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Formation of a bright-field microscopic image of a transparent phase object is described in terms of elementary geometrical optics. Our approach is based on the premise that image replicates the intensity distribution (real or virtual) at…

Optics · Physics 2018-02-14 A. K. Khitrin , M. A. Model

Detection of phase variations across optically transparent samples is often a difficult task. We propose and demonstrate a compact, lightweight and low cost quantitative phase contrast imager. Light diffracted from a pinhole is incident on…

Deconvolution phase microscopy enables high-contrast visualization of transparent samples through reconstructions of their transmitted phases or refractive indexes. Herein, we propose a method to extend 2D deconvolution phase microscopy to…

Optics · Physics 2022-04-20 Herve Hugonnet , Mahn Jae Lee , Yongkeun Park

In order to efficiently image a non-absorbing sample (a phase object), dedicated phase contrast optics are required. Typically, these optics are designed with the assumption that the sample is weakly scattering, implying a linear relation…

Optics · Physics 2020-10-22 Stewart Koppell , Mark Kasevich

The challenge of imaging low-density objects in an electron microscope without causing beam damage is significant in modern TEM. This is especially true for life science imaging, where the sample, rather than the instrument, still…

Optics · Physics 2025-01-29 Francisco Vega Ibáñez , Jo Verbeeck

Holography is an optical technique enabling to record phase objects. Holographic interferometry uses this faculty to make a phase object interfere with a memory of itself at a preceding time, recorded on a hologram. Interference fringes…

Fluid Dynamics · Physics 2009-11-23 Jean Colombani , Jacques Bert

The structural complexity and instability of many interference phase microscopy methods are the major obstacles toward high-precision phase measurement. In this vein, improving more efficient configurations as well as proposing new methods…

Optics · Physics 2020-01-08 Samira Ebrahimi , Masoomeh Dashtdar

A novel phase retrieval algorithm for broadband hyperspectral phase imaging from noisy intensity observations is proposed. It utilizes advantages of the Fourier Transform spectroscopy in the self-referencing optical setup and provides,…

Image and Video Processing · Electrical Eng. & Systems 2020-06-03 Igor Shevkunov , Vladimir Katkovnik , Karen Egiazarian

Phase is a fundamental resource for optical imaging but cannot be directly observed with intensity measurements. The existing methods to quantify a phase distribution rely on complex devices and structures. Here we experimentally…

Optics · Physics 2020-03-25 Tengfeng Zhu , Junyi Huang , Zhichao Ruan

We demonstrate an optical frequency analysis method using the Fourier transform of detection times of fluorescence photons emitted from a single trapped 40Ca+ ion. The response of the detected photon rate to the relative laser frequency…

Fourier ptychographic (FP) microscope is a coherent imaging method that can synthesize an image with a higher bandwidth using multiple low-bandwidth images captured at different spatial frequency regions. The method's demand for multiple…

Optics · Physics 2016-03-25 Jaebum Chung , Hangwen Lu , Xiaoze Ou , Haojiang Zhou , Changhuei Yang

Information multiplexing is important for biomedical imaging and chemical sensing. In this paper, we report a microscopy imaging technique, termed state-multiplexed Fourier ptychography (FP), for information multiplexing and coherent-state…

Optics · Physics 2014-05-12 Siyuan Dong , Radhika Shiradkar , Pariksheet Nanda , Guoan Zheng

Phase-sensitive coherent imaging exploits changes in the phases of backscattered light to observe tiny alterations of scattering structures or variations of the refractive index. But moving scatterers or a fluctuating refractive index…

In this paper, we describe the principle of a multi-aperture interferometer that uses a phase-shifting technique and is suitable for quick, snapshot imaging of astrophysical objects at extreme angular resolution through Fourier inversion. A…

Optics · Physics 2015-05-28 Francois Henault

Ptychography involves a sample being illuminated by a coherent, localised probe of illumination. When the probe interacts with the sample, the light is diffracted and a diffraction pattern is detected. Then the probe or sample is shifted…

Numerical Analysis · Mathematics 2023-05-09 Mark Philip Roach

Different imaging modalities are used to extract the diverse information carried in an optical field. Two prominent modalities include bright field and phase contrast microscopy that can visualize the amplitude and phase features of a…

One of the most prominent challenges in the field of diffractive imaging is the phase retrieval (PR) problem: In order to reconstruct an object from its diffraction pattern, the inverse Fourier transform must be computed. This is only…

Image and Video Processing · Electrical Eng. & Systems 2022-05-06 Simon Welker , Tal Peer , Henry N. Chapman , Timo Gerkmann

Phase-contrast microscopy converts the optical phase introduced by transparent, unlabeled specimens into modulation in the intensity image. Modern phase imaging techniques are capable of quantifying phase shift at each point in the field of…

An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking…

We introduce a phase imaging mechanism for scanning transmission electron microscopy that exploits the complementary intensity changes of transmitted disks at different scattering angles. For scanning transmission electron microscopy, this…

Instrumentation and Detectors · Physics 2023-07-06 Binbin Wang , David W. McComb
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