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We study the control parameters that govern the dynamics of in vitro DNA ejection in bacteriophage lambda. Past work has demonstrated that bacteriophage DNA is highly pressurized; this pressure has been hypothesized to help drive DNA…

Biomolecules · Quantitative Biology 2015-05-18 David Wu , David Van Valen , Qicong Hu , Rob Phillips

Bacteriophages spreading through populations of bacteria offer relatively simple, tuneable systems for testing mathematical models of range expansion. However, such models typically assume a static state into which to expand, which is not…

Soft Condensed Matter · Physics 2024-04-02 Rory Claydon , Samuel Gartenstein , Aidan T. Brown

During the lifecycle of many single-stranded RNA viruses, including many human pathogens, a protein shell called the capsid spontaneously assembles around the viral genome. Understanding the mechanisms by which capsid proteins selectively…

Biomolecules · Quantitative Biology 2015-02-19 J. D. Perlmutter , M. F. Hagan

We present a simple kinetic model for the assembly of small single-stranded RNA viruses that can be used to carry out analytical packaging contests between different types of RNA molecules. The RNA selection mechanism is purely kinetic and…

Biological Physics · Physics 2021-02-09 Inbal Mizrahi , Robijn Bruinsma , Joseph Rudnick

Gene regulatory network of lambda phage is one the best studied model systems in molecular biology. More 50 years of experimental study has provided a tremendous amount of data at all levels: physics, chemistry, DNA, protein, and function.…

Molecular Networks · Quantitative Biology 2007-05-23 X. -M. Zhu , L. Yin , L. Hood , P. Ao

The coat proteins of many viruses spontaneously form icosahedral capsids around nucleic acids or other polymers. Elucidating the role of the packaged polymer in capsid formation could promote biomedical efforts to block viral replication…

Biomolecules · Quantitative Biology 2015-05-19 Oren M. Elrad , Michael F. Hagan

Bacteriophages (or phages), which infect bacteria, have two distinct lifestyles: virulent and temperate. Predicting the lifestyle of phages helps decipher their interactions with their bacterial hosts, aiding phages' applications in fields…

Genomics · Quantitative Biology 2023-01-02 Jiayu Shang , Xubo Tang , Yanni Sun

Simple RNA viruses self-assemble spontaneously and encapsulate their genome into a shell called the capsid. This process is mainly driven by the attractive electrostatic interaction between the positive charges on capsid proteins and the…

Biological Physics · Physics 2022-02-22 Sanaz Panahandeh , Siyu Li , Bogdan Dragnea , Roya Zandi

Molecular carriers represent an increasingly common strategy in the field of nanopore sensing to use secondary molecules to selectively report on the presence of target analytes in solution, allowing for sensitive assays of otherwise…

Biomolecules · Quantitative Biology 2023-05-10 Zachary Roelen , Vincent Tabard-Cossa

Certain defense mechanisms of phages against the immune system of their bacterial host rely on cooperation of phages. Motivated by this example we analyse invasion probabilities of cooperative parasites in host populations that are…

Populations and Evolution · Quantitative Biology 2022-07-22 Vianney Brouard , Cornelia Pokalyuk

Bacterial cells exhibit a diverse array of shapes and sizes, largely governed by their cell walls in conjunction with cytoskeletal proteins and internal turgor pressure. The present study develops a theoretical framework for modeling the…

Biological Physics · Physics 2024-09-25 Joydip Chaudhuri

We explore the role of cellular life cycles for viruses and host cells in an infection process. For this purpose, we derive a generalized version of the basic model of virus dynamics (Nowak, M.A., Bangham, C.R.M., 1996. Population dynamics…

Populations and Evolution · Quantitative Biology 2008-04-28 Daniel Campos , Vicenç Méndez , Sergei Fedotov

Numerous studies have explored the link between bacterial swimming and the number of flagella, a distinguishing feature of motile multiflagellated bacteria. We revisit this open question using augmented slender-body theory simulations, in…

Biological Physics · Physics 2024-09-04 Maria Tătulea-Codrean , Eric Lauga

For many viruses assembly and budding occur simultaneously during virion formation. Understanding the mechanisms underlying this process could promote biomedical efforts to block viral propagation and enable use of capsids in nanomaterials…

Subcellular Processes · Quantitative Biology 2014-03-11 Teresa Ruiz-Herrero , Michael F. Hagan

Despite the advent of several novel, synthetic gene drive mechanisms and their potential to one-day control a number of devastating diseases, among other applications, practical use of these systems remains contentious and risky. In…

Populations and Evolution · Quantitative Biology 2018-08-02 Floyd A. Reed , Todd G. Aquino-Michaels , Maria S. Costantini , Áki J. Láruson , Jolene T. Sutton

The role of thermal pressure fluctuations in the ejection of tightly packaged DNA from protein capsid shells is discussed in a model calculation. At equilibrium before ejection we assume the DNA is folded many times into a bundle of…

Mesoscale and Nanoscale Physics · Physics 2010-09-20 Michael J. Harrison

Molecular motors play pivotal roles in organizing the interior of cells. A motor efficient in cargo transport would move along cytoskeletal filaments with a high speed and a minimal error in transport distance (or time) while consuming a…

Biological Physics · Physics 2019-06-07 Wonseok Hwang , Changbong Hyeon

Many bacteria are motile by means of one or more rotating rigid helical flagella, making them the only known organism to use rotation as a means of propulsion. The rotation is supplied by the bacterial flagellar motor, a particularly…

Navigation of microorganisms is controlled by internal processes ultimately sensitive to mechanical or chemical signaling encountered along the path. In many natural environments, such as porous soils or physiological ducts, motile species…

Cells control the size and organization of biomolecular condensates formed by liquid-liquid phase separation (LLPS), but multiple mechanisms likely contribute to this control and remain to be fully elucidated. Here we propose a…

Biological Physics · Physics 2026-04-10 Le Qiao , Peter Gispert , Lukas S. Stelzl , Friederike Schmid