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We study simple mathematical models of gene expression to explore the possible origins of haploinsufficiency (HI). In a diploid organism, each gene exists in two copies and when one of these is mutated, the amount of proteins synthesized is…

Other Quantitative Biology · Quantitative Biology 2007-05-23 Indrani Bose , Rajesh Karmakar

Protein variability in single cells has been studied extensively in populations, but little is known about temporal protein fluctuations in a single cell over extended times. We present here traces of protein copy number measured in…

Biological Physics · Physics 2015-06-16 Naama Brenner , Erez Braun , Anna Yoney , Lee Susman , James Rotella , Hanna Salman

Stochastic dynamics govern many important processes in cellular biology, and an underlying theoretical approach describing these dynamics is desirable to address a wealth of questions in biology and medicine. Mathematical tools exist for…

Quantitative Methods · Quantitative Biology 2016-02-17 Iain G. Johnston , Nick S. Jones

Recent experiments using fluorescence spectroscopy have been able to probe the dynamics of conformational fluctuations in proteins. The fluctuations are Gaussian but do not decay exponentially, and are therefore, non-Markovian. We present a…

Statistical Mechanics · Physics 2008-10-14 Arti Dua , R. Adhikari

The copy number of any protein fluctuates among cells in a population; characterizing and understanding these fluctuations is a fundamental problem in biophysics. We show here that protein distributions measured under a broad range of…

Protein distributions measured under a broad set of conditions in bacteria and yeast were shown to exhibit a common skewed shape, with variances depending quadratically on means. For bacteria these properties were reproduced by temporal…

Biological Physics · Physics 2015-10-28 Naama Brenner , C. M. Newman , Dino Osmanovic , Yitzhak Rabin , Hanna Salman , D. L. Stein

A common model of stochastic auto-regulatory gene expression describes promoter switching via cooperative protein binding, effective protein production in the active state and dilution of proteins. Here we consider an extension of this…

Subcellular Processes · Quantitative Biology 2020-04-07 James Holehouse , Abhishek Gupta , Ramon Grima

Doubly stochastic Poisson processes, also known as the Cox processes, frequently occur in various scientific fields. In this article, motivated primarily by analyzing Cox process data in biophysics, we propose a nonparametric kernel-based…

Applications · Statistics 2011-01-07 Tingting Zhang , S. C. Kou

In genetic circuits, when the mRNA lifetime is short compared to the cell cycle, proteins are produced in geometrically-distributed bursts, which greatly affects the cellular switching dynamics between different metastable phenotypic…

Statistical Mechanics · Physics 2016-05-25 Shay Be'er , Metar Heller-Algazi , Michael Assaf

Biochemical reaction networks are subjected to large fluctuations attributable to small molecule numbers, yet underlie reliable biological functions. Most theoretical approaches describe them as purely deterministic or stochastic dynamical…

Molecular Networks · Quantitative Biology 2013-06-11 Jingkui Wang , Marc Lefranc , Quentin Thommen

The stochastic mutual repressor model is analysed using perturbation methods. This simple model of a gene circuit consists of two genes and three promotor states. Either of the two protein products can dimerize, forming a repressor molecule…

Biological Physics · Physics 2014-06-12 Jay Newby

Replication of genetic material is an important process for all living organisms. Origins of replication initiate the copying of DNA at many points on a chromosome, and it is the distribution of these points that is relevant here, as it…

Quantitative Methods · Quantitative Biology 2025-10-16 Huw Day , N C Snaith

Recent experiments have shown that stochastic effects exerted at the level of translation contribute a substantial portion of the variation in abundance of proteins expressed at moderate to high levels. This study analyzes translational…

Subcellular Processes · Quantitative Biology 2014-09-16 Sandip Datta , Brian Seed

In this paper we introduce a general stochastic representation for an important class of processes with resetting. It allows to describe any stochastic process intermittently terminated and restarted from a predefined random or non-random…

Probability · Mathematics 2023-10-11 Marcin Magdziarz , Kacper Taźbierski

We consider stochastic thermodynamics as a theory of statistical inference for experimentally observed fluctuating time-series. To that end, we introduce a general framework for quantifying the knowledge about the dynamical state of the…

Statistical Mechanics · Physics 2015-05-19 Bernhard Altaner , Jürgen Vollmer

Gene expression (GE) is an inherently random or stochastic or noisy process. The randomness in different steps of GE, e.g., transcription, translation, degradation, etc., leading to cell-to-cell variations in mRNA and protein levels. This…

Molecular Networks · Quantitative Biology 2021-01-12 Rajesh Karmakar , Amit Kumar Das

Mathematical models of biological neural networks are associated to a rich and complex class of stochastic processes. In this paper, we consider a simple {\em plastic} neural network whose {\em connectivity/synaptic strength} $(W(t))$…

Probability · Mathematics 2021-06-30 Philippe Robert , Gaetan Vignoud

We describe a novel coarse-grained simulation method for modelling the dynamics of globular macromolecules, such as proteins. The macromolecule is treated as a continuum that is subject to thermal fluctuations. The model includes a…

Computational Physics · Physics 2015-06-11 Robin Oliver , Daniel J. Read , Oliver G. Harlen , Sarah A. Harris

Single-cell gene expression measurements encode variability spanning molecular noise, cell-to-cell heterogeneity, and technical artifacts. Mechanistic stochastic models provide powerful approaches to disentangle these sources, yet inferring…

Quantitative Methods · Quantitative Biology 2025-09-19 Christopher E. Miles

Stochastic fluctuations in the copy number of gene products have perceivable effects on the functioning of gene regulatory networks (GRN). The Master equation (ME) provides a theoretical basis for studying such effects. However, solving the…

Molecular Networks · Quantitative Biology 2018-10-31 Jaroslav Albert
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