formation could promote biomedical efforts to block viral replication…

Biomolecules · Quantitative Biology 2015-05-19 Oren M. Elrad , Michael F. Hagan

We model the spontaneous assembly of a capsid (a virus's closed outer shell) from many copies of identical units, using entirely irreversible steps and only information local to the growing edge. Our model is formulated in terms of (i) an…

Biomolecules · Quantitative Biology 2009-11-11 Stephen D. Hicks , C. L. Henley

A big class of viruses self-assemble from a large number of identical capsid proteins with long flexible N-terminal tails and ss RNA. We study the role of the strong Coulomb interaction of positive N-terminal tails with ss RNA in the…

Biomolecules · Quantitative Biology 2009-11-13 Tao Hu , B. I. Shklovskii

Previous self-assembly experiments on a model icosahedral plant virus have shown that, under physiological conditions, capsid proteins initially bind to the genome through an en masse mechanism and form nucleoprotein complexes in a…

Biological Physics · Physics 2020-04-02 Sanaz Panahandeh , Siyu Li , Laurent Marichal , Rafael Leite Rubim , Guillaume Tresset , Roya Zandi

A vital constituent of a virus is its protein shell, called the viral capsid, that encapsulates and hence provides protection for the viral genome. Assembly models are developed for viral capsids built from protein building blocks that can…

Biomolecules · Quantitative Biology 2008-05-15 T. Keef , A. Taormina , R. Twarock

Many spherical viruses encapsulate their genome in protein shells with icosahedral symmetry. This process is spontaneous and driven by electrostatic interactions between positive domains on the virus coat proteins and the negative genome.…

Biological Physics · Physics 2017-08-16 Siyu Li , Gonca Erdemci-Tandogan , Jef Wagner , Paul van der Schoot , Roya Zandi

We study the self-assembly behaviour of patchy particles with `protein-like' interactions that can be considered as a minimal model for the assembly of viral capsids and other shell-like protein complexes. We thoroughly explore the…

Soft Condensed Matter · Physics 2010-01-21 Alex W. Wilber , Jonathan P. K. Doye , Ard A. Louis , Anna C. F. Lewis

The problem of RNA genomes packaged inside spherical viruses is studied. The viral capsid is modeled as a hollowed sphere. The attraction between RNA molecules and the inner viral capsid is assumed to be non-specific and occurs at the inner…

Soft Condensed Matter · Physics 2009-11-13 Se Il Lee , T. T. Nguyen

Under many in vitro conditions, some small viruses spontaneously encapsidate a single stranded (ss) RNA into a protein shell called the capsid. While viral RNAs are found to be compact and highly branched because of long distance…

Biological Physics · Physics 2018-01-17 Siyu Li , Gonca Erdemci-Tandogan , Paul van der Schoot , Roya Zandi

We develop a class of models with which we simulate the assembly of particles into T1 capsid-like objects using Newtonian dynamics. By simulating assembly for many different values of system parameters, we vary the forces that drive…

Biomolecules · Quantitative Biology 2009-11-11 Michael F. Hagan , David Chandler