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Beam damage is a major limitation in electron microscopy that becomes increasingly severe at higher resolution. One possible route to circumvent radiation damage, which forms the basis for single-particle electron microscopy and related…

We present a deep learning, computer vision algorithm constructed for the purposes of identifying and classifying charged particles in camera image sensors. We apply our algorithm to data collected by the Distributed Electronic Cosmic-ray…

A practical method utilising three-dimensional image pattern matching is proposed which, in principle, is capable of unambiguous determination of the types and positions of atoms in small molecules from defocus series collected at only a…

Image and Video Processing · Electrical Eng. & Systems 2019-12-02 T. E. Gureyev , H. M. Quiney , A. Kozlov , L. J. Allen

A common task in cryo-electron microscopy (cryo-EM) data processing is to compare three-dimensional density maps of macromolecules. In this paper, we propose an algorithm for aligning three-dimensional density maps that exploits common…

Image and Video Processing · Electrical Eng. & Systems 2023-02-27 Yael Harpaz , Yoel Shkolnisky

Cellular Electron Cryo-Tomography (CECT) is a powerful 3D imaging tool for studying the native structure and organization of macromolecules inside single cells. For systematic recognition and recovery of macromolecular structures captured…

Computer Vision and Pattern Recognition · Computer Science 2018-07-04 Chang Liu , Xiangrui Zeng , Kaiwen Wang , Qiang Guo , Min Xu

Automatic classification of aquatic microorganisms is based on the morphological features extracted from individual images. The current works on their classification do not consider the inter-class similarity and intra-class variance that…

Computer Vision and Pattern Recognition · Computer Science 2021-09-27 Aishwarya Venkataramanan , Martin Laviale , Cécile Figus , Philippe Usseglio-Polatera , Cédric Pradalier

The recent improvements in cryo-electron microscopy (cryo-EM) in the past few years are now allowing to observe molecular complexes at atomic resolution. As a consequence, numerous structures derived from cryo-EM are now available in the…

Biomolecules · Quantitative Biology 2020-05-04 Mikael Trellet , Gydo van Zundert , Alexandre M. J. J. Bonvin

Cryo-electron tomography (Cryo-ET) is a powerful tool in structural biology for 3D visualization of cells and biological systems at resolutions sufficient to identify individual proteins in situ. The measurements are collected by tilting…

Signal Processing · Electrical Eng. & Systems 2025-09-03 Vinith Kishore , Valentin Debarnot , AmirEhsan Khorashadizadeh , Ivan Dokmanić

Microorganisms play a vital role in human life. Therefore, microorganism detection is of great significance to human beings. However, the traditional manual microscopic detection methods have the disadvantages of long detection cycle, low…

Computer Vision and Pattern Recognition · Computer Science 2022-04-12 Pingli Ma , Chen Li , Md Mamunur Rahaman , Yudong Yao , Jiawei Zhang , Shuojia Zou , Xin Zhao , Marcin Grzegorzek

Cellular electron cryo-tomography enables the 3D visualization of cellular organization in the near-native state and at submolecular resolution. However, the contents of cellular tomograms are often complex, making it difficult to…

Quantitative Methods · Quantitative Biology 2017-12-29 Xiangrui Zeng , Miguel Ricardo Leung , Tzviya Zeev-Ben-Mordehai , Min Xu

In the field of integrated circuit manufacturing, the detection and classification of nanoscale wafer defects are critical for subsequent root cause analysis and yield enhancement. The complex background patterns observed in scanning…

Computer Vision and Pattern Recognition · Computer Science 2025-02-24 Qian Jin , Yuqi Jiang , Xudong Lu , Yumeng Liu , Yining Chen , Dawei Gao , Qi Sun , Cheng Zhuo

The recent growth in data volumes produced by modern electron microscopes requires rapid, scalable, and flexible approaches to image segmentation and analysis. Few-shot machine learning, which can richly classify images from a handful of…

Microscopy images contain rich information about how cells respond to perturbations, making them essential to applications like drug screening. To quantify images, researchers often use representation extraction methods, and recent years…

Computer Vision and Pattern Recognition · Computer Science 2026-05-18 Emre Hayir , Lorin Crawford , Alex X. Lu

The construction industry represents a major sector in terms of resource consumption. Recycled construction material has high reuse potential, but quality monitoring of the aggregates is typically still performed with manual methods.…

Computer Vision and Pattern Recognition · Computer Science 2025-08-06 Yu Zhou , Pelle Thielmann , Ayush Chamoli , Bruno Mirbach , Didier Stricker , Jason Rambach

Cells segmentation shows rapid growth in biology. Indeed, using the classical segmentation methods only is not enough to segment this type of images. In this manuscript, we will present a new method of ribosomes segmentation. A…

Image and Video Processing · Electrical Eng. & Systems 2021-01-14 Mariam El Oussini

Cryo-electron tomography (cryo-ET) enables structural characterization of biomolecules under near-native conditions. Existing approaches for interpreting the resulting three-dimensional volumes are computationally expensive and have…

Quantitative Methods · Quantitative Biology 2025-10-28 Chengxuan Li , August George , Reece Neff , Doo Nam Kim , Trevor Moser , Kate Baldwin , Malio Nelson , Arsam Firoozfar , James E Evans , Margaret S Cheung

Cryo-electron tomography enables 3D visualization of cells in a near native state at molecular resolution. The produced cellular tomograms contain detailed information about all macromolecular complexes, their structures, their abundances…

Quantitative Methods · Quantitative Biology 2019-04-30 Min Xu , Elitza I Tocheva , Yi-Wei Chang , Grant J Jensen , Frank Alber

Automated sample preparation and electron microscopy enables acquisition of very large image data sets. These technical advances are of special importance to the field of neuroanatomy, as 3D reconstructions of neuronal processes at the nm…

Deep learning has become an extremely effective tool for image classification and image restoration problems. Here, we apply deep learning to microscopy, and demonstrate how neural networks can exploit the chromatic dependence of the…

Optics · Physics 2018-07-05 Eran Hershko* , Lucien E. Weiss* , Tomer Michaeli , Yoav Shechtman

Cryo-EM is a powerful tool for understanding macromolecular structures, yet current methods for structure reconstruction are slow and computationally demanding. To accelerate research on pose estimation, we present CESPED, a new dataset…

Quantitative Methods · Quantitative Biology 2024-05-03 Ruben Sanchez-Garcia , Michael Saur , Javier Vargas , Carl Poelking , Charlotte M Deane