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Developing rapid methods for pathogen detection and growth monitoring at low cell and analyte concentrations is an important goal, which numerous technologies are working towards solving. Rapid biosensors have already made a dramatic impact…

Traditional microbial detection methods often rely on the overall property of microbial cultures and cannot resolve individual growth event at high spatiotemporal resolution. As a result, they require bacteria to grow to confluence and then…

We present a computational live bacteria detection system that periodically captures coherent microscopy images of bacterial growth inside a 60 mm diameter agar-plate and analyzes these time-lapsed holograms using deep neural networks for…

We develop a mother machine-like microfluidic device specifically designed to track the proliferation of T-cells via live-cell microscopy. Although numerous microfluidic setups have been developed to study cell proliferation at the…

Cell Behavior · Quantitative Biology 2025-09-09 Simone Scalise , Giovanna Peruzzi , Davide Caprini , Giorgio Gosti , Giancarlo Ruocco , Mattia Miotto

A hallmark of bacterial populations cultured in vitro is their homogeneity of growth, where the majority of cells display identical growth rate, cell size and content. Recent insights, however, have revealed that even cells growing in…

Quantitative Methods · Quantitative Biology 2022-06-07 Santosh Pandey , Yunsoo Park , Ankita Ankita , Gregory J. Phillips

Use of commercial growth chambers for study of biological processes involved in biomass growth and production pose certain limitations on the nature of studies that can be performed in them. Optimization of biomass rearing and production…

Quantitative Methods · Quantitative Biology 2020-04-16 Murali Padmanabha , Stefan Streif

A computational model for the simulation of a double band collector-generator experiment is applied to the situation where two electrochemical reactions occur concurrently. It is shown that chronoamperometric measurements can be used to…

Chemical Physics · Physics 2013-10-28 Edward O. Barnes , Grace E. M. Lewis , Sara E. C. Dale , Frank Marken , Richard G. Compton

To understand dynamic developmental processes, living tissues must be imaged frequently and for extended periods of time. Root development is extensively studied at cellular resolution to understand basic mechanisms underlying pattern…

Tissues and Organs · Quantitative Biology 2015-03-13 Giovanni Sena , Zak Frentz , Kenneth D. Birnbaum , Stanislas Leibler

To visualize the topography of thin oxide films during growth, thereby enabling to study its growth behavior quasi real-time, we have designed and integrated an atomic force microscope (AFM) in a pulsed laser deposition (PLD) vacuum setup.…

Materials Science · Physics 2017-12-11 W. A. Wessels , T. R. J. Bollmann , D. Post , G. Koster , G. Rijnders

The Absolute Radiometer for Cosmology, Astrophysics, and Diffuse Emission (ARCADE) is a balloon-borne instrument to measure the temperature of the cosmic microwave background at centimeter wavelengths. ARCADE uses narrow-band cryogenic…

Astrophysics · Physics 2009-11-10 A. Kogut , D. J. Fixsen , S. Levin , M. Limon , P. M. Lubin , P. Mirel , M. Seiffert , E. Wollack

This paper reports on the development of a novel CMOS device employing lead-sensing bacteria to assay lead in drinking water. The objective of the PlomBOX project is to develop a low-cost sensor ({\pounds}10) which can expedite access to…

Microalgae counting is used to measure biomass quantity. Usually, it is performed in a manual way using a Neubauer chamber and expert criterion, with the risk of a high error rate. This paper addresses the methodology for automatic…

Current in vivo microscopy allows us detailed spatiotemporal imaging (3D+t) of complete organisms and offers insights into their development on the cellular level. Even though the imaging speed and quality is steadily improving,…

Image and Video Processing · Electrical Eng. & Systems 2020-01-31 Sourabh Bhide , Ralf Mikut , Maria Leptin , Johannes Stegmaier

Despite numerous advances in the field of tissue engineering and regenerative medicine, monitoring the formation of tissue regeneration and its metabolic variations during culture is still a challenge and mostly limited to bulk volumetric…

Nutrient gradients and limitations play a pivotal role in the life of all microbes, both in their natural habitat as well as in artificial, microfluidic systems. Spatial concentration gradients of nutrients in densely packed cell…

A novel readout architecture that uses multiple non-destructive floating-gate amplifiers to achieve sub-electron readout noise in a thick, fully-depleted silicon detector is presented. This Multi-Amplifier Sensing Charge-Coupled Device…

We present an apparatus optimized for tracking swimming microorganisms in the size range 10-1000 microns, in three dimensions (3D), far from surfaces, and with negligible background convective fluid motion. CCD cameras attached to two long…

Soft Condensed Matter · Physics 2009-01-13 Knut Drescher , Kyriacos C. Leptos , Raymond E. Goldstein

This paper presents AlphaGarden: an autonomous polyculture garden that prunes and irrigates living plants in a 1.5m x 3.0m physical testbed. AlphaGarden uses an overhead camera and sensors to track the plant distribution and soil moisture.…

Dynamic operation of anaerobic digestion plants requires advanced process monitoring and control. Different simplifications of the Anaerobic Digestion Model No. 1 (ADM1) have been proposed recently, which appear promising for model-based…

Systems and Control · Electrical Eng. & Systems 2023-04-06 Simon Hellmann , Arne-Jens Hempel , Stefan Streif , Sören Weinrich

Confocal Raman microscopy offers a particular pathway for monitoring chemical "fingerprints" of intracellular components like the cell membrane, organelles, and nucleus. Nevertheless, conventional Raman acquisitions of fixed or randomly…

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