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Recent genome-wide measurements of binding preferences of ~200 transcription regulators in the vicinity of transcription start sites in yeast, have provided a unique insight into the cis- regulatory code of a eukaryotic genome (Venters et…

Biomolecules · Quantitative Biology 2012-04-19 Ariel Afek , David B. Lukatsky

Quantitative understanding of the principles regulating nucleosome occupancy on a genome-wide level is a central issue in eukaryotic genomics. Here, we address this question using budding yeast, Saccharomyces cerevisiae, as a model…

Biomolecules · Quantitative Biology 2011-11-22 Ariel Afek , Itamar Sela , Noa Musa-Lempel , David B. Lukatsky

Genome-wide experiments to map the DNA-binding locations of transcription-associated factors (TFs) have shown that the number of genes bound by a TF far exceeds the number of possible direct target genes. Distinguishing functional from…

Genomics · Quantitative Biology 2022-11-29 Christopher J. Banks , Anagha Joshi , Tom Michoel

Transcription factors (TFs) are macromolecules that bind to \textit{cis}-regulatory specific sub-regions of DNA promoters and initiate transcription. Finding the exact location of these binding sites (aka motifs) is important in a variety…

Computer Vision and Pattern Recognition · Computer Science 2016-11-18 Hamid Reza Hassanzadeh , May D. Wang

Transcription factors (TFs) interact with a multitude of binding sites on DNA and partner proteins inside cells. We investigate how nonspecific binding/unbinding to such decoy binding sites affects the magnitude and time-scale of random…

Subcellular Processes · Quantitative Biology 2015-04-15 Mohammad Soltani , Pavol Bokes , Zachary Fox , Abhyudai Singh

Transcription factors (TFs) regulate gene expression by binding to specific genomic loci determined by DNA sequence. Their sequence specificity is commonly summarized by a consensus binding motif. However, eukaryotic genomes contain…

Transcription factors (TFs) regulate gene expression through complex and co-operative mechanisms. While many TFs act together, the logic underlying TFs binding and their interactions is not fully understood yet. Most current approaches for…

Machine Learning · Computer Science 2026-03-13 Pietro Demurtas , Ferdinando Zanchetta , Giovanni Perini , Rita Fioresi

Transcription regulation is largely governed by the profile and the dynamics of transcription factors' binding to DNA. Stochastic effects are intrinsic to this dynamics and the binding to functional sites must be controled with a certain…

Genomics · Quantitative Biology 2007-05-23 Erik Aurell , Aymeric Fouquier d'Herouel , Claes Malmnas , Massimo Vergassola

The speed of site-specific binding of transcription factor (TFs) proteins with genomic DNA seems to be strongly retarded by the randomly occurring sequence traps. Traps are those DNA sequences sharing significant similarity with the…

Subcellular Processes · Quantitative Biology 2016-07-22 G. Niranjani , R. Murugan

The binding of transcription factors (TFs) is essential for gene expression. One important characteristic is the actual occupancy of a putative binding site in the genome. In this study, we propose an analytical model to predict genomic…

Quantitative Methods · Quantitative Biology 2015-01-14 Nicolae Radu Zabet , Boris Adryan

Transcription factors (TFs) exert their regulatory action by binding to DNA with specific sequence preferences. However, different TFs can partially share their binding sequences due to their common evolutionary origin. This `redundancy' of…

Genomics · Quantitative Biology 2018-12-05 Antonio Rosanova , Alberto Colliva , Matteo Osella , Michele Caselle

The binding of a transcription factor (TF) to a DNA operator site can initiate or repress the expression of a gene. Computational prediction of sites recognized by a TF has traditionally relied upon knowledge of several cognate sites,…

Biomolecules · Quantitative Biology 2008-11-10 Sahand Jamal Rahi , Peter Virnau , Leonid A. Mirny , Mehran Kardar

We predict analytically that diagonal correlations of amino acid positions within protein sequences statistically enhance protein propensity for nonspecific binding. We use the term 'promiscuity' to describe such nonspecific binding.…

Biomolecules · Quantitative Biology 2011-08-16 David B. Lukatsky , Ariel Afek , Eugene I. Shakhnovich

Transcription factors (TFs) are proteins that bind to specific sites on the DNA and regulate gene activity. Identifying where TF molecules bind and how much time they spend on their target sites is key for understanding transcriptional…

Molecular Networks · Quantitative Biology 2013-10-01 Nicolae Radu Zabet , Robert Foy , Boris Adryan

One goal of human genetics is to understand how the information for precise and dynamic gene expression programs is encoded in the genome. The interactions of transcription factors (TFs) with DNA regulatory elements clearly play an…

Genomics · Quantitative Biology 2014-04-15 Darren A. Cusanovich , Bryan Pavlovic , Jonathan K. Pritchard , Yoav Gilad

We introduce a novel method to screen the promoters of a set of genes with shared biological function, against a precompiled library of motifs, and find those motifs which are statistically over-represented in the gene set. The gene sets…

Molecular Networks · Quantitative Biology 2008-11-11 Yuval Tabach , Ran Brosh , Yossi Buganim , Anat Reiner , Or Zuk , Assif Yitzhaky , Mark Koudritsky , Varda Rotter , Eytan Domany

We develop revised theoretical ideas on the mechanism by which the transcription factor proteins locate their specific binding sites on DNA faster than the three-dimensional (3D) diffusion controlled rate limit. We demonstrate that the…

Biological Physics · Physics 2014-09-22 Rajamanickam Murugan

Evolution of gene regulation is crucial for our understanding of the phenotypic differences between species, populations and individuals. Sequence-specific binding of transcription factors to the regulatory regions on the DNA is a key…

Populations and Evolution · Quantitative Biology 2015-11-09 Murat Tuğrul , Tiago Paixão , Nicholas H. Barton , Gašper Tkačik

Transcription factor binding to the surface of DNA regulatory regions is one of the primary causes of regulating gene expression levels. A probabilistic approach to model protein-DNA interactions at the sequence level is through Position…

Biological Physics · Physics 2015-11-18 Jacob Clifford , Christoph Adami

Response time and transcription level are vital parameters of gene regulation. They depend on how fast transcription factors (TFs) find and how efficient they occupy their specific target sites. It is well known that target site search is…

Biological Physics · Physics 2018-06-18 Johannes Hettich , J. Christof M. Gebhardt
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