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We use digital holographic microscopy and Mie scattering theory to simultaneously characterize and track individual colloidal particles. Each holographic snapshot provides enough information to measure a colloidal sphere's radius and…

We introduce a novel framework that incorporates multiple scattering for large-scale 3D particle-localization using single-shot in-line holography. Traditional holographic techniques rely on single-scattering models which become inaccurate…

Signal Processing · Electrical Eng. & Systems 2019-06-19 Waleed Tahir , Ulugbek S. Kamilov , Lei Tian

In-line holographic microscopy provides an unparalleled wealth of information about the properties of colloidal dispersions. Analyzing one colloidal particle's hologram with the Lorenz-Mie theory of light scattering yields the particle's…

Soft Condensed Matter · Physics 2020-02-25 Lauren E. Altman , David G. Grier

In-line digital holography is a valuable tool for sizing, locating and tracking micro- or nano-objects in a volume. When a parametric imaging model is available, Inverse Problems approaches provide a straightforward estimate of the object…

Instrumentation and Detectors · Physics 2023-07-19 Nicolas Verrier , Corinne Fournier , Thierry Fournel

An in-line hologram of a colloidal sphere can be analyzed with the Lorenz-Mie theory of light scattering to measure the sphere's three-dimensional position with nanometer-scale precision while also measuring its diameter and refractive…

Soft Condensed Matter · Physics 2020-12-29 Lauren E. Altman , Rushna Quddus , Fook Chiong Cheong , David G. Grier

Three-dimensional (3D) high-resolution imaging is essential in microscopy, yet light scattering poses significant challenges in achieving it. Here, we present an approach to holographic imaging of spatially incoherent objects through…

Optics · Physics 2025-02-04 YoonSeok Baek , Hilton B. de Aguiar , Sylvain Gigan

Holographic molecular binding assays use holographic video microscopy to directly detect molecules binding to the surfaces of micrometer-scale colloidal beads by monitoring associated changes in the beads' light-scattering properties.…

Soft Condensed Matter · Physics 2020-06-24 Lauren E. Altman , David G. Grier

We propose an autofocusing algorithm to obtain, relatively accurately, the 3D position of each particle, particularly its axial location, and particle number of a dense transparent particle solution via its hologram. First, morphological…

Signal Processing · Electrical Eng. & Systems 2025-03-25 Wei-Na Li , Yi Zhou , Jiatai Chen , Hongjie Ou , XiangSheng Xie

We experimentally test a recently proposed holographic method for imaging coherent light scatterers which are distributed over a 2-dimensional grid. In our setup the scatterers consist of a back-illuminated, opaque mask with submicron-sized…

This paper presents an application of digital Fourier holography for selective imaging of scatterers with different sizes in turbid media such as biological tissues. A combination of Fourier holography and high-resolution digital recording,…

Optics · Physics 2007-05-23 Sergey A. Alexandrov , P. Meredith , T. J. McIntyre , A. V. Zvyagin

Non-invasive and single-shot holographic imaging through complex media is technically challenging due to random light scattering which significantly scrambles optical information. Recently, several methods have been presented to address…

We propose a three-dimensional nonlinear diffusion method to implement the similar autofocusing function of multiple micro-objects and simultaneously remove the defocused images, which can distinguish the locations of certain sized…

Computer Vision and Pattern Recognition · Computer Science 2019-08-15 Wei-Na Li , Zhengyun Zhang , Jianshe Ma , Xiaohao Wang , Ping Su

Holographic particle characterization uses quantitative analysis of holographic microscopy data to precisely and rapidly measure the diameter and refractive index of individual colloidal spheres in their native media. When this technique is…

Soft Condensed Matter · Physics 2022-10-19 Rafe Abdulali , Lauren E. Altman , David G. Grier

Confocal microscopy of colloids combined with digital image processing has become a powerful tool in soft matter physics and materials science. Together, these techniques enable locating and tracking of more than half a million individual…

Soft Condensed Matter · Physics 2016-05-10 Katharine E. Jensen , Nobutomo Nakamura

Holograms of colloidal particles can be analyzed with the Lorenz-Mie theory of light scattering to measure individual particles' three-dimensional positions with nanometer precision while simultaneously estimating their sizes and refractive…

Soft Condensed Matter · Physics 2018-07-04 Mark D. Hannel , Aidan Abdulali , Michael O'Brien , David G. Grier

The on-demand tailoring of light's spatial shape is of great relevance in a wide variety of research areas. Computer-controlled devices, such as Spatial Light Modulators (SLMs) or Digital Micromirror Devices (DMDs), offer a very accurate,…

We propose a novel approach to site-resolved detection of a 2D gas of ultracold atoms in an optical lattice. A near resonant laser beam is coherently scattered by the atomic array and its interference pattern is holographically recorded by…

Colloidal lithography [1] is how patterns are reproduced in a variety of natural systems and is used more and more as an efficient fabrication tool in bio-, opto-, and nano-technology. Nanoparticles in the colloid are made to form a mask on…

Mesoscale and Nanoscale Physics · Physics 2013-11-27 A. Iovan , M. Fischer , R. Lo Conte , V. Korenivski

We provide detailed experimental guidelines for implementing digital holography in the context of high-sensitivity interferometric scattering (iSCAT) based nanosizing applications. Our approach relies on interferometry via the highly…

Optics · Physics 2025-08-06 Jaime Ortega Arroyo , Matz Liebel

We develop a novel algorithm for large-scale holographic reconstruction of 3D particle fields. Our method is based on a multiple-scattering beam propagation method (BPM) combined with sparse regularization that enables recovering dense 3D…

Optics · Physics 2021-05-24 Hao Wang , Waleed Tahir , Jiabei Zhu , Lei Tian
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