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We analyze publicly available data on Affymetrix microarrays spike-in experiments on the human HGU133 chipset in which sequences are added in solution at known concentrations. The spike-in set contains sequences of bacterial, human and…

Biomolecules · Quantitative Biology 2011-11-10 T. Heim , L. -C. Tranchevent , E. Carlon , G. T. Barkema

A blood cell lineage consists of several consecutive developmental stages from the pluri- or multipotent stem cell to a state of terminal differentiation. Despite their importance for human biology, the regulatory pathways and gene networks…

Molecular Networks · Quantitative Biology 2020-09-18 Maryam Nazarieh , Volkhard Helms , Marc P. Hoeppner , Andre Franke

High-throughput RNA sequencing (RNA-seq) is now the standard method to determine differential gene expression. Identifying differentially expressed genes crucially depends on estimates of read count variability. These estimates are…

The life of a cell is governed by highly dynamical microscopic processes. Two notable examples are the diffusion of membrane receptors and the kinetics of transcription factors governing the rates of gene expression. Different fluorescence…

Quantitative Methods · Quantitative Biology 2020-04-03 Maxime Woringer , Ignacio Izeddin , Cyril Favard , Hugues Berry

Multilevel selection occurs when short-term individual-level reproductive interests conflict with longer-term group-level fitness effects. Detecting and quantifying this phenomenon is key to understanding evolution of traits ranging from…

Populations and Evolution · Quantitative Biology 2026-01-12 Matthew Andres Moreno , Sanaz Hasanzadeh Fard , Luis Zaman , Emily Dolson

Measuring gene expression simultaneously in both hosts and symbionts offers a powerful approach to explore the biology underlying species interactions. Such dual or simultaneous RNAseq approaches have primarily been used to gain insight…

Populations and Evolution · Quantitative Biology 2022-06-28 Amanda K Hund , Peter Tiffin , Jean-Gabriel Young , Daniel I Bolnick

Cell type (e.g. pluripotent cell, fibroblast) is the end result of many complex processes that unfold due to evolutionary, developmental, and transformational stimuli. A cell's phenotype and the discrete, a priori states that define various…

Quantitative Methods · Quantitative Biology 2013-02-05 Bradly Alicea

We present a novel framework for inferring regulatory and sequence-level information from gene co-expression networks. The key idea of our methodology is the systematic integration of network inference and network topological analysis…

Molecular Networks · Quantitative Biology 2007-09-12 Anshuman Gupta , Costas D. Maranas , Reka Albert

In many transcriptomic studies, the correlation of genes might fluctuate with quantitative factors such as genetic ancestry. We propose a method that models the covariance between two variables to vary against a continuous covariate. For…

Methodology · Statistics 2021-05-03 Tae Hyun Kim , Dan Nicolae

We perform one and two-parameter numerical bifurcation analysis of a mechanotransduction model approximating the dynamics of mesenchymal stem cell differentiation into neurons, adipocytes, myocytes and osteoblasts. For our analysis, we use…

Cell Behavior · Quantitative Biology 2023-03-16 Katiana Kontolati , Constantinos Siettos

Motivation: The discovery of relationships between gene expression measurements and phenotypic responses is hampered by both computational and statistical impediments. Conventional statistical methods are less than ideal because they either…

Methodology · Statistics 2019-07-16 Lei Ding , Daniel J. McDonald

The analysis of the leukemia data from Whitehead/MIT group is a discriminant analysis (also called a supervised learning). Among thousands of genes whose expression levels are measured, not all are needed for discriminant analysis: a gene…

Biological Physics · Physics 2007-05-23 Wentian Li , Yaning Yang

Meta-analysis methods have been widely used to combine results from multiple clinical or genomic studies to increase statistical power and ensure robust and accurate conclusion. Adaptively weighted Fisher's method (AW-Fisher) is an…

Methodology · Statistics 2017-08-18 Zhiguang Huo , Shaowu Tang , Yongseok Park , George Tseng

The expression levels of many thousands of genes can be measured simultaneously by DNA microarrays (chips). This novel experimental tool has revolutionized research in molecular biology and generated considerable excitement. A typical…

Biological Physics · Physics 2007-05-23 Eytan Domany

Four reasons why you might wish to read this paper: 1. We have devised a new statistical T test to determine differentially expressed genes (DEG) in the context of microarray experiments. This statistical test adds a new member to the…

Quantitative Methods · Quantitative Biology 2007-05-23 Shu-Dong Zhang , Timothy W. Gant

Continuing improvements in computing hardware are poised to transform capabilities for in silico modeling of cross-scale phenomena underlying major open questions in evolutionary biology and artificial life, such as transitions in…

Neural and Evolutionary Computing · Computer Science 2024-06-04 Matthew Andres Moreno , Connor Yang , Emily Dolson , Luis Zaman

Here, we provide revised gene models for D. ananassae, D. yakuba, and D. simulans, which include UTRs and empirically verified intron-exon boundaries, as well as ortholog groups identified using a fuzzy reciprocal-best-hit blast comparison.…

Genomics · Quantitative Biology 2014-10-03 Rebekah L. Rogers , Ling Shao , Jaleal S. Sanjak , Peter Andolfatto , Kevin R. Thornton

Quantitative criteria are proposed to identify genes (and sets of genes) whose expression marks a specific brain region (or a set of brain regions). Gene-expression energies, obtained for thousands of mouse genes by numerization of in-situ…

Quantitative Methods · Quantitative Biology 2011-05-09 Pascal Grange , Partha P. Mitra

Gene expression levels in a population vary extensively across tissues. Such heterogeneity is caused by genetic variability and environmental factors, and is expected to be linked to disease development. The abundance of experimental data…

Machine Learning · Statistics 2015-06-26 Zi Wang , Wei Yuan , Giovanni Montana