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Clustered regularly interspaced short palindromic repeats (CRISPR) in bacterial and archaeal DNA have recently been shown to be a new type of anti-viral immune system in these organisms. We here study the diversity of spacers in CRISPR…

Populations and Evolution · Quantitative Biology 2010-08-17 Jiankui He , Michael W. Deem

DNA microarrays are a relatively new technology that can simultaneously measure the expression level of thousands of genes. They have become an important tool for a wide variety of biological experiments. One of the most common goals of DNA…

Methodology · Statistics 2013-07-02 Eric Bair

Membraneless droplets formed through liquid-liquid phase separation (LLPS) play a crucial role in mRNA storage, enabling organisms to swiftly respond to environmental changes. However, the mechanisms underlying mRNA integration and…

Important aspects of the process of information storage and retrieval in DNA and RNA, and its evolution, are the role of the anticodons and associated $t$RNA's, and correlations between anticodons and amino acids; the degeneracy of the…

Biological Physics · Physics 2007-05-23 P. D. Jarvis , J. D. Bashford

Biological evolution of a population is governed by the fitness landscape, which is a map from genotype to fitness. However, a fitness landscape depends on the organisms environment, and evolution in changing environments is still poorly…

Populations and Evolution · Quantitative Biology 2022-09-26 Suman G Das , Joachim Krug , Muhittin Mungan

The construction of synthetic biochemical circuits is an essential step for developing quantitative understanding of information processing in natural organisms. Here, we report construction and analysis of an in vitro circuit with positive…

Molecular Networks · Quantitative Biology 2012-06-28 Pakpoom Subsoontorn , Jongmin Kim , Erik Winfree

Both short interfering RNAs (siRNAs) and microRNAs (miRNAs) mediate the repression of specific sequences of mRNA through the RNA interference pathway. In the last years several experiments have supported the hypothesis that siRNAs and…

Biomolecules · Quantitative Biology 2007-05-23 Ludovica Montanucci , Piero Fariselli , Pier Luigi Martelli , Ivan Rossi , Rita Casadio

High throughput sequencing (HTS)-based technology enables identifying and quantifying non-culturable microbial organisms in all environments. Microbial sequences have enhanced our understanding of the human microbiome, the soil and plant…

Applications · Statistics 2021-03-09 Pratheepa Jeganathan , Susan P. Holmes

The latest severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variant Omicron (B.1.1.529) has ushered panic responses around the world due to its contagious and vaccine escape mutations. The essential infectivity and antibody…

Quantitative Methods · Quantitative Biology 2021-12-03 Jiahui Chen , Rui Wang , Nancy Benovich Gilby , Guo-Wei Wei

Antimicrobial peptides are a class of small, usually positively charged amphiphilic peptides that are used by the innate immune system to combat bacterial infection in multicellular eukaryotes. Antimicrobial peptides are known for their…

Biomolecules · Quantitative Biology 2015-06-12 Natalia P. Rodina , Anna N. Yudenko , Ivan N. Terterov , Igor E. Eliseev

De novo therapeutic design is challenged by a vast chemical repertoire and multiple constraints, e.g., high broad-spectrum potency and low toxicity. We propose CLaSS (Controlled Latent attribute Space Sampling) - an efficient computational…

Antiterminators are essential components of bacterial transcriptional regulation, allowing the control of gene expression in response to fluctuating environmental conditions. Among them, RNA-binding antiterminator proteins play a major role…

Molecular Networks · Quantitative Biology 2025-09-17 Diane Soussan , Ali Tahrioui , Rafael Ruiz de la Haba , Adrien Forge , Sylvie Chevalier , Olivier Lesouhaitier , Cécile Muller

Bacterial colonies are a well-known example of living active matter, exhibiting collective behaviors such as nematic alignment and collective motion that play an important role in the spread of microbial infections. While the underlying…

Motivation: Antifungal resistance has become an increasing global concern in both clinical and environmental health. Detecting known resistance mutations directly from sequencing reads, in special metagenomic samples, remains a major…

Genomics · Quantitative Biology 2026-02-20 Henrique RM Antoniolli , Lívia Kmetzsch , Charley C Staats

Antimicrobial resistance (AMR) poses a significant threat to public health by increasing mortality, extending hospital stays, and increasing healthcare costs. It affects people of all ages and affects health services, veterinary medicine,…

Dynamical Systems · Mathematics 2023-06-12 Alissen Peterson , Jhoana P. Romero-Leiton , Pablo Aguirre , Kamal R. Acharya , Bouchra Nasri

The CRISPR (clustered regularly interspaced short palindromic repeats) mechanism allows bacteria to adaptively defend against phages by acquiring short genomic sequences (spacers) that target specific sequences in the viral genome. We…

Biological Physics · Physics 2019-09-11 Serena Bradde , Marija Vucelja , Tiberiu Tesileanu , Vijay Balasubramanian

Bacteria live in environments that are continuously fluctuating and changing. Exploiting any predictability of such fluctuations can lead to an increased fitness. On longer timescales bacteria can "learn" the structure of these fluctuations…

Cell Behavior · Quantitative Biology 2021-01-05 Stefan Landmann , Caroline M. Holmes , Mikhail Tikhonov

Microbiota profiles measure the structure of microbial communities in a defined environment (known as microbiomes). In the past decade, microbiome research has focused on health applications as a result of which the gut microbiome has been…

Quantitative Methods · Quantitative Biology 2021-05-11 Benjamin Wingfield , Sonya Coleman , T. M. McGinnity , Anthony J. Bjourson

Human decision-making differs due to variation in both incentives and available information. This constitutes a substantial challenge for the evaluation of whether and how machine learning predictions can improve decision outcomes. We…

General Economics · Economics 2020-11-24 Michael Allan Ribers , Hannes Ullrich

The volume occupied by unconstrained bacterial DNA in physiological solutions exceeds 1000 times the volume of the cell. Still, it is confined to a well defined region of the cell called the nucleoid, which occupies only a fraction of the…

Biological Physics · Physics 2016-09-27 Marc Joyeux