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Single-cell data integration can provide a comprehensive molecular view of cells, and many algorithms have been developed to remove unwanted technical or biological variations and integrate heterogeneous single-cell datasets. Despite their…

Quantitative Methods · Quantitative Biology 2024-03-04 Rong Ma , Eric D. Sun , David Donoho , James Zou

Automatic test generation aims to save developers time and effort by producing test suites with reasonably high coverage and fault detection. However, the focus of search-based generation tools in maximizing coverage leaves other…

Software Engineering · Computer Science 2025-04-11 Geraldine Galindo-Gutierrez

Single-cell transcriptomics techniques, such as scRNA-seq, attempt to characterize gene expression profiles in each cell of a heterogeneous sample individually. Due to growing amounts of data generated and the increasing complexity of the…

Genomics · Quantitative Biology 2023-05-02 Laura Puente-Santamaría , Luis del Peso

High-throughput RNA sequencing (RNA-seq) is now the standard method to determine differential gene expression. Identifying differentially expressed genes crucially depends on estimates of read count variability. These estimates are…

We introduce a novel framework for individual-level welfare analysis. It builds on a parametric model for continuous demand with a quasilinear utility function, allowing for heterogeneous coefficients and unobserved individual-good-level…

Econometrics · Economics 2024-11-25 Junlong Feng , Sokbae Lee

Modern single-cell flow and mass cytometry technologies measure the expression of several proteins of the individual cells within a blood or tissue sample. Each profiled biological sample is thus represented by a set of hundreds of…

Machine Learning · Computer Science 2022-06-29 Siyuan Shan , Vishal Baskaran , Haidong Yi , Jolene Ranek , Natalie Stanley , Junier Oliva

Molecular and genomic technological advancements have greatly enhanced our understanding of biological processes by allowing us to quantify key biological variables such as gene expression, protein levels, and microbiome compositions. These…

Methodology · Statistics 2024-06-12 Mingyu Du , Kevin Johnston , Veronica Berrocal , Wei Li , Xiangmin Xu , Zhaoxia Yu

Protein variability in single cells has been studied extensively in populations, but little is known about temporal protein fluctuations in a single cell over extended times. We present here traces of protein copy number measured in…

Biological Physics · Physics 2015-06-16 Naama Brenner , Erez Braun , Anna Yoney , Lee Susman , James Rotella , Hanna Salman

In this paper we propose a nonparametric graphical test based on optimal matching, for assessing the equality of multiple unknown multivariate probability distributions. Our procedure pools the data from the different classes to create a…

RNA-seq has become a de facto standard for measuring gene expression. Traditionally, RNA-seq experiments are mathematically averaged -- they sequence the mRNA of individuals from different treatment groups, hoping to correlate phenotype…

Quantitative Methods · Quantitative Biology 2013-09-05 Surojit Biswas , Yash N. Agrawal , Tatiana S. Mucyn , Jeffery L. Dangl , Corbin D. Jones

Flow cytometry (FCM) is the standard multi-parameter assay for measuring single cell phenotype and functionality. It is commonly used for quantifying the relative frequencies of cell subsets in blood and disaggregated tissues. A typical…

Applications · Statistics 2020-09-01 Shai Gorsky , Cliburn Chan , Li Ma

An expression quantitative trait locus (eQTL) is a chromosomal region where genetic variants are associated with the expression levels of certain genes that can be both nearby or distant. The identifications of eQTLs for different tissues,…

Genomics · Quantitative Biology 2023-02-24 Jingfei Zhang , Hongyu Zhao

SELEX (Systematic Evolution of Ligands by Exponential Enrichment) is an experimental procedure that allows extracting, from an initially random pool of DNA, those oligomers with high affinity for a given DNA-binding protein. We address what…

Genomics · Quantitative Biology 2009-11-11 Marko Djordjevic , Anirvan M. Sengupta

The application of single-cell molecular profiling coupled with spatial technologies has enabled charting cellular heterogeneity in reference tissues and in disease. This new wave of molecular data has highlighted the expected diversity of…

Tissues and Organs · Quantitative Biology 2024-03-12 Ricardo Omar Ramirez Flores , Philipp Sven Lars Schäfer , Leonie Küchenhoff , Julio Saez-Rodriguez

Objective: Quantitative real-time PCR is widely used for gene expression analysis, yet inconsistencies in data storage and reporting limit reproducibility. While MIQE guidelines define the minimal information required for publication, they…

Quantitative Methods · Quantitative Biology 2025-10-16 Mirco Castoldi

Gene-based testing is a commonly employed strategy in many genetic association studies. Gene-trait associations can be complex due to underlying population heterogeneity, gene-environment interactions, and various other reasons. Existing…

Methodology · Statistics 2020-12-15 Tianying Wang , Iuliana Ionita-Laza , Ying Wei

Single-molecule stretching experiments on DNA, RNA, and other biological macromolecules opened up the possibility of an impressive progress in many fields of Life and Medical sciences. The reliability of such experiments may be crucially…

Soft Condensed Matter · Physics 2019-03-22 G. Florio , G Puglisi

Pathogenic infections pose a significant threat to global health, affecting millions of people every year and presenting substantial challenges to healthcare systems worldwide. Efficient and timely testing plays a critical role in disease…

Quantitative Methods · Quantitative Biology 2023-08-03 Ananthan Nambiar , Chao Pan , Vishal Rana , Mahdi Cheraghchi , João Ribeiro , Sergei Maslov , Olgica Milenkovic

Spatial Transcriptomics enables mapping of gene expression within its native tissue context, but current platforms measure only a limited set of genes due to experimental constraints and excessive costs. To overcome this, computational…

Genomics · Quantitative Biology 2025-11-20 Amit Kumar , Maninder Kaur , Raghvendra Mall , Sukrit Gupta

It is tempting to believe that we now own the genome. The ability to read and re-write it at will has ushered in a stunning period in the history of science. Nonetheless, there is an Achilles heel exposed by all of the genomic data that has…