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Length-regulation of microtubules (MTs) is essential for many cellular processes. Molecular motors like kinesin 8, which move along MTs and also act as depolymerases, are known as key players in MT dynamics. However, the regulatory…

Subcellular Processes · Quantitative Biology 2012-10-18 Anna Melbinger , Louis Reese , Erwin Frey

The cytoskeleton is regulated by a plethora of enzymes that influence the stability and dynamics of cytoskeletal filaments. Molecular motors of the kinesin-8 protein family depolymerise microtubules in a length-dependent manner, and…

Subcellular Processes · Quantitative Biology 2014-10-29 Louis Reese , Anna Melbinger , Erwin Frey

Proteins from the kinesin-8 family promote microtubule (MT) depolymerization, a process thought to be important for the control of microtubule length in living cells. In addition to this MT shortening activity, kinesin 8s are motors that…

Biological Physics · Physics 2015-05-13 L. E. Hough , Anne Schwabe , Matthew A. Glaser , J. Richard McIntosh , M. D. Betterton

The availability of protein is an important factor for the determination of the size of the mitotic spindle. Involved in spindle-size regulation is kinesin-8, a molecular motor and microtubule (MT) depolymerase, which is known to tightly…

Biological Physics · Physics 2018-04-10 Matthias Rank , Aniruddha Mitra , Louis Reese , Stefan Diez , Erwin Frey

Cytoskeletal networks form complex intracellular structures. Here we investigate a minimal model for filament-motor mixtures in which motors act as depolymerases and thereby regulate filament length. Combining agent-based simulations and…

Biological Physics · Physics 2022-12-14 Moritz Striebel , Fridtjof Brauns , Erwin Frey

In many intracellular processes, the length distribution of microtubules is controlled by depolymerizing motor proteins. Experiments have shown that, following non-specific binding to the surface of a microtubule, depolymerizers are…

Biological Physics · Physics 2009-11-13 Bindu S. Govindan , Manoj Gopalakrishnan , Debashish Chowdhury

Assembly and disassembly dynamics of microtubules (MTs) is tightly controlled by MT associated proteins. Here, we investigate how plus-end-directed depolymerases of the kinesin-8 family regulate MT depolymerization dynamics. Employing an…

Biological Physics · Physics 2011-11-15 Louis Reese , Anna Melbinger , Erwin Frey

Motor proteins that specifically interact with the ends of cytoskeletal filaments can induce filament depolymerization. A phenomenological description of this process is presented. We show that under certain conditions motors dynamically…

Subcellular Processes · Quantitative Biology 2009-11-11 Gernot A. Klein , Karsten Kruse , Gianaurelio Cuniberti , Frank Juelicher

The microtubule cytoskeleton is responsible for sustained, long-range intracellular transport of mRNAs, proteins, and organelles in neurons. Neuronal microtubules must be stable enough to ensure reliable transport, but they also undergo…

Dynamical Systems · Mathematics 2024-03-05 Anna C Nelson , Melissa M Rolls , Maria-Veronica Ciocanel , Scott A McKinley

In this work we study a microtubule (MT) model, whose length is regulated by the action of processive kinesin motors. We treat the case of infinite processivity, i.e. particle exchange in the bulk is neglected. The exact results can be…

Statistical Mechanics · Physics 2015-06-25 Chikashi Arita , Alexander Lück , Ludger Santen

Microtubules are protein polymers that form "molecular highways" for long-range transport within living cells. Molecular motors actively step along microtubules to shuttle cellular materials between the nucleus and the cell periphery; this…

Biomolecules · Quantitative Biology 2016-10-06 Winnie H. Liang , Qiaochu Li , K. M. Rifat Faysal , Stephen J. King , Ajay Gopinathan , Jing Xu

The origin of biological motion can be traced back to the function of molecular motor proteins. Cytoplasmic dynein and kinesin transport organelles within our cells moving along a polymeric filament, the microtubule. The motion of the…

Statistical Mechanics · Physics 2015-06-25 Imre Derenyi , Tamas Vicsek

Cells contain elaborate and interconnected networks of protein polymers which make up the cytoskeleton. The cytoskeleton governs the internal positioning and movement of vesicles and organelles, and controls dynamic changes in cell…

Biological Physics · Physics 2017-01-24 Lishibanya Mohapatra , Bruce L. Goode , Predrag Jelenkovic , Rob Phillips , Jane Kondev

Certain regulatory proteins influence the polymerization dynamics of microtubules by inducing catastrophe with a rate that depends on the microtubule length. Using a discrete formulation, here we show that, for a catastrophe rate…

Biological Physics · Physics 2012-08-27 Vandana Yadav , Sutapa Mukherji

Microtubules (MTs) are dynamic protein filaments essential for intracellular organization and transport, particularly in long-lived cells such as neurons. The plus and minus ends of neuronal MTs switch between growth and shrinking phases,…

Biological Physics · Physics 2025-07-11 Anna C. Nelson , Scott A. McKinley , Melissa M. Rolls , Maria-Veronica Ciocanel

Motor-driven cytoskeletal remodeling in cellular systems can often be accompanied by a diffusive-like effect at local scales, but distinguishing the contributions of the ordering process, such as active contraction of a network, from this…

Biological Physics · Physics 2024-08-22 Soichi Hirokawa , Heun Jin Lee , Rachel A Banks , Ana I Duarte , Bibi Najma , Matt Thomson , Rob Phillips

The functioning of machines typically requires a concerted action of their parts. This requirement also holds for molecular motors that drive vital cellular processes and imposes constraints on their conformational changes as well as the…

Biological Physics · Physics 2022-06-14 Zena Hadjivasiliou , Karsten Kruse

Kinesin motors can induce a buckling instability in a microtubule with a fixed minus end. Here we show that by modifying the surface with a protein-repellent functionalization and using clusters of kinesin motors, the microtubule can…

Cells are strongly out-of-equilibrium systems driven by continuous energy supply. They carry out many vital functions requiring active transport of various ingredients and organelles, some being small, others being large. The cytoskeleton,…

Biological Physics · Physics 2017-09-20 Cecile Appert-Rolland , Maximilian Ebbinghaus , Ludger Santen

In cells and in vitro assays the number of motor proteins involved in biological transport processes is far from being unlimited. The cytoskeletal binding sites are in contact with the same finite reservoir of motors (either the cytosol or…

Subcellular Processes · Quantitative Biology 2014-09-26 Luca Ciandrini , I. Neri , Jean-Charles Walter , O. Dauloudet , A. Parmeggiani
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