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We probe the dynamic strength of multiple biotin-streptavidin adhesion bonds under linear loading using the biomembrane force probe setup for dynamic force spectroscopy. Measured rupture force histograms are compared to results from a…

Biomolecules · Quantitative Biology 2010-02-24 T. Erdmann , S. Pierrat , P. Nassoy , U. S. Schwarz

The forced rupture of single chemical bonds under external load is addressed. A general framework is put forward to optimally utilize the experimentally observed rupture force data for estimating the parameters of a theoretical model. As an…

Biological Physics · Physics 2009-11-13 S. Getfert , P. Reimann

Dynamic force spectroscopy of single molecules is described by a model which predicts a distribution of rupture forces, the corresponding mean rupture force and variance, all amenable to experimental tests. The distribution has a pronounced…

Soft Condensed Matter · Physics 2007-05-23 O. K. Dudko , A. E. Filippov , J. Klafter , M. Urbakh

Biological adhesion often involves several pairs of specific receptor-ligand molecules. Using rate equations, we study theoretically the rupture of such multiple parallel bonds under dynamic loading assisted by thermal activation. For a…

Biological Physics · Physics 2009-11-06 Udo Seifert

Curvatures in the most probable rupture force ($f^*$) versus log-loading rate ($\log{r_f}$) observed in dynamic force spectroscopy (DFS) on biomolecular complexes are interpreted using a one-dimensional free energy profile with multiple…

Biological Physics · Physics 2015-06-04 Changbong Hyeon , D. Thirumalai

Heterogeneity in biological molecules, resulting in molecule-to-molecule variations in their dynamics and function, is an emerging theme. To elucidate the consequences of heterogeneous behavior at the single molecule level, we propose an…

Biological Physics · Physics 2017-01-24 Changbong Hyeon , Michael Hinczewski , D. Thirumalai

The problem of diffusive bond-dissociation in a double well potential under application of an external force is scrutinized. We compute the probability distribution of rupture forces and present a detailed discussion of the influence of…

Soft Condensed Matter · Physics 2009-11-13 Gregor Diezemann , Andreas Janshoff

Bond rupture under the action of external forces is induced by temperature fluctuations. We show that measured forces from single molecule force spectroscopy experiments can be predicted from two quantities describing the bond that are the…

Materials Science · Physics 2026-02-27 Pooja Bhat , Wafa Maftuhin , Michael Walter

Analysis of bond rupture data from single-molecule force spectroscopy experiments commonly relies on the strong assumption that the bond dissociation process is irreversible. However, with increased spatiotemporal resolution of instruments…

Biological Physics · Physics 2022-05-13 Jakob Tómas Bullerjahn , Gerhard Hummer

Using Langevin Dynamic simulations, we study effects of the shear force on the rupture of a double stranded DNA molecule. The model studied here contains two single diblock copolymers interacting with each other. The elastic constants of…

Soft Condensed Matter · Physics 2015-07-02 R. K. Mishra , T. Modi , D. Giri , S. Kumar

Single-molecule force spectroscopy experiments, as well as a number of other physical systems, are governed by thermally activated transitions out of a metastable state under the action of a steadily increasing external force. The main…

Soft Condensed Matter · Physics 2009-11-13 Sebastian Getfert , Mykhaylo Evstigneev , Peter Reimann

We consider reversible breaking of adhesion bonds or folding of proteins under the influence of a constant external force. We discuss the stochastic properties of the unbinding/rebinding events and analyze their mean number and their…

Soft Condensed Matter · Physics 2009-11-13 Gregor Diezemann , Andreas Janshoff

Using the atomic force microscope based break junction approach, applicable to metal point contacts and single molecule junctions, measurements can be repeated thousands of times resulting in rich data sets characterizing the properties of…

Mesoscale and Nanoscale Physics · Physics 2017-03-08 Mark S. Hybertsen

Experimental studies of systems containing active proteins that undergo conformational changes driven by catalytic chemical reactions have shown that the diffusion coefficients of passive tracer particles and active molecules are larger…

Soft Condensed Matter · Physics 2017-02-27 Matthew Dennison , Raymond Kapral , Holger Stark

Understanding the many-body dynamics of isolated quantum systems is one of the central challenges in modern physics. To this end, the direct experimental realization of strongly correlated quantum systems allows one to gain insights into…

We study the cooperative rupture of multiple adhesion bonds under shared linear loading. Simulations of the appropriate Master equation are compared with numerical integration of a rate equation for the mean number of bonds and its scaling…

Soft Condensed Matter · Physics 2007-05-23 T. Erdmann , U. S. Schwarz

Single receptor-ligand bonds have finite lifetimes, so that biological systems can dynamically react to changes in their environment. In cell adhesion, adhesion bonds usually act cooperatively in adhesion clusters. Outside the cellular…

Soft Condensed Matter · Physics 2007-05-23 T. Erdmann , U. S. Schwarz

Experiments measuring contact formation between a probe and quencher in disordered chains provide information on the fundamental dynamical timescales relevant to protein folding, but their interpretation usually relies on simplified…

Soft Condensed Matter · Physics 2016-02-17 Gul Zerze , Jeetain Mittal , Robert B. Best

Single-molecule pulling techniques have emerged as versatile tools for probing the noncovalent forces holding together the secondary and tertiary structure of macromolecules. They also constitute a way to study at the single-molecule level…

Biological Physics · Physics 2013-02-19 Ignacio Franco , Mark A. Ratner , George C. Schatz

Current-induced bond rupture is a fundamental process in nanoelectronic architectures such as molecular junctions and in scanning tunneling microscopy measurements of molecules at surfaces. The understanding of the underlying mechanisms is…

Mesoscale and Nanoscale Physics · Physics 2023-07-26 Yaling Ke , Jan Dvořák , Martin Čížek , Raffaele Borrelli , Michael Thoss
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